Celery seed extract and preparation method thereof

A celery seed extract and a celery seed technology are applied in the field of celery seed extract and its preparation, which can solve the problems involving less separation and purification, and achieve the effect of increasing the content of total flavonoids

Inactive Publication Date: 2019-12-20
杨凌萃健生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the total flavonoids of celery seeds mainly focuses on the extraction of flavonoids, the research on pharmacological activities, etc., but seldom involves separation and purification.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Extraction: Get 1 kg of celery seed and pulverize No. 2 sieves, add diatomite to be 3% (m / m) of the medicinal material quality, add 8 times the amount of 50% ethanol (m / v) to reflux and extract 3 times, each After 1h, the combined filtrate was suction filtered, concentrated under reduced pressure, and concentrated to 1000ml (m / v);

[0051] (2) Purification: Take 1000ml of the extract, add an equal volume of ethyl acetate:n-butanol=2:8 extract, stir for 15min, let stand for half an hour, extract 3 times, collect the organic layer, concentrate under reduced pressure, and dry Obtain the total flavonoid extract;

[0052] (3) Chromatography: get acidic aluminum oxide (pH 2.8~3.8), the weight of described acidic aluminum oxide: the weight of the total flavonoid extract after the purification described in step (1) is 1: 10 (m / m), Pack column, sherwood oil activation; Get the total flavonoid extract after the purification described in step (1), get a small amount of 75% et...

Embodiment 2

[0054] (1) Extraction: Get 1 kg of celery seed and pulverize No. 2 sieves, add diatomite to be 4% (m / m) of the medicinal material quality, add 10 times the amount of 60% ethanol (m / v) to reflux and extract 3 times, each After 1h, the combined filtrate was suction filtered, concentrated under reduced pressure, and concentrated to 1000ml (m / v);

[0055] (2) Purification: Take 1000ml of the extract, add an equal volume of ethyl acetate:n-butanol=1:9 extract, stir for 15min, let stand for half an hour, extract 4 times, collect the organic layer, concentrate under reduced pressure, and dry Obtain the total flavonoid extract;

[0056](3) Chromatography: get acidic aluminum oxide (pH 2.8~3.8), the weight of described acidic aluminum oxide: the weight of the total flavonoid extract after the purification described in step (1) is 1: 15 (m / m), Pack column, sherwood oil activation; Get the total flavonoid extract after the purification described in step (1), get a small amount of 75% et...

Embodiment 3

[0058] (1) Extraction: get 1 kg of celery seed and pulverize No. 2 sieves, add diatomite to be 3% (m / m) of the medicinal material quality, add 12 times of 60% ethanol (m / v) to reflux and extract 2 times, Second 2h, suction filtration combined filtrate, concentrated under reduced pressure, concentrated to 1000ml (m / v);

[0059] (2) Purification: Take 1000ml of the extract, add an equal volume of ethyl acetate:n-butanol=2:8 extract, stir for 15min, let stand for half an hour, extract 4 times, collect the organic layer, concentrate under reduced pressure, and dry Obtain the total flavonoid extract;

[0060] (3) Chromatography: get acidic aluminum oxide (pH 2.8~3.8), the weight of described acidic aluminum oxide: the weight of the total flavonoid extract after the purification described in step (1) is 1: 10 (m / m), Pack column, sherwood oil activation; Get the total flavonoid extract after the purification described in step (1), get a small amount of 75% ethanol and dissolve compl...

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PUM

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Abstract

The invention discloses a celery seed extract and a preparation method thereof. The preparation method comprises the following steps of (1) taking celery seeds, performing crushing, performing screening, adding diatomite which is 3-5% by mass of medicinal materials, adding 50-60% ethanol which is 8-12 times, performing reflow extraction, performing sucking filtration, merging filtrate, and performing decompressed concentration to obtain an extracting solution; (2) taking the extracting solution, adding the extraction solution in which the ratio of ethyl acetate to n-butanol is 2 to 8 or 1 to 9, performing stirring, performing standing, performing extraction, collecting an organic layer, performing decompressed concentration, and performing drying to obtain a total flavonoids extract; and (3) taking acid aluminum oxide, wherein the weight ratio of the acid aluminum oxide to the total flavonoids extract is 1 to (10-20), performing packing, performing activating with petroleum ether, taking the total flavonoids extract, taking the ethanol, performing complete dissolving, adding the acid aluminum oxide, performing stirring, performing evaporating to dryness so as to remove the ethanol,performing sample feeding, performing eluting with the petroleum ether, performing eluting with the ethanol, collecting ethanol effluent, performing drying, and performing detection to obtain a celery seed extract. The content of total flavonoids of celery seeds can be effectively increased, so that the content of the total flavonoids of the celery seeds achieves 80% or above.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a celery seed extract and a preparation method thereof. Background technique [0002] Current studies have shown that celery seed has various pharmacological activities such as anti-oxidation, anti-tumor, lowering blood fat, lowering blood pressure, and analgesia. Celery seed mainly contains volatile oil components, phthalide derivatives, coumarin components, flavonoids and their glycosides. Among them, flavonoids include coyilidin and its glycosides, quercetin and its glycosides, apigenin and its glycosides, luteolin and its glycosides, etc. [0003] The flavonoids contained in celery seed have many physiological functions and medicinal value. Its function is to reduce the fragility of blood vessels, improve the permeability of blood vessels, reduce blood fat and cholesterol, and be used to prevent and treat hypertension and cerebral hemorrhage in the elderly. It al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61K131/00
CPCA61K36/23A61K2236/333A61K2236/53
Inventor 周凯蔡冬青季亚飞
Owner 杨凌萃健生物工程技术有限公司
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