Method for constructing, observing and analyzing tree shrew fusarium type keratitis model

An analysis method and Fusarium technology, applied in the field of Fusarium keratitis, can solve problems such as complex construction process, and achieve the effect of simple operation steps, easy operation, timely and effective observation and analysis

Inactive Publication Date: 2019-12-13
李妍
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] In view of the occurrence of Fusarium keratitis and the specific phenomenon of complications, it is necessary to construct a Fusarium keratitis model and further observe and analyze the construct

Method used

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Examples

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Embodiment 1

[0037] A method for constructing and observing and analyzing tree shrew Fusarium keratitis model, comprising the following steps:

[0038] S1. Fusarium culture:

[0039] The inoculation loop is sterilized by heating with an alcohol lamp. After the inoculation loop is cooled, use the inoculation loop to scrape an appropriate amount of Fusarium species, and inoculate it into the Sabouraud dextrose agar medium, and place the inoculated medium at 26°C Cultivate in a common incubator for 7d-14d;

[0040] S2, Fusarium spore preparation:

[0041] S2.1. Heat and sterilize the inoculation loop with an alcohol lamp. After the inoculation loop is cooled, use the inoculation loop to scrape all the hyphae cultured in the medium in step S1 into a centrifuge tube containing 5ml of normal saline;

[0042] S2.2. Use a pipette to draw 5 mL of physiological saline and repeatedly rinse the surface of the mycelia cultured in the medium in step S1 to obtain a fungal suspension, and collect the fu...

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Abstract

The invention discloses a method for constructing, observing and analyzing a tree shrew fusarium type keratitis model. The method comprises the following steps: fusarium culture, fusarium spore preparation, experiment grouping and fusarium type keratitis modeling, fusarium type keratitis model culture analysis and fusarium type keratitis observation. According to the invention, the eyes of the tree shrew are used as experimental eyes, and the construction and analysis observation of the fusarium type keratitis model are carried out, so that the construction and timely and effective observationand analysis of the fusarium type keratitis model can be realized, and the popularization and application are facilitated.

Description

technical field [0001] The invention relates to the technical field of Fusarium keratitis, in particular to a method for constructing and observing and analyzing a tree shrew Fusarium keratitis model. Background technique [0002] Fusarium keratitis is caused by fungal infection. The main manifestation of Fusarium keratitis is serpentine corneal ulcer. The ulcer surface is rough and rises above the corneal surface. Early ulcers are superficial, with punctate nodular infiltration on the surface, grayish white or milky white turbidity; the shape is irregular, the surface is rough, dry, and the boundary with the healthy cornea is clear. [0003] In view of the occurrence of Fusarium keratitis and the specific phenomenon of complications, it is necessary to construct a Fusarium keratitis model and further observe and analyze the constructed Fusarium keratitis model. The construction of the traditional Fusarium keratitis model The process is relatively complicated, and timely an...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12Q1/04C12R1/77
CPCC12N1/14C12N3/00C12Q1/04G01N2333/37
Inventor 李妍胡竹林孔蕾刘海戴敏
Owner 李妍
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