Modified biopolymer and use thereof in 3D printing

A biopolymer and modification technology, applied in biochemical equipment and methods, microorganisms, 3D culture, etc., can solve the problem of reducing biological activity, low ink viscosity, and the inability to simultaneously solve the problem of good and accurate printing of biopolymer-based bioinks. Responsibility and other issues, to achieve the effect of high biological activity, stable performance, good viscosity and self-coagulation characteristics

Inactive Publication Date: 2019-11-22
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to overcome the said deficiency of prior art, provide a kind of modified biopolymer and preparation method thereof, to solve the ink viscosity that existing biopolymer is used as bio-ink base material to cause very low and is not suitable for accurate Technical issues in the construction of three-dimensional structures and the reduction of biological activity during cross-linking
[0014] Another object of the present invention is to provide a modified biopolymer bioink and its application to overcome the problem that the existing biopolymer-based ink cannot simultaneously solve the problems of good and accurate printing and subsequent good biological activity of the biopolymer-based bioink technical problem

Method used

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  • Modified biopolymer and use thereof in 3D printing
  • Modified biopolymer and use thereof in 3D printing
  • Modified biopolymer and use thereof in 3D printing

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preparation example Construction

[0043] Correspondingly, the embodiment of the present invention also provides a preparation method of the above-mentioned modified biopolymer. The technical process of the preparation method of the modified biopolymer is as figure 1 As shown, it includes the following steps:

[0044] S01: Carry out ureidation reaction between ureidopyrimidone containing isocyanate group and biopolymer body containing amine group and / or carboxyl group in the first reaction solvent to generate ureidopyrimidone grafted biopolymer ;

[0045] S02: Condensing the biopolymer grafted with ureidopyrimidone and a compound containing tyramide groups in a second reaction solvent containing a catalyst to obtain a biopolymer modified with ureidopyrimidone groups and tyramide groups Modified biopolymers.

[0046] Wherein, the ureidopyrimidinone containing isocyanate group in the step S01 and the biopolymer body are carried out in the first reaction solvent for ureidation reaction formula as follows figu...

Embodiment 11

[0070] This embodiment provides a modified biopolymer and a preparation method thereof. The modified biopolymer comprises a gelatin backbone onto which ureidopyrimidone groups and tyramine groups are grafted.

[0071] The preparation method of the modified biopolymer comprises the following steps:

[0072] Step S11: Under nitrogen protection, 6 g of gelatin was dissolved in 100 mL of dimethyl sulfoxide at 55 °C by magnetic stirring, and then cooled to room temperature; 0.3 g of 2(6-isocyanatohexylaminocarbonylamino)- 6-Methyl-4[1H]pyridone was added to the gelatin solution and reacted at room temperature for 24 hours; the reacted solution was precipitated 3 times through 1 L of ethanol solution, and then vacuum-dried for 24 hours to obtain 5.1 g of light yellow fixed , which is to generate ureidopyrimidinone-grafted gelatin, and its yield is calculated to be 85%;

[0073] Step S12: Weigh 1.5 g of the light yellow solid obtained in Step S11 and dissolve it in 100 mL of deioni...

Embodiment 12

[0076] This embodiment provides a modified biopolymer and a preparation method thereof. The modified biopolymer comprises a gelatin backbone onto which ureidopyrimidone groups and tyramine groups are grafted.

[0077] The preparation method of the modified biopolymer comprises the following steps:

[0078] Step S11: Under nitrogen protection, 6 g of gelatin was dissolved in 100 mL of dimethyl sulfoxide at 55 °C by magnetic stirring, and then cooled to room temperature; 0.15 g of 2(6-isocyanatohexylaminocarbonylamino)- 6-Methyl-4[1H]pyridone was added to the gelatin solution and reacted at room temperature for 24 hours; the reacted solution was precipitated 3 times through 1 L of ethanol solution, and then vacuum-dried for 24 hours to obtain 5.1 g of light yellow fixed , which is to generate ureidopyrimidinone-grafted gelatin, and its yield is calculated to be 85%;

[0079] Step S12: Weigh 1.5 g of the light yellow solid obtained in Step S11 and dissolve it in 100 mL of deion...

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Abstract

The invention discloses a modified biopolymer, a preparation method of the modified biopolymer, modified biopolymer bio-ink and application thereof. The modified biopolymer comprises a biopolymer mainchain, and a ureido pyrimidinone group and a tyramine group are grafted on the biopolymer main chain. The modified biopolymer bio-ink comprises a solvent and a modified biopolymer dissolved in the solvent, and further comprises a biological functional component loaded by the modified biopolymer, with the modified biopolymer in the bio-ink being the modified biopolymer provided by the invention. The modified biopolymer has good viscosity, has the characteristic that the viscosity is adjustable along with the temperature, and also has the characteristic that self-solidification is directly carried out at room temperature, and gel formed by solidification has good mechanical properties. The modified biopolymer bio-ink has good viscosity and biocompatibility, is especially suitable for 3D printing, can be used for direct printing of a biological scaffold, and avoids additional addition of components harmful to biological components such as a cross-linking agent and the like.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a modified biopolymer, a preparation method thereof, bioink and its application. Background technique [0002] On the basis of 3D printing technology, 3D bioprinting has great development potential in the fields of preparing artificial organs and tissues, realizing personalized treatment and drug screening by loading cells and growth factors. As the most widely used bioprinting method, micro-extrusion printing is one of the most important problems limiting its rapid development and clinical application is the lack of bioinks with both excellent printing performance and bioactivity. Generally, bioinks for 3D bioprinting need to have the following properties: [0003] a). Biological activity: ensure that the cells have a high survival rate, good spreading, proliferation and differentiation capabilities; [0004] b). Rapid prototyping capability: rapid prototyping...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08B37/08B33Y70/00B33Y80/00C12N5/00
CPCB33Y70/00B33Y80/00C08B37/003C08B37/0072C08H1/00C12N5/0062C12N2513/00C12N2533/30
Inventor 阮长顺何慧敏林子锋吴明明李铎
Owner SHENZHEN INST OF ADVANCED TECH
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