Culture method of sinapine degradation bacteria capable of producing polyphenol oxidase

Abstract

The invention provides a culture method of sinapine degradation bacteria capable of producing polyphenol oxidase. The culture method of sinapine degradation bacteria capable of producing polyphenol oxidase comprises the following steps of (1) inoculating brain heart infusion agar culture mediums with sinapine degradation bacteria, and performing culturing under the condition of 25-30 DEG C for 12-96 hours; and (2) selecting single colonies from brain heart infusion agar recovery culture mediums, inoculating enzyme production culture mediums with the selected single colonies, and performing shaking culture in a shaking culture box at 25-30 DEG C for 4-6 days. According to the method disclosed by the invention, the culture condition is simple and controllable, and recovery and enzyme production effects are good; and the polyphenol oxidase secretion quantity of the sinapine degradation bacteria can be effectively stimulated, and the activity of the polyphenol oxidase can be notably increased, so that a technical reference is provided for further researching the enzymology property and the action mechanism of the sinapine degradation bacteria.

Description

technical field [0001] The invention relates to microbial degradation, in particular to a method for cultivating polyphenol oxidase produced by sinapine-degrading bacteria. Background technique [0002] Sinapine is one of the most important anti-nutritional factors in cruciferous plant feed resources such as rapeseed meal. It mainly has the following harmful effects: 1) Under the action of intestinal microorganisms, it is degraded into trimethylamine, Make animals produce fishy smell syndrome, affect animal health and reduce the quality of livestock products; 2) taste bitter, affect feed palatability and animal feed intake; 3) can combine with protein, trace elements, etc. to reduce their digestibility; 4) It has anti-androgen effect and affects animal reproductive performance. Sinapine-degrading bacteria refer to a class of microorganisms that have the function of degrading sinapine, and they can effectively degrade sinapine by using the biological enzymes secreted by them...

AI Technical Summary

Problems solved by technology

[0002] Sinapine is one of the most important anti-nutritional factors in cruciferous plant feed resources such as rapeseed meal. It mainly has the following harmful effects: 1) Under the action of intestinal microorganisms, it is degraded into trimethylamine, Make animals produce fishy smell syndrome, affect animal health and reduce the quality of livestock products; 2) taste bitter, affect feed palatability and animal feed intake; 3) can combine with protein, trace elements, etc. to reduce their digestibility; 4) It has anti-androgen effect and affects animal reproductive performance

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