Beaded electrospun nanofiber immobilized saccharomycete and preparation method thereof

A technology for immobilizing yeast and nanofibers, which is applied in the preparation of spinning solution, immobilization on/in organic carriers, fiber processing, etc., which can solve the limitation of enzyme or cell load, difficult product separation, and low product quality. problem, to achieve the effect of rapid response, easy control and wide application

Inactive Publication Date: 2016-07-27
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The surface loading method is a method of preparing the nanofiber membrane first and then fixing it. The disadvantage of this method is that the loading capacity of the enzyme or cells is severely limited, and the activity will be greatly affected during use; while the embedding method is A method of directly mixing enzymes or cells with polymer solutions for spinning, this method makes up for the defects of the surface loading method, and greatly improves the loading capacity and enzyme activity of fiber membranes on enzymes or cells
[0005] In the fermentation process of traditional free yeast, there will be problems such as difficult separation of products, easy loss, and low product quality. In order to solve this problem, the present invention uses electrospinning technology to introduce microorganisms---yeasts into the spinning solution. Immobilization of yeast to form an active nanomicrobial composite

Method used

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  • Beaded electrospun nanofiber immobilized saccharomycete and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]1) Preparation of electrospinning spinning polymer or macromolecular mixed solution: Weigh polyacrylamide by balance, under nitrogen protection, slowly add mixed solvent ethanol / water volume ratio of 5:7, magnetically stir for 20min, control temperature at 40 °C to make it mix evenly to form a homogeneous solution, and finally configure it into a polyacrylamide mixed solution with a mass concentration of 10wt%;

[0032] 2) Preparation of electrospinning spinning solution containing yeast: Weigh 0.2 g of yeast solid particles, add it to the mixed solution in step 1, and stir magnetically for 20 minutes to fully disperse the yeast cells in the mixed solution, and finally obtain Polyacrylamide electrospinning spinning solution containing yeast;

[0033] 3) Spinning of electrospinning nanofibers with immobilized yeast: Slowly fill the electrospinning spinning solution in step 2 into a plastic syringe with a capacity of 10 mL, and use a self-made electrospinning device to adj...

Embodiment 2

[0036] 1) Preparation of electrospinning polymer or macromolecule mixed solution: weigh polyvinyl alcohol with a balance, under nitrogen protection, slowly add solvent water, magnetically stir for 30min, control the temperature at 100°C to mix evenly, and form a uniform solution , finally configured into a polyvinyl alcohol mixed solution with a mass concentration of 8wt%;

[0037] 2) Preparation of electrospinning spinning solution containing yeast: Weigh 0.1 g of yeast solid particles, add it to the mixed solution in step 1, and stir magnetically for 15 minutes to fully disperse the yeast cells in the mixed solution, and finally obtain Polyvinyl alcohol electrospinning spinning solution containing yeast;

[0038] 3) Spinning of electrospinning nanofiber immobilized yeast: Slowly fill the electrospinning spinning liquid in step 2 into a metal syringe with a capacity of 5mL, and use the purchased electrospinning device to adjust spinning parameters such as spinning Spinning w...

Embodiment 3

[0041] 1) Preparation of electrospinning polymer or macromolecule mixed solution: Weigh polylactic acid with a balance, under nitrogen protection, slowly add solvent dimethyl sulfoxide, magnetically stir for 300min, control the temperature at 45°C to make it evenly mixed, Form a homogeneous solution, and finally configure it into a polylactic acid mixed solution with a mass concentration of 11% by weight;

[0042] 2) Preparation of yeast-containing electrospinning spinning solution: Weigh 0.3 g of yeast solid particles, add it to the mixed solution in step 1, and magnetically stir for 35 minutes to finally obtain the yeast-containing electrospinning spinning solution;

[0043] 3) Spinning of electrospinning nanofibers with immobilized yeast: Slowly fill the electrospinning spinning solution in step 2 into a metal syringe with a capacity of 20 mL, and use a self-made electrospinning device to adjust spinning parameters such as spinning Spinning with a voltage of 12kV, a receivi...

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Abstract

The invention relates to beaded electrospun nanofiber immobilized saccharomycete and a preparation method thereof. Nanofiber comprises electrospun polymer or macromolecules, a solvent and a saccharomycete; prepared mixed solution is placed in an injector and is spun with an electrospinning instrument, microorganisms are introduced into the nanofiber by dispersion of the saccharomycete in a spinning solution, the advantages of the nanofiber such as high specific area, high porosity, large length-diameter ratio and bulkiness and porousness are combined, in-situ immobilization is achieved for saccharomycete cells, an active organic/microorganism composite is obtained, and a function similar to a multi-enzyme bioreactor is enabled. A product and preparation method of the invention have the advantages such as high processing efficiency, high reacting speed, ease of control, high antifouling capacity, continuous reusability, and ease of product separation. The beaded electrospun nanofiber immobilized saccharomycete is applicable to the fields such as biological catalysis, filtering and adsorption, drug delivery and tissue engineering.

Description

technical field [0001] The invention belongs to the field of microbial material science, in particular to the field of yeast immobilization, in particular to a beaded electrospun nanofiber immobilized yeast and a preparation method thereof. Background technique [0002] As a kind of cell microorganism, yeast has a complete enzyme catalytic system in the body, which has the characteristics of fast catalytic speed, high catalytic efficiency, and strong specificity. It plays a very important role in daily production practices, such as fermentation wine, biological Catalysis, filtration adsorption and drug delivery, etc. However, in the actual production practice, due to the harsh environmental conditions, the activity of yeast is greatly affected, which limits the practical application of yeast. The immobilization technology that emerged in the 1950s has effectively improved the above-mentioned problems, enabling yeast to maintain high activity, reducing production costs, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08D04H1/728D01D5/00D01D1/02
CPCC12N11/08D01D1/02D01D5/0076D04H1/728
Inventor 张青松郑林宝邓玲利穆齐峰高鲁杨超超刘鹏飞陈莉
Owner TIANJIN POLYTECHNIC UNIV
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