Plant culture device for experiment of inoculating plant endophytes into leaves

Abstract

The invention provides a plant culture device for an experiment of inoculating plant endophytes into leaves. The plant culture device comprises a cylindrical device body, a cover is arranged on the upper portion of the device body, a bottle opening is formed in the middle of the cover, a bacteriological-filtration breathable film is arranged at the bottle opening, an opening is formed in the upperportion of the cover, and a telescoping device is arranged at the opening. The end of the telescoping device is connected with a spraying pipe, the end, in the device body, of the spraying pipe is provided with a sprayer, and the end, outside the device body, of the spraying pipe is provided with a pressure device and a hose which extends outwards. According to the culture device, due to the design of the opening, spraying position, in a culture bottle, of the sprayer at the end of the spraying pipe can be well adjusted, and precise spraying inoculation of the cultured plant leaves is achieved. The aseptic condition in the culture bottle can be kept, the influence of other microorganisms on the biological effect of the endophytes on an experimental host plant can be reduced to the greatest extent, and the real effect of the endophytes is reflected.

Description

technical field [0001] The invention relates to the technical field of experimental devices, in particular to a plant culture device for the experiment of leaf inoculation with plant endophytes. Background technique [0002] Plant endophytes are fungi or bacteria that live in the tissues and organs of healthy plants for some or all stages. Plant endophytes are fungi that can not only produce various chemical substances, but also inhibit or kill various pathogenic bacteria through competition or other effects, and do not cause obvious disease symptoms to plant tissues, including those in the life cycle. Saprophytic bacteria that grow on the surface of the camp at a certain stage, latent pathogenic bacteria and mycorrhizal fungi that are temporarily harmless to the host, etc., can be isolated from several to dozens of endophytic bacteria on one plant. Plant endophytes are a new microbial resource with great potential application value. [0003] The traditional view is that c...

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Problems solved by technology

In the process of artificially inoculating endophytic bacteria, in order to avoid the influence of other microorganisms on the experimental endophytic inoculation, it needs to be carried out in a sterile culture environment. The current plant culture device usually has a cover with a filter and a breathable membrane on the top These devices are feasible for the above two inoculation methods ① and ②, especially the ② inoculation method only needs to place the treated seeds in the cultivation device at one time, and control the temperature externally. There is no need to open the culture device during the process of waiting for the culture conditions until the end of the experiment, so as to maintain the consistency of the culture environment well. However, the inoculation method in the above-mentioned step ③ needs to be sprayed and inoculated after the leaves grow, and the existing culture device needs to be opened. The upper cover is manually sprayed, so it is difficult to maintain the consistency of the culture environment in the existing culture device, and the experimental process is easily affected by other microorganisms

At present, there are few reports on the special culture device used for this experiment, but this culture device is a device with cutting-edge experimental significance

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