Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bromelain, production method and treatment system thereof

A technology of bromelain and its production method, which is applied in the field of bromelain, its production method and its processing system, can solve the problems of high enzyme activity loss, low enzyme activity, and unimproved purity extraction rate, so as to ensure enzyme activity and reduce loss effect

Active Publication Date: 2019-11-12
CHONGQING JOYWIN NATURAL PROD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But some can only improve the yield but the enzyme activity is not high, or can only improve the enzyme activity but the purity or extraction rate has not improved, such as using ultrafiltration in CN109022402A to process, it may be possible to improve a certain yield, but using boiling water treatment, it will lead to the loss of its enzyme activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bromelain, production method and treatment system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The present embodiment provides a kind of production method of bromelain, comprises the following steps:

[0059] Take 1000kg of fresh pineapple rhizomes (the detection raw material unit enzyme activity is 7660U / g), put it into water with a water temperature of 25°C, wash it by rolling for 0.5 hours, and then put it into cold water with a water temperature of 10°C for 30 minutes, so that the temperature of the material drops to 13°C , The feeding rate is 5 tons / hour. Then the pineapple rhizome is shredded into small pieces, which are irregular sheets of 1-8cm, and then 1000kg of process water at 4°C is added, stirred for 30min (sampling and detection unit enzyme activity is 3804U / g), and then the juice is squeezed. The pressure is 0.10MPa, and the juice is about 1250kg (the unit enzyme activity of the squeezed juice is 5964U / g). Centrifuge the squeezed juice at a speed of 3100 rpm to separate 306 kg of sediment and 943 kg of crude pineapple extract.

[0060] Add 5 tim...

Embodiment 2

[0066] The present embodiment provides a kind of production method of bromelain, comprises the following steps:

[0067] Take 1000kg of fresh pineapple rhizomes (the raw material unit enzyme activity is 7500U / g), put them in water with a water temperature of 30°C, wash them by rolling for 0.2 hours, and then soak them in cold water with a water temperature of 15°C for 60 minutes to reduce the temperature of the materials to 17°C , The feeding rate is 3 tons / hour. Then the pineapple rhizomes are shredded into small pieces, which are irregular sheets of 1-8cm, and 1000kg of process water at 4-10°C is added, stirred for 60min (the unit enzyme activity of sampling detection is 3752U / g), and then, the juice is squeezed again, Juicing pressure is 0.3MPa, and the juice is about 1397kg (the unit enzyme activity of the squeezed juice is 3633U / g). Centrifuge the squeezed juice at a speed of 1500 rpm to separate and form 273 kg of precipitate and 967 kg of crude pineapple extract.

[0...

Embodiment 3

[0072] The present embodiment provides a kind of production method of bromelain, comprises the following steps:

[0073] Take 1000kg of fresh pineapple rhizomes (the raw material unit enzyme activity is 7667U / g), put them in water with a water temperature of 20°C, wash them by rolling for 0.4 hours, and then soak them in cold water with a water temperature of 8°C for 20 minutes to reduce the temperature of the materials to 12°C , The feeding rate is 10 tons / hour. Then the pineapple rhizome is shredded into small pieces, which are irregular sheets of 1-8cm, and then 1000kg of process water at 4-10°C is added, stirred for 60min (sampling detection unit enzyme activity is 3921U / g), and then the juice is squeezed. The juicing pressure is 0.3MPa, and the juice is about 1316kg (the unit enzyme activity of the squeezed juice is 6023U / g). Centrifuge the squeezed juice at a speed of 2000 rpm to separate and form 300 kg of precipitate and 962 kg of crude pineapple extract.

[0074] Ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of protease extraction, in particular to bromelain, a production method and a treatment system thereof. The production method of the bromelain comprises the following steps that concentration-filtration operation is conducted for at least one time on pineapple extract crude liquid until the mass content of solid in concentration liquid is 5%-8%; and then the concentration liquid is concentrated until the mass content of solid in the concentration liquid is 18%-20%. Through the use of concentration-filtration, the pineapple extract crude liquid can be concentrated and impurity-removed, meanwhile the mass content of the solid in the concentration liquid after concentration-filtration is controlled, the purity of the bromelain can be ensured, and the enzymeactivity of the bromelain is ensured. The solid content after concentration-filtration reaches a certain range, filtration is not conducted anymore, concentration is only conducted, the loss of the bromelain is reduced, and the enzyme activity is ensured. The solid content of the final concentration liquid is controlled, and the enzyme activity can be ensured.

Description

technical field [0001] The invention relates to the field of protease extraction, in particular to a bromelain, a production method and a treatment system thereof. Background technique [0002] Bromelain is a thiol protease extracted from pineapple juice, skin, etc. The existing general method of extracting bromelain is to squeeze fresh pineapple juice and add benzoic acid, kaolin, etc. for adsorption, then adjust the pH to 6.7-7.0 and then add sodium chloride , and then press-filtered, the filtrate was adjusted to pH 4.8-5.1, and after adding ammonium sulfate, it was allowed to stand still to form a sediment, namely bromelain, but the above extraction method was too complicated to operate, and the extraction efficiency of bromelain was low, the purity was not high, and the enzyme activity was relatively low. reduce. There are many ways to improve bromelain in the prior art. But some can only improve the yield but the enzyme activity is not high, or can only improve the en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/64C12M1/12
CPCC12M21/14C12M33/14C12N9/641C12Y304/22004C12Y304/22031
Inventor 李站龙徐小琴钱英杨立琛
Owner CHONGQING JOYWIN NATURAL PROD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com