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Isoquinoline alkaloid type compound as well as preparation method and application thereof

A technology of isoquinoline alkaloids and compounds, which is applied in the prevention and treatment of tobacco black shank, in the field of isoquinoline alkaloid compounds, achieves the effects of easy industrial application, low production cost, and large biological yield

Active Publication Date: 2019-11-15
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention obtains a new isoquinoline alkaloid compound through the research and activity screening of the chemical constituents of Eurasian tang pine grass. The compound has not been reported so far. It is worth mentioning that the compound has significant The role of anti-tobacco black leg virus activity

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  • Isoquinoline alkaloid type compound as well as preparation method and application thereof
  • Isoquinoline alkaloid type compound as well as preparation method and application thereof
  • Isoquinoline alkaloid type compound as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Preparation of isoquinoline alkaloid compound C 12 h 11 NO 2 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0040] (1) Extraction of extract: Take the whole plant of Pinegrass tangensis, dry it in the sun, and crush it to 35-60 mesh. Weigh 3.5-5.2kg of crushed samples, place them in a 20L glass reactor, add 8-12L of 95% ethanol, reflux for 35-60min, filter out the extract; add 95% ethanol 8 to the filter residue again ~12L, reflux extraction for 35~60min, filter the extract. The two extracts were combined and concentrated to a small volume, then diluted with 4-6L of 3% tartaric acid solution, and then extracted twice with 4-6L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice again with 4-6 L of ethyl acetate, the extracted ethyl acetate phases were combined, and concentrated under reduced pr...

Embodiment 2

[0045] The samples of Eurasian pine grass came from Dali, Yunnan. Take the whole plant of Pinus japonica, dry it in the sun, and crush it to about 45 meshes. Weigh 3.8kg of crushed sample, place it in a 20L glass reaction kettle, add 8L of 95% ethanol, reflux for extraction for 38min, and filter out the extract; add 8L of 95% ethanol to the filter residue again, reflux for 40min, filter In addition to the extract, the two extracts were combined and concentrated to a small volume. Then it was diluted with 6L of 3% tartaric acid solution, and extracted twice with 6L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice again with 6 L of ethyl acetate, and the extracted ethyl acetate phases were combined and concentrated under reduced pressure to obtain 86.4 g of alkaloid part extract. The extract is mixed with 120g (80-100 mesh) thick silica gel, dried, and 280g silica gel (150-200 mesh) column chromatography, chloroform...

Embodiment 3

[0047] The samples of Eurasian pine grass came from Chuxiong, Yunnan. Take the whole plant of Pinus japonica, dry it in the sun, and crush it to 55 mesh. Weigh 4.6kg of crushed sample, put it in a 20L glass reaction kettle, add 12L of 95% ethanol, reflux for extraction for 25min, and filter the extract; add 12L of 95% ethanol to the filter residue again, reflux for 30min, filter In addition to the extract, the two extracts were combined and concentrated to a small volume, then diluted with 10 L of 3% tartaric acid solution, and extracted twice with 10 L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice again with 10 L of ethyl acetate, and the extracted ethyl acetate phases were combined and concentrated under reduced pressure to obtain 116.8 g of alkaloid part extract. The extract is mixed with 200g (80-100 mesh) thick silica gel, dried, and 380g silica gel (150-200 mesh) column chromatography, chloroform: acetone ...

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Abstract

The invention discloses an isoquinoline alkaloid type compound as well as a preparation method and application thereof. The compound is prepared from complete stools of thalictrum minus as a raw material through ethyl acetate extraction, silica gel column chromatography and high performance liquid chromatography separation and purification. The compound has a molecular formula C12H11NO2, and has astructural formula shown in the specification, and the compound is named as 1-(6-ydroxy-7-methyl isoquinoline-1-yl) aceton. The compound has a tobacco phytophthora inhibition function which is remarkably prior to that of agricultural streptomycin, and has a tobacco black shank prevention and treatment effect up to (72.2+ / -3.3)%. The compound is wide in production raw material distribution, largein biological amount, high in alkaloid content, simple in compound preparation process, low in production cost and easy in industrial application.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to an isoquinoline alkaloid compound obtained by extracting and separating for the first time from Eurasian tang pine grass. At the same time, the invention also relates to the preparation method of the compound and the application of the compound in the prevention and treatment of tobacco black shank. Background technique [0002] Thalictrum L. (Latin scientific name: Thalictrum L.) belongs to the perennial herb of Ranunculaceae. There are about 200 species of this genus in the world, among which there are about 67 species in China, most of which are distributed in the southwest. Among the plants of the genus Echinacea all over the world, there are more than 90 species of this genus with reported chemical components, and the main active components include alkaloids, saponins, flavonoids and other types. More than 43 species of plants in this genus have folk medi...

Claims

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Application Information

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IPC IPC(8): C07D217/16A01N43/42A01P3/00
CPCC07D217/16A01N43/42Y02A50/30
Inventor 曾婉俐高茜杨光宇李雪梅李晶黄海涛王晋许永宋春满孔维松刘欣胡秋芬
Owner CHINA TOBACCO YUNNAN IND