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Penicillium oxalicum capable of degrading organic acid smelly matter and scale-up cultivation method and application of Penicillium oxalicum

A technology of Penicillium oxalate and enlarged culture, applied in the field of Penicillium oxalate and its enlarged culture, can solve the problems of limited deodorization effect of livestock manure and the like, and achieve the effects of easy growth, strong growth adaptability and high tolerance

Active Publication Date: 2019-11-26
NANPING JIANYANG DISTRICT JIXIANG ANIMAL HUSBANDRY CO LTD
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Problems solved by technology

But the deodorizing effect of existing bacterial strains to poultry manure is still limited, therefore, the applicant has been committed to screening a microorganism with better deodorizing effect to poultry manure for a long time

Method used

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  • Penicillium oxalicum capable of degrading organic acid smelly matter and scale-up cultivation method and application of Penicillium oxalicum
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  • Penicillium oxalicum capable of degrading organic acid smelly matter and scale-up cultivation method and application of Penicillium oxalicum

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Embodiment Construction

[0021] The technical solution of the present invention will be described in detail below in conjunction with the embodiments.

[0022] A Penicillium oxalicum that degrades organic acid and odor, which is Penicillium oxalicum DH-1, was deposited in the China Center for Type Culture Collection on April 16, 2018, and the preservation number is CCTCC NO: M 2018201; Address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0023] Penicillium oxalicum DH-1 of the present invention is separated and screened from the accumulated dairy cow breeding manure, and the specific steps are as follows:

[0024] (1) Take 0.2 g of dairy cow manure accumulation and inoculate it into 100 mL of liquid screening medium. The configuration steps of the liquid screening medium are as follows: first prepare the basal medium, and the basal medium is based on glucose 20 g / L, tryptone 20 g / L, yeast extract 10g / L, dry cow manure 5 g / L, adjust the pH to 6.5~7.5, add water to the volume, a...

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Abstract

The present invention provides a Penicillium oxalicum capable of degrading organic acid smelly matter and a scale-up cultivation method and application of Penicillium oxalicum. The Penicillium oxalicum is Penicillium oxalicum DH-1, is preserved in China Typical Culture Collection Center on April 16, 2018, and has a preservation number of CCTCC NO:M 2018201, and the preservation address is No. 299,Bayi Road, Wuchang District, Wuhan City, Hubei Province. Samples are collected from stacked dairy cow manure, and are inoculated to a screened medium, a plurality of antibiotics, multiple organic acids, indole and skatole are added to the screened medium so as to perform induced habituated culture of bacteria in the samples, and separation and purification are performed so as to obtain the Penicillium oxalicum DH-1; and the Penicillium oxalicum has high growth adaptability, high tolerance to organic acids and antibiotics, can degrade organic acid smelly matter effectively, and is very suitable for cow manure treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganism application, and in particular relates to a penicillium oxalicum for degrading organic acid and odor, its enlarged cultivation method and its application. Background technique [0002] Fungi are an important part of natural ecosystems, and their unique cell structure and biochemical and physiological functions endow them with many excellent characteristics. Compared with bacteria, fungi have greater advantages in pollutant degradation, stress resistance, and environmental restoration. Among them, Penicillium has simple growth requirements and produces many secondary metabolites. It has been widely used in industrial enzyme production, biomedicine and other industries. It has strong adaptability and can use abundant and cheap raw materials or organic pollutants as Therefore, it has received more and more attention in the field of environmental pollution control in recent years....

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F11/02C02F103/20C12R1/80
CPCC12N1/14C02F11/02C02F2103/20C12R2001/80C12N1/145
Inventor 王宗寿施晞石神炳徐华锋潘建东许绍堂高宗耀魏发辉李振生谢忠卿
Owner NANPING JIANYANG DISTRICT JIXIANG ANIMAL HUSBANDRY CO LTD
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