Fluorescent probe as well as preparation method, application and application method thereof

The technology of a fluorescent probe and an application method, which is applied in the detection field, can solve the problems of complex preparation process of the fluorescent probe, difficult mass production and preparation, high development cost, etc., and achieves low development cost, strong fluorescent luminescence characteristics, high biological phase and the like. capacitive effect

Active Publication Date: 2019-11-15
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a fluorescent probe, which aims to solve the problems of complex preparation process, high development cost, poor stability and difficulty in large-scale production of fluorescent probes in the prior art.

Method used

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  • Fluorescent probe as well as preparation method, application and application method thereof
  • Fluorescent probe as well as preparation method, application and application method thereof
  • Fluorescent probe as well as preparation method, application and application method thereof

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preparation example Construction

[0030] In an embodiment of the present invention, the preparation method of the fluorescent probe includes:

[0031] Dispersing boron powder in isopropanol to prepare a first dispersion with a concentration of 0.1-10 mg / mL;

[0032] subjecting the first dispersion to ultrasonic stripping of the probe to obtain a second dispersion;

[0033] The second dispersion liquid is subjected to a water-bath ultrasonic stripping treatment to obtain a third dispersion liquid;

[0034] The third dispersion liquid is subjected to fractional-speed centrifugation, and the resulting precipitate is made into boronene nanosheets, to obtain.

[0035] In a preferred embodiment of the present invention, the above-mentioned boron powder is dispersed in isopropanol to prepare the first dispersion liquid with a concentration of 0.1-10 mg / mL, which specifically includes:

[0036] The boron powder was dispersed in isopropanol to prepare a first dispersion with a concentration of 1 mg / mL.

[0037] In a...

Embodiment 1

[0054] The bulk boron powder was dispersed in isopropanol (IPA) to prepare a dispersion liquid with a concentration of 0.1 mg / mL, and then the dispersion liquid was subjected to ultrasonic peeling treatment of the probe (specific parameters: 900W ultrasonic power, total time 0.5 hours, ultrasonic 1s, pause for 5s, constant temperature at 5°C), and then perform ultrasonic peeling treatment in a water bath on the dispersion obtained after ultrasonication of the probe (specific parameters: 1200W ultrasonic power, total time of 1 hour, constant temperature at 5°C), and finally the stripped dispersion Perform split-speed centrifugation (specific parameters: centrifuge at 10,000 rpm for 10 minutes, take the upper layer liquid, then centrifuge at 13,000 rpm for 10 minutes, remove the sediment in the lower layer, and keep the temperature at 5°C) to obtain boronene nanosheets with uniform particle size and thin thickness.

Embodiment 2

[0056] Disperse the bulk boron powder in isopropanol (IPA) to prepare a dispersion liquid with a concentration of 10 mg / mL, and then subject the dispersion liquid to ultrasonic peeling treatment of the probe (specific parameters: 500W ultrasonic power, total time 2 hours, ultrasonic 5s , pause for 10s, constant temperature at 20°C), and then, the dispersion liquid obtained after the ultrasonic probe was subjected to water-bath ultrasonic stripping treatment (specific parameters: 500W ultrasonic power, total time 5 hours, constant temperature 20°C), and finally the stripped dispersion liquid Split speed centrifugation (specific parameters: centrifuge at 8000rpm for 20 minutes, take the upper liquid, then centrifuge at 11000rpm for 10 minutes, take the lower layer of sediment, keep the temperature at 15°C), and obtain boron nanosheets with uniform particle size and thin thickness.

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Abstract

The invention is applicable in the technical field of detection and provides a fluorescent probe as well as a preparation method, application and an application method thereof, wherein the fluorescentprobe is a two-dimensional boroxene nanosheet. The fluorescent probe is the two-dimensional boroxene nanosheet, takes on an obvious two-dimensional layered stacking structure, is about 20 nm in thickness, has strong fluorescence luminescence characteristic, is low in development cost, is simple and convenient in preparation process and is green and pollution-free. Through a test for performing cell biocompatibility on the boroxene nanosheet, the result indicates that even if at high concentration of 400ug/mL, a HeLa cell still can maintain the survival rate to be more than 80 percent on the boroxene nanosheet, and the boroxene nanosheet has high biocompatibility and is suitable for cell bioimaging.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a fluorescent probe and its preparation method, application and application method. Background technique [0002] Bioimaging is an important biotechnology that aims to develop new methods for in situ, real-time, and dynamic detection and analysis of specific molecular pathways in living cells or the human body. It is an important vector tool for studying the physiological functions of species and human diseases. The development of green, cheap, efficient and stable fluorescent probes is an important prerequisite for non-invasive research on biological processes in vivo at the molecular level. [0003] At present, common fluorescent probes can be mainly divided into three major systems: organic, inorganic and organic / inorganic hybrid. The most common fluorescent probes of inorganic materials can be mainly divided into graphene-based nanosheets / quantum dots, semicond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B35/02C09K11/63G01N21/64A61K49/00B82Y40/00
CPCC01B35/023C09K11/63G01N21/6458G01N21/6428A61K49/0019A61K49/0093B82Y40/00
Inventor 张晗马定涛赵劲来吴雷明谢建磊张豫鹏郭志男张家宜
Owner SHENZHEN UNIV
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