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Tumor migration and invasion capacities and/or proliferation capacity evaluation kit

A technology of tumor migration and proliferation ability, applied in the field of tumor migration and invasion ability and/or proliferation ability evaluation kit, can solve the problems of low survival rate and poor prognosis of patients with advanced gastric cancer, achieve tumor suppression, reduce invasion and migration ability and The effect of proliferative capacity

Active Publication Date: 2019-11-12
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Even with comprehensive systemic treatment, patients with advanced gastric cancer still have a poor prognosis, with a 5-year overall survival rate of less than 20%

Method used

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  • Tumor migration and invasion capacities and/or proliferation capacity evaluation kit
  • Tumor migration and invasion capacities and/or proliferation capacity evaluation kit
  • Tumor migration and invasion capacities and/or proliferation capacity evaluation kit

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Experimental program
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Embodiment Construction

[0053] The specific implementation manners of the present invention will be described below in conjunction with the accompanying drawings. The term used in this article: arsenic sulfide, both refer to the chemical formula As 4 S 4 compound of.

[0054] 1: Gene chip detection:

[0055] 1. Sample Preparation

[0056] 1) Gastric cancer cell line AGS was divided into blank control group and 1μM arsenic sulfide treatment group for 2h, 6h, 12h, and 24h respectively, among which the blank control group and 1μM arsenic sulfide treatment group for 12h were used for gene chip detection.

[0057] 2) After the cell culture induction is over, control the dryness and remove the culture medium;

[0058] 2) According to every 10cm 2 (Equivalent to one well of a six-well plate or a 35mm diameter petri dish) add TRIzol reagent at the ratio of cells plus 1mL TRIzol reagent;

[0059] 3) Use a glass dropper (or pipette tip) to pipette the cells repeatedly until no agglomerated cell masses are ...

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Abstract

The invention provides a tumor migration and invasion capacities and / or proliferation capacity evaluation kit. The tumor migration and invasion ability and / or proliferation ability evaluation kit is characterized in that the tumor migration and invasion ability and / or proliferation ability evaluation kit includes a reagent for detecting expression levels of miR-4665-3p, miR-135b-5p, miR-4739 and / or miR-6124. The invention further provides application of the miR-4665-3p to preparation of drugs to inhibit proliferation and invasion and migration capacities of gastric cancer cells. According to the tumor migration and invasion ability and / or proliferation ability evaluation kit, the invasion and migration capacities and / or proliferation capacity of the gastric cancer cells can be judged simply and effectively, grade malignancy is helped to judge, and medication is guided.

Description

technical field [0001] The invention relates to a kit for evaluating tumor migration and invasion ability and / or proliferation ability, belonging to the field of biological detection. Background technique [0002] The occurrence of gastric cancer is a multi-step biological process, which is the second leading cause of cancer death worldwide, and the incidence of invasion and metastasis is high in the process of gastric cancer. [0003] About 50% of stomach cancers occur in Asia, especially in China, Japan and Korea. In view of the lack of awareness and effective methods for early diagnosis, many patients are unfortunately found at an advanced stage, with extensive invasion and metastasis. Even with comprehensive systemic treatment, patients with advanced gastric cancer still have a poor prognosis, with a 5-year overall survival rate of less than 20%. It is known that Helicobacter pylori infection and many genetic and environmental factors play a crucial role in the occurre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K31/7088A61P35/00A61P35/04
CPCC12Q1/6886A61K31/7088A61P35/00A61P35/04C12Q2600/158C12Q2600/178
Inventor 陈思宇谭圳张秀丽康婷朱传营
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE