Sisal hemp-plant leaf cream production method

A production method and sisal technology are applied in the production field of sisal hemp paste, which can solve the problems of large floor area, long fermentation cycle, and low saponin content, and achieve the solution of large floor area, increased saponin content, and rate-enhancing effect

Inactive Publication Date: 2017-02-22
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main disadvantages of this method are: large flo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Raw material preparation: Squeeze the fresh sisal slag left after processing and extracting fibers, separate the slag from water, and obtain hemp juice for later use;

[0023] 2. Strain acclimatization: inoculate the Lactobacillus delbrueckii and Candida ethanolosa preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:1, respectively). 8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), each transfer volume was 10%, stirring for 5min every 12h , 30-35°C, cultured for 4 days.

[0024] The components of the Lactobacillus delbrueckii domestication culture solution: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diammonium citrate, 1.0g Tween80, K 2 HPO 4 2.0g, MnSO 4 ·H 2 O 0.05g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.

[0025] The components of the Candida ethanol acclimation culture me...

Embodiment 2

[0030] 1. Raw material preparation: Squeeze the fresh sisal slag left after processing and extracting fibers, separate the slag from water, and obtain hemp juice for later use;

[0031] 2. Strain domestication: inoculate the Lactobacillus delbrueckii preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), transfer 8% each time, stir for 5min every 12h, 30-35℃, Culture 4d.

[0032] The components of the domestication culture solution: 8.5g casein peptone, 11.5g beef extract, 5.5g yeast powder, 5.0g glucose, 5.0g sodium acetate, 1.8g diammonium citrate, 1.0g Tween80, K 2 HPO 4 2.4g, MnSO 4 ·H 2 O 0.04g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.

[0033] The components of the Candida ethanol acclimation culture solution: 9.0g peptone, 4.0g beef extract, 2.5g yeast powder, 2.5g glucose, 4.0g xylose, K 2 ...

Embodiment 3

[0038] 1. Raw materials: Squeeze the fresh sisal left after processing and extracting fibers, separate the slag and water, and obtain hemp juice for later use;

[0039] 2. Strain domestication: inoculate the Lactobacillus delbrueckii preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), the amount of each transfer is 12%, stirring for 5min every 12h, 30-35℃, Culture 2d.

[0040] The components of the domestication culture solution: 11.5g of casein peptone, 8.5g of beef extract, 4.0g of yeast powder, 4.0g of glucose, 4.0g of sodium acetate, 2.2g of diammonium citrate, 1.0g of Tween80, K 2 HPO 41.6g, MnSO 4 ·H 2 O 0.06g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.

[0041] The components of the Candida ethanol acclimation culture medium: 7.0g peptone, 2.2g beef extract, 1.5g yeast powder, 1.5g glucose, 2...

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Abstract

The invention relates to a sisal hemp-plant leaf cream production method. According to the production method, sisal hemp-plant leaves are produced into fibers to obtain sisal hemp-plant leaf juice, the juice is fermented, sediments are collected, leuconostoc mesenteroides, acclimated ethyl alcohol candida and lactobacillus delbrueckii are mixed to prepare fermentation inoculants for fermenting, acclimation is performed by the aid of a gradual acclimation method, the proportion of sisal hemp-plant leaf juice in acclimation culture solution is gradually increased, and the ethyl alcohol candida and the lactobacillus delbrueckii are respectively and gradually inoculated into high-proportion culture solution from low-proportion culture solution to obtain the acclimated ethyl alcohol candida and the acclimated lactobacillus delbrueckii. According to the method, a fermentation period is shortened to 4-5 days, yield of the sisal hemp-plant leaf cream is greatly improved, diosgenin content is improved by 20-30%, and difficulties further extracting diosgenin are reduced. The method changes a traditional production mode and solves the problem that a traditional method is large in occupied area, long in settlement period, low in diosgenin content and the like.

Description

technical field [0001] The invention relates to a sisal processing technology, in particular to a production method of sisal ointment. Background technique [0002] Sisal, also known as sisal hemp, water silk hemp, agave hemp, etc., belongs to the genus Agave and contains sisal saponin in the form of steroidal saponins, which can be made into steroids hormone drugs. [0003] The method of the raw material sesame paste (dry slag) of preparing saponin from sisal slag at present mainly is: [0004] 1) Material preparation: After fresh sisal produces fibers, the slag is pressed into water, and then the slag and water are separated to obtain hemp juice; [0005] 2) Fermentation in the pool: Put the sesame juice into the pool, ferment naturally for 15 to 30 days, collect the sediment and dry it in the sun to obtain sisal hemp paste. [0006] The main defects of this method are: large floor area, long fermentation cycle, low saponin content and the like. Contents of the invent...

Claims

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Application Information

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IPC IPC(8): A61K36/88
CPCA61K36/88A61K2236/19
Inventor 朱作华彭源德谢纯良龚文兵李智敏严理胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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