Preparation and application of bacillus subtilis SL-19 microbial inoculum for biological prevention and treatment of alternaria alternata
A technology of Bacillus subtilis and SL-19, applied in the direction of biocide, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of good control effect of tobacco red spot disease, and achieve convenient technical process and low cost , strong inhibitory effect
Pending Publication Date: 2019-12-13
SHAANXI OF CHINA TOBACCO GENERAL CORP
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Problems solved by technology
[0006] The purpose of the present invention is to solve the problem of the lack of good control effect on tobacco red spot disease in the prior art, and propose a vertical greening device for interior decoration
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[0023] Preparation of Bacillus subtilis SL-19 bacterial agent for biological control of tobacco red spot disease. Bacillus subtilis SL-19 is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, with a preservation number of CGMCCNo.18089;
[0024] The preparation method comprises the following steps:
[0025] S1, slant-plane activation and expansion culture: inoculate the slant of the Bacillus subtilis SL-19 test tube on the flat plate for colony activation and amplification, inoculate the amplified colony plate in liquid medium for activation and culture until the logarithmic growth phase (60 hours), to obtain the activated flora liquid;
[0026] S2, seed liquid fermentation: the activated flora liquid obtained in step 1 is fermented and cultivated in the fermentation culture liquid inoculated in the seed tank with 9% of the volume of the seed tank, the dissolved oxygen is 18%, the rotating speed is 230rpm, and the temperature ...
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The invention discloses preparation and application of a bacillus subtilis SL-19 microbial inoculum for biological prevention and treatment of alternaria alternate, and belongs to the technical fieldof biological prevention and treatment; the microbial inoculum of the invention is prepared by the following steps: (1) slant activating and amplifying culture; (2) fermenting and culturing seed liquid; (3) fermenting and culturing, wherein the number of live spores in the final bacillus subtilis SL-19 microbial inoculum is more than or equal to 5 billion / ml; and when the slant is activated and the culture is amplified, inoculating the slant of test tube of the bacillus subtilis SL-19 to a plate for bacterial colony activation and amplification, and inoculating the amplified bacterial colony in a liquid culture medium for activation culture for a logarithmic phase (60 hours) to prepare activated microflora liquid. The preparation and application of the bacillus subtilis SL-19 microbial inoculum adopts a biological prevention and treatment mode of treating bacteria with bacteria, the bacillus subtilis SL-19 is used for the prevention and treatment of the alternaria alternate, and the bacillus subtilis SL-19 microbial inoculum is combined with a leaf surface spraying technology, so that the morbidity of the alternaria alternate is reduced to 4.8 percent, and the final prevention andtreatment effect can be as high as 83.78 percent.
Description
technical field [0001] The invention relates to the technical field of biological control, in particular to the preparation and application of a bacillus subtilis SL-19 bacterial agent used for the biological control of tobacco red spot disease. Background technique [0002] Tobacco red spot disease is an airborne epidemic disease that occurs widely and is widely distributed. It is a very serious and intractable fungal disease. The incidence rate of tobacco red spot disease in different years is 30% to 50%. In the later period of rainy and humid conditions, It can cause devastating disasters, seriously affect the production of cash crop tobacco, and according to statistics, will cause hundreds of millions of yuan in economic losses every year. At present, the disease occurs in all tobacco-producing areas in China, and it is serious in Shandong, Henan, Anhui, Heilongjiang, Jilin, Shaanxi and other places; followed by Sichuan, Yunnan, Guizhou, Liaoning and other tobacco-produc...
Claims
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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
CPCC12N1/20
Inventor 张家韬崔传斌安德荣董绘阳陈超耿伟李立丹王安然
Owner SHAANXI OF CHINA TOBACCO GENERAL CORP