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Bacillus subtilis JZ2-1-12 and application thereof

A technology of Bacillus subtilis and microbial strains, applied in the field of microorganisms, to achieve good thermal stability, acid-base stability, and strong inhibitory activity

Active Publication Date: 2019-11-22
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are more and more reports about biocontrol bacteria inhibiting plant pathogens, but there are few reports about biocontrol bacteria inhibiting the activity of potato dry rot pathogens and potato virus Y

Method used

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  • Bacillus subtilis JZ2-1-12 and application thereof
  • Bacillus subtilis JZ2-1-12 and application thereof
  • Bacillus subtilis JZ2-1-12 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The isolation of embodiment 1 bacterial strain JZ2-1-12

[0025] The distiller's grain samples were collected from the wine cellar of Qinghai Huzhu Highland Barley Distillery. Take 5 g of distiller's grains sample, put it in 100 ml of sterile water, shake it fully at room temperature at 200 r / min for 30 minutes, and obtain the original liquid of distiller's grains sample. Draw 1mL of sample stock solution and add it to 9mL sterile water to make 10 -1 The solution. And so on, dilute step by step to 10 -2 、10 -3 . Take 0.2 mL of the solution and spread it on the plate of beef extract-peptone medium (beef extract 3g, peptone 10g, Nacl 5g, agar 20g, distilled water 1000mL, pH 7.0-7.2). Place the petri dish in an incubator at 28°C for a certain period of time and observe. When colonies appear on the medium plate, pick a small amount of hyphae or spores and inoculate them on a new medium plate until a single colony appears on the medium plate, then store the strains on ...

Embodiment 2

[0026] Identification of embodiment 2 bacterial strain JZ2-1-12

[0027] 1) Morphological identification of strain JZ2-1-12

[0028] Take the activated bacterial strain JZ2-1-12, and observe the colony morphological characteristics (including shape, size, luster, raised shape, transparency, edge, etc.) ).

[0029] Such as figure 1 As shown, the strain JZ2-1-12 was purple by Gram staining, and it was a Gram-positive bacterium. The cells were straight rods and moved with perinatal flagella. The colonies are neatly bordered, milky white, opaque, flat, and wrinkled on the surface.

[0030] 2) Physiological and biochemical identification of strain JZ2-1-12

[0031] Refer to the relevant content of the fourteenth chapter of biochemical characteristics determination in "Bergey's Bacteria Identification Manual" (Ninth Edition), carry out citrate utilization test, contact enzyme, glucose oxidative fermentation, sugar and alcohol on strain JZ2-1-12 Fermentation, methyl red (M.R), V...

Embodiment 3

[0049] Teratogenicity of the fermented liquid extract in embodiment 3 to the mycelium of the potato dry rot pathogenic fungus Mycelium of the potato dry rot pathogenic fungus hypha adjacent to the edge of the bacteriostatic zone and normal state were picked respectively in embodiment 2 and made slide specimens, each Each treatment was repeated three times, and the hyphal morphology of the treatment group and the control group were observed under an optical microscope.

[0050] Such as image 3 It was shown that both the extracts of the two fermentation broths had strong teratogenic effects on the mycelium of the potato dry rot pathogen. In normal state, the hyphae of the pathogenic fungus of potato dry rot were uniform in thickness, smooth in surface, plump and extended to the surroundings and grew normally. The hyphae adjacent to the edge of the inhibition zone treated with n-butanol extract were twisted and folded, and mycelium bifurcated, broken and uneven in thickness. T...

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Abstract

The invention discloses bacillus subtilis JQ2.11-12 and application thereof. The bacillus subtilis JQ2.11-12 is preserved in the Guangdong Microbial Culture Collection Center on June 17 th, 2019, andthe preservation number is GDMCC No:6096. The strain is separated from distilled grains of highland barley baijiu, and it is proved through experiments that a fermentation product of the strain can effectively inhibit potato dry rot pathogens and potato viruses Y, has a potential and application prospect as a biocontrol bacterium preparation for preventing and controlling the potato dry rot and potato viruses Y.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus subtilis JZ2-1-12 and an application thereof. Background technique [0002] Potato dry rot is a disease caused by Fusarium solani infecting tubers, and its pathogenic species has 9 species and varieties. Infested tubers heal slowly and the diseased site will expand further in a lower temperature and higher humidity environment. In addition, although potatoes have high disease resistance during harvest, they are vulnerable to disease infection if there are many wounds. Potato dry rot is very common in various production areas in my country, and the occurrence of dry rot has seriously reduced the yield of potatoes in my country. Usually, the potato yield loss caused by dry rot during potato cellaring is 6-25%, and it is as high as 60% in severe cases. It can be seen that the prevention and treatment of potato dry rot, especially biological control, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 沈硕李玮王舰
Owner QINGHAI UNIVERSITY
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