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DSC electrode system capable of applying electric field

An electrode system and electrode technology, applied to the preservation of human or animal bodies, measuring devices, instruments, etc., can solve the problems of no unified method, increased difficulty in microscopic observation, and inconvenient real-time recording, etc., so as to inhibit low temperature damage and improve low temperature. The effect of saving efficiency

Inactive Publication Date: 2019-12-03
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The experimental results of microscopic observation are relatively intuitive and clear, but there are still three problems in the following aspects: first, the observation sample needs to be thin, which is inconsistent with the actual state of biological materials in practical applications; second, most of the salt ions and ice crystals They are all colorless, which adds difficulty to more detailed microscopic observation; third, the analysis of the results of microscopic observation often requires subjective identification, and there is no unified method, which is not convenient for more subtle quantitative calculations and in-depth mechanism analysis
[0006] The traditional method of measuring and verifying the effect of applied electric field on biological materials can only be further analyzed by comparing the ice crystal morphology of water in biological tissues after freezing or the different properties of biological materials after rewarming, which is not convenient for real-time recording

Method used

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  • DSC electrode system capable of applying electric field
  • DSC electrode system capable of applying electric field
  • DSC electrode system capable of applying electric field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Ultrapure water (hereinafter referred to as grade I water) filtered and purified by the Millipore Millipore Clear-D24UV pure water integrated system was used as the experimental sample, and the differential scanning calorimeter 1 was selected from the Q2000DSC analyzer of TA Company. The experimental steps are as follows:

[0063] 1.) Open the experimental cabin of the differential scanning calorimeter 1, place the reference crucible 5 and the microelectrode crucible 6 in the corresponding positions, connect the electrode lead 65 of the microelectrode crucible 6 with the signal generator 3, and set it up Signal parameters to be output;

[0064] 2.) Use a micro-injector with a specification of 2 μl to draw a total of 4 μl of grade I water twice into the electrode 63 in the microelectrode crucible 6 and cover the ceramic crucible cover 62. During the operation, ensure that the grade I water is completely injected between the gaps of the electrodes 63;

[0065] 3.) Set t...

Embodiment 2

[0071] Grade I water and NaCl powder with a purity of 99.99% from Alfa Aesar Company were used to configure physiological saline as the experimental samples, and the differential scanning calorimeter 1 was Q2000DSC analyzer from TA Company. The experimental steps are as follows:

[0072] 1.) Open the experimental cabin of the differential scanning calorimeter 1, place the reference crucible 5 and the microelectrode crucible 6 in the corresponding positions, connect the electrode lead 65 of the microelectrode crucible 6 with the signal generator 3, and set it up Signal parameters to be output;

[0073] 2.) Use a micro-injector with a specification of 2 μl to draw a total of 4 μl of physiological saline twice into the electrode 63 in the microelectrode crucible 6 and cover the ceramic crucible cover 62. During the operation, ensure that the physiological saline is completely injected into the electrode 63 between the gaps;

[0074] 3.) Set the temperature control program for t...

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Abstract

The invention discloses a DSC electrode system capable of applying an electric field. The DSC electrode system comprises a differential scanning calorimeter, a computer, a signal generator, a self-pressurization liquid nitrogen tank, and a reference crucible; the self-pressurization liquid nitrogen tank is connected with the differential scanning calorimeter, and used for controlling the temperature in real time; the differential scanning calorimeter is connected with the computer and used for transmitting a signal and recording an experiment result; the system further comprises a microelectrode crucible, the microelectrode crucible comprises a ceramic crucible, a ceramic crucible cover, a welding point, 2 electrodes and electrode lead lines which are fixed in the ceramic crucible; the gapis formed between the electrodes to store a tested sample; the welding points are remained on the upper ends of the electrodes, one end of each electrode lead line is connected with the welding point, and the other end is connected with the signal generator and used for applying electric field to the electrode. The invention further provides application of the DSC electrode system capable of applying electric field in the low-temperature storage of the biological and material field. The DSC electrode system can effectively inhibit the low-temperature damage of the biological material, and thelow-temperature storage efficiency of the biological material is improved.

Description

technical field [0001] The invention relates to the field of system design for observing factors and mechanisms affecting cryopreservation of biological materials, in particular to a DSC electrode system capable of applying an electric field. Background technique [0002] Biodielectrics are an important research direction in the interdisciplinary field of electromagnetism and biomaterials. The study of biodielectrics will help to deepen people's understanding of the interaction between electromagnetism and biomaterials, and promote its wider application. At present, an important problem faced by cryopreservation technology of biological materials is how to avoid low temperature damage to biological materials during the cooling process. Chemical damage from dehydration or physical damage from cell membranes being punctured by ice crystals. Therefore, avoiding low temperature damage to biological materials is mainly to inhibit the crystallization of water in biological tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/20
CPCG01N25/20A01N1/0294A01N1/0284A01N1/0242G01N25/4866A01N1/0252
Inventor 钟力生宋晓源高景晖于钦学刘琳何佳熹李亚飞
Owner XI AN JIAOTONG UNIV