Sisal hemp-plant leaf cream production method
A production method and sisal technology are applied in the production field of sisal hemp paste, which can solve the problems of large floor area, long fermentation cycle, and low saponin content, and achieve the solution of large floor area, increased saponin content, and rate-enhancing effect
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Embodiment 1
[0022] 1. Raw material preparation: Squeeze the fresh sisal slag left after processing and extracting fibers, separate the slag from water, and obtain hemp juice for later use;
[0023] 2. Strain acclimatization: inoculate the Lactobacillus delbrueckii and Candida ethanolosa preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:1, respectively). 8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), each transfer volume was 10%, stirring for 5min every 12h , 30-35°C, cultured for 4 days.
[0024] The components of the Lactobacillus delbrueckii domestication culture solution: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diammonium citrate, 1.0g Tween80, K 2 HPO 4 2.0g, MnSO 4 ·H 2 O 0.05g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.
[0025] The components of the Candida ethanol acclimation culture me...
Embodiment 2
[0030] 1. Raw material preparation: Squeeze the fresh sisal slag left after processing and extracting fibers, separate the slag from water, and obtain hemp juice for later use;
[0031] 2. Strain domestication: inoculate the Lactobacillus delbrueckii preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), transfer 8% each time, stir for 5min every 12h, 30-35℃, Culture 4d.
[0032] The components of the domestication culture solution: 8.5g casein peptone, 11.5g beef extract, 5.5g yeast powder, 5.0g glucose, 5.0g sodium acetate, 1.8g diammonium citrate, 1.0g Tween80, K 2 HPO 4 2.4g, MnSO 4 ·H 2 O 0.04g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.
[0033] The components of the Candida ethanol acclimation culture solution: 9.0g peptone, 4.0g beef extract, 2.5g yeast powder, 2.5g glucose, 4.0g xylose, K 2 ...
Embodiment 3
[0038] 1. Raw materials: Squeeze the fresh sisal left after processing and extracting fibers, separate the slag and water, and obtain hemp juice for later use;
[0039] 2. Strain domestication: inoculate the Lactobacillus delbrueckii preserved in the laboratory into the domestication culture medium (sterilized hemp juice and domestication culture medium at mass ratios of 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 in sequence), the amount of each transfer is 12%, stirring for 5min every 12h, 30-35℃, Culture 2d.
[0040] The components of the domestication culture solution: 11.5g of casein peptone, 8.5g of beef extract, 4.0g of yeast powder, 4.0g of glucose, 4.0g of sodium acetate, 2.2g of diammonium citrate, 1.0g of Tween80, K 2 HPO 41.6g, MnSO 4 ·H 2 O 0.06g, distilled water 1.0L, pH 6.8, sterilized at 115°C for 20min.
[0041] The components of the Candida ethanol acclimation culture medium: 7.0g peptone, 2.2g beef extract, 1.5g yeast powder, 1.5g glucose, 2...
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