Fluorescence analysis system constructed based on gold nanocluster AuNCs@CFP and used for specifically responding to p-nitrophenol
A technology of p-nitrophenol and gold nanoclusters, which is applied in the direction of material analysis, material analysis, fluorescence/phosphorescence, etc. through optical means, can solve time-consuming and complicated problems, and achieve simple preparation methods, easy synthesis, and fluorescent properties excellent effect
Pending Publication Date: 2019-12-03
HENAN NORMAL UNIV
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Most of these methods are relatively expensive, time-consuming, complicated and require large amounts of organic solvents
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[0019] Preparation of gold nanoclusters AuNCs@CFP
[0020] Take 0.5mL HAuCl 4 Put the solution (24.28mM) in a round bottom flask (50mL), add 8.28mL of pure water, stir while heating, add 1.22mL (5mM) cefoperazone sodium (CFP) after the solution is boiled, continue to react for 11h, cool to room temperature, use Filter with 0.45µm hydrophilic PTFE, and finally store the synthesized gold nanoclusters AuNCs@CFP in the refrigerator (4°C) for later use.
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The invention discloses a fluorescence analysis system constructed based on a gold nanocluster AuNCs@CFP and used for specifically responding to p-nitrophenol. 200 mu L of gold nanocluster AuNCs@CFP,500 mu L of PBS buffer solution with pH 10 and to-be-tested p-nitrophenol are taken to fix the volume to 4 mL, a reaction is performed at 45 DEG C for 50min, the fluorescence intensity is measured atan excitation wavelength of 334nm, and calculation is performed according to the measured fluorescence intensity in combination with a regression equation to obtain the concentration of the to-be-tested p-nitrophenol; and the linear concentration range of the p-nitrophenol is 1.25-40 mu M, the regression equation is F0 / Fi = 0.0258C + 1.003, F0 and Fi respectively represent the fluorescence intensity of a mixed system before and after the p-nitrophenol is added, a correlation coefficient is R2 = 0.9922, a detection limit LOD is 1.92 mu M, the concentration of the p-nitrophenol is 25 mu M, the measurement is repeated for 11 times, and the relative standard deviation RSD is 1.13%. In the fluorescence analysis system disclosed by the invention, the gold nanocluster AuNCs@CFP is simple in preparation method and is easy to synthesize, and the gold nanocluster AuNCs@CFP has excellent fluorescence performance.
Description
technical field [0001] The invention belongs to the technical field of construction of a fluorescence analysis system for specifically responding to p-nitrophenol, and specifically relates to a fluorescence analysis system for specifically responding to p-nitrophenol constructed on the basis of gold nanoclusters AuNCs@CFP. Background technique [0002] p-Nitrophenol (4-NP) is widely used in pesticide synthesis, dye processing, leather coloring, wood preservation, drug synthesis and explosives, etc. Despite its widespread use, this compound is known as a highly dangerous phenolic class of organic matter due to its carcinogenicity, mutagenicity, and skin disease-causing properties. Therefore, it is necessary to develop an effective and simple analytical method for the highly sensitive detection of p-nitrophenol. The reported detection methods, including high performance liquid chromatography, UV-visible spectrophotometry, capillary electrophoresis and fluorescence methods, we...
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CPCG01N21/643
Inventor 王治科张楠赵书南叶存玲范顺利
Owner HENAN NORMAL UNIV