Method for separating and purifying cannabidiol (CBD) by using industrial hemp as raw material

A technology of industrial hemp and cannabidiol, applied in the field of medicine and chemical industry, can solve the problems of complex phenolic components and affecting product safety

Pending Publication Date: 2019-11-19
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the phenolic components in plant cannabis are complex, and it is easy to extract components with similar polarities, which affects product safety.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Extraction: Take more than 1 / 3 of the leaves from the upper end of the industrial hemp plant, put them into the tunnel-type ultrasonic and heating extraction equipment, use absolute ethanol as the solvent, the ratio of solid to liquid is 1:4, the ultrasonic power is 5000W, and the extraction temperature is 80°C. The extraction time is 3 hours, and the industrial hemp CBD extract is obtained.

[0017] 2. Concentration: Put the extract into a double-effect ascending membrane concentration tank for concentration, vacuum degree 0.075MaP, temperature 95°C, concentrate to 1 / 20 of the volume of the extract to obtain CBD concentrate.

[0018] 3. Settling: Add the concentrated CBD solution into the mixing tank, add 30 times of water, and stir for 40 minutes, and the speed of the stirring blade is 30 rpm. After standing still for 24 hours, the supernatant was removed to obtain the CBD precipitation solution.

[0019] 4. Primary chromatography: The diameter-to-length ratio of ...

Embodiment 2

[0025] 1. Extraction: Take more than 1 / 3 of the leaves from the upper end of the industrial hemp plant, put them into the tunnel-type ultrasonic and heating extraction equipment, use methanol as the solvent, the ratio of solid to liquid is 1:5, the ultrasonic power is 3000W, the extraction temperature is 95°C, and the extraction time is In 2 hours, the industrial hemp CBD extract was obtained.

[0026] 2. Concentration: Put the extract into a double-effect ascending membrane concentration tank for concentration, vacuum degree 0.075MaP, temperature 95°C, concentrate to 1 / 16 of the volume of the extract to obtain CBD concentrate.

[0027] 3. Settling: Add CBD concentrate and put it into a stirring tank, add 60 times of water, and stir for 30 minutes, and the speed of the stirring blade is 40 rpm. After standing still for 24 hours, the supernatant was removed to obtain the CBD precipitation solution.

[0028] 4. Primary chromatography: The diameter-to-length ratio of the chromat...

Embodiment 3

[0034] 1. Extraction: take more than 1 / 3 of the leaves of the industrial hemp plant, put them into a tunnel-type ultrasonic and heating extraction equipment, use absolute ethanol and methanol as solvents, the ratio of solid to liquid is 1:6, the ultrasonic power is 4000W, and the extraction temperature is 90 ℃, the extraction time is 3 hours, and the CBD extract of industrial hemp is obtained.

[0035] 2. Concentration: Put the extract into a double-effect ascending membrane concentration tank for concentration, vacuum degree 0.075MaP, temperature 95°C, concentrate to 1 / 20 of the volume of the extract to obtain CBD concentrate.

[0036] 3. Settling: Add CBD concentrate and put it into a stirring tank, add 50 times of water, and stir for 40 minutes, and the speed of the stirring blade is 30 rpm. After standing still for 20 hours, the supernatant was removed to obtain a CBD precipitation solution.

[0037] 4. Primary chromatography: The diameter-to-length ratio of the chromatog...

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Abstract

The invention relates to a method for separating and purifying cannabidiol (CBD) by using industrial hemp as a raw material. The method comprises: using more than 1/3 of the leaves on the upper ends of industrial hemp plants as raw materials, obtaining an extracting solution by combining tunnel type ultrasonic wave and heating, concentrating in a double-effect rising film concentration tank, settling in a stirring tank, removing the supernatant, carrying out macroporous resin LSA-12 primary chromatography on the precipitation liquid, concentrating, carrying out symmetrical spherical silica gelsecondary chromatography, concentrating, carrying out preparative high-performance liquid phase chromatography, concentrating the eluent, and crystallizing to obtain the CBD with a purity of 90-99.9%. Compared with the method in the prior art, the method of the present invention has characteristics of high CBD purity, high yield, simple process, low cost and easy industrialization.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and in particular relates to a method for separating and purifying cannabidiol (CBD) from industrial hemp. Background technique [0002] Cannabis (Cannabis sativa L.) is an annual herb of the Cannabis genus, which is distributed all over the world. Hemp seeds are rich in high-quality protein and unsaturated fatty acids, which can be used as food, health products and feed, and the active substances in its flowers and leaves are widely used in the fields of medicine and cosmetics. Cannabinoids are secondary metabolites containing alkyl and monoterpene groups unique to the cannabis plant. There are more than 70 cannabinoids isolated from the cannabis plant, mainly including THC (tetrahydrocannabinol), CBD (cannabidiol), CBC (cannabichromene), CBN (cannabinol), CBG (cannabigerol) And its propyl homologues, etc., among which THC and CBD are isomers of each other, with the highest conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/68C07C37/82C07C39/23
CPCC07C37/685C07C37/82C07C2601/16C07C39/23
Inventor 王振宇张华张胜宇李杰辛超
Owner HARBIN INST OF TECH
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