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Detection method of biological soil crust polysaccharide

A biological soil and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection sensitivity and accuracy, and achieve the effects of easy promotion and application, simple equipment, and comprehensiveness

Pending Publication Date: 2019-11-12
WUHAN UNIV OF TECH
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Problems solved by technology

[0005] In view of this, the present invention aims to propose a detection method of biological soil crust polysaccharides to solve the problem of low detection sensitivity and accuracy of existing biological soil crust polysaccharides

Method used

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  • Detection method of biological soil crust polysaccharide
  • Detection method of biological soil crust polysaccharide
  • Detection method of biological soil crust polysaccharide

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Embodiment 1

[0051] A method for detecting biological soil crust polysaccharides specifically includes the following steps:

[0052] 1) Sample collection and preservation: Use a sterile shovel to collect biological soil crusts in desert areas, the shape of which is like figure 1 As shown, it is stored in a sterile plastic plate and shipped back as soon as possible. During the return process, it is bonded and bound to try to fix it, and ensure that it is stable during transportation without major fluctuations to ensure that its structure is not damaged. , Store it in a cool place after transporting it back, put it in a dry sealed jar, and seal the mouth of the jar with petroleum jelly;

[0053] 2) Use water extraction and alcohol precipitation method to extract polysaccharides from biological soil crusts to be tested: Grind 5g of biological soil crusts to be tested, pass through an 80-mesh sieve, remove leaves and other impurities, shake for 2h in a 40℃ water bath, and Centrifuge at 8000r / min to...

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Abstract

The invention provides a detection method of biological soil crust polysaccharide. On the one hand, the detection method comprises the step of extracting the biological soil crust exopolysaccharide byadopting water extraction and alcohol precipitation method, wherein the extraction method is economic and convenient, the equipment is simple, and the polysaccharide yield is high; on the other hand,the hydrolyzed polysaccharide after extraction is derivatively treated by adopting saccharin acetate derivatization method and the trimethyl silane derivatization method, the monosaccharide in different types can be detected, and the detection comprehensiveness is ensured; furthermore, the monosaccharide component after derivatization treatment is qualitatively and quantitatively analyzed by adopting gas chromatography-mass spectrometry analysis method and an internal standard method, and three are collaboratively used, so that the detection method of the biological coil crust polysaccharidecan detect the polysaccharide with high sensitivity and accuracy; and meanwhile, the detection method disclosed by the invention is easy to realize, and the method is easy to popularize and apply.

Description

Technical field [0001] The invention relates to the technical field of polysaccharide determination, in particular to a method for detecting biological soil crust polysaccharides. Background technique [0002] The arid area accounts for 41% of the world’s land area, and it stores 27% of the world’s organic carbon and 95% of the inorganic carbon. According to statistics, the amount of C stored in desert areas around the world is about 10×10 15 g, which is as high as 56×10 12 g C is stored in the cyanobacteria biomass of Biological Soil Crusts (BSCs). BSCs is a biological soil composite layer formed on the surface by cyanobacteria, lichens, moss and other organisms cementing and binding soil particles. It has important ecological functions such as improving soil moisture conditions and increasing fertility. [0003] Biological soil crust can be divided into algal crust, lichen crust and moss crust. Among them, algal crust is the initial stage and prerequisite for the subsequent for...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/065G01N2030/067
Inventor 吴丽叶兴旺钱隆林思萌杨列张祖麟
Owner WUHAN UNIV OF TECH
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