Cell preservation liquid capable of realizing long term effective preservation of cells

A preservation solution and long-term technology, applied in the field of medical biology, can solve problems such as gaps in actual needs, and achieve the effects of less activity loss, good preservation effect, and rapid bactericidal effect

Pending Publication Date: 2019-11-19
深圳市华晨阳科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are some cell preservation solutions on the market, but the storage time of cells in these preservation solution...

Method used

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  • Cell preservation liquid capable of realizing long term effective preservation of cells
  • Cell preservation liquid capable of realizing long term effective preservation of cells
  • Cell preservation liquid capable of realizing long term effective preservation of cells

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Experimental program
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Embodiment Construction

[0029] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0030] 1. Composition of Cell Preservation Solution

[0031] Disodium hydrogen phosphate 0.1-3g, potassium dihydrogen phosphate 0.1-4.1g, potassium chloride 0.01-2.2g, sodium chloride 0.6-1g, calcium chloride 0.001-0.01g, glacial acetic acid 0.1-7g, ethylenediaminetetra Acetic acid 1-16g, Tris 0.001-0.003g, sucrose 0.1-0.24g, surfactant 0.98-2.6g, deionized water to 100mL, pH 6.8-7.6.

[0032] Surfactant: It is a non-ionic detergent, which can be Tween 20 or sodium lauryl sulfate.

[0033] 2. Preparation of cell preservation solution

[0034] Accurately weigh each reagent according to the formula in the table below, then dissolve the reagent with a small amount of deionized water, then dilute to 100mL with deionized water, shake well, and finally adjust the pH of the solution to prepare the cell preservation solution.

[0035]

[0036]

[0037] In the cell pr...

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Abstract

The invention discloses a cell preservation liquid capable of realizing long term effective preservation of cells. The cell preservation liquid capable of realizing long term effective preservation ofcells comprises following components, disodium hydrogen phosphate 0.1-3g, potassium dihydrogen phosphate 0.1-4.1g, potassium chloride 0.01-2.2g, sodium chloride 0.6-1g, calcium chloride 0.001-0 0.01g, glacial acetic acid 0.1-7g, ethylene diamine tetraacetic acid 1-16g, trihytdroxy methyl-aminomethane 0.001-0.003g, sucrose 0.1-0.24g, and a surfactant 0.98-2.6g; the cell preservation liquid is diluted to 100mL with deionized water, and pH value is controlled to be 6.8 to 7.6. The beneficial effects are that: the cell preservation liquid is capable of maintaining cell activity in a relatively long term, so that long time effective preservation of cells is realized; when the cell preservation liquid is used in preservation of cells, in long preservation term, the cell preservation liquid is still capable of inhibiting breeding of microorganisms, and protecting cells from damage by microorganisms.

Description

technical field [0001] The invention relates to a cell preservation solution, in particular to a cell preservation solution capable of effectively preserving cells for a long time, and belongs to the field of medical biotechnology. Background technique [0002] Cell is the most basic unit of life and has application value in many aspects. For cells and cell products, the difference from ordinary biological products and pharmaceuticals is that cell activity needs to be maintained, and maintaining cell activity is a great challenge. [0003] Physiological saline is safe and convenient in clinical application, but the activity of cells decreases very quickly in saline, and the activity of cells loses as much as 30% after being stored in saline for 10 hours. It can be seen that normal saline is only suitable for short-term storage of cells. [0004] At present, there are some cell preservation solutions on the market, but the storage time of cells in these preservation solutio...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 巩赞华巩赞博巩赞斌张金银黄咏华
Owner 深圳市华晨阳科技有限公司
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