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Treatment method for crushing microalgae with ultrasonic waves

A treatment method, ultrasonic technology, applied in the direction of microorganism-based methods, treatment of microorganisms with electricity/wave energy, treatment of enzymes with electricity/wave energy, etc., can solve problems such as cumbersome operation, loss of biologically active components in cells, and poor crushing effect , to achieve high crushing efficiency, improve the economic benefits of aquatic animal seedling and breeding, and good stability

Pending Publication Date: 2019-12-20
GUANGXI ACADEMY OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the crushing method for microalgae cells has the problems of cumbersome operation, poor crushing effect, and serious loss of biologically active components in cells.
[0007] So far, there have been no reports about breaking microalgae in the form of aggregated colonies, filaments, and long strips between cells without destroying the cell wall and affecting the biologically active components in the cell

Method used

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  • Treatment method for crushing microalgae with ultrasonic waves

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Effect test

Embodiment 1

[0026] A treatment method for crushing Spirulina platensis by ultrasonic waves, comprising the steps of: centrifuging the culture solution of Spirulina platensis cultured aseptically for 5 days at a speed of 5000 rpm for 10 minutes, collecting the algae cell enrichment solution in the lower layer, and obtaining the cell density 1.0×10 7 1 / mL of concentrated algae liquid, add 300mL PBS buffer solution to each gram of concentrated algae liquid, place the mixed material in an ultrasonic cleaner for 20min, the ultrasonic power is 80W, keep the temperature of the mixed material at 24°C during the process , after treatment, Spirulina platensis with a length of 4-8 μm accounts for 88% of the total amount of Spirulina platensis.

Embodiment 2

[0028] A treatment method for crushing Sclerotina costatum by ultrasonic waves, comprising the following steps: centrifuging the culture solution of Sclerotina costatum after 2 days of aseptic culture at a speed of 5200 rpm for 8 minutes, collecting the algae cell enrichment solution in the lower layer, and obtaining the cell density 2.6×10 7 1 / mL of concentrated algae liquid, add 320mL PBS buffer solution to each gram of concentrated algae liquid, place the mixed material in an ultrasonic cleaner for 28min, the ultrasonic power is 65W, and keep the temperature of the mixed material at 28°C during the process After the treatment, more than 85% of the Skeletalum costatum in the mixed material exists in the form of single cells.

Embodiment 3

[0030] A treatment method for crushing Skeletodera tropicalis by ultrasonic waves, comprising the following steps: centrifuging the culture solution of Chaetoceros mouroii for 7 minutes at a speed of 5400 rpm after 2 days of aseptic culture, collecting the algae cell enrichment solution in the lower layer, and obtaining the cell Density is 3.7×10 7 1 / mL of concentrated algae liquid, add 340mL PBS buffer solution to each gram of concentrated algae liquid, place the mixed material in an ultrasonic cleaner for 32min, the ultrasonic power is 70W, keep the temperature of the mixed material at 30°C during the process After the treatment, more than 90% of Chaetoceros mourei in the mixed material exists in the form of single cells.

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Abstract

The invention discloses a treatment method for crushing microalgae with ultrasonic waves. The method comprises the following steps of mixing a concentrated algae solution enriched with microalgae witha PBS buffered solution to obtain a mixed solution, placing the mixed solution in an ultrasonic generation instrument, and performing treatment for 20-40min, so as to microalgae of which the length is 2-30 [mu]m, wherein the shape of the microalgae is any one of an aggregation group, protonema and rectangular strips. The method disclosed by the invention overcomes the technical defect that a conventional physical mechanical crushing method generates uneven and too big cutting force, so that the integrity of the cell body of the microalgae is damaged. According to the method, the cell wall ofthe microalgae is not destroyed, microalgae cenobium in the shape of the aggregation group, protonema and rectangular strips is subjected to intercellular breaking and separation, so that cell massesin rows, groups or clusters are broken to obtain cell rows or cell groups being shorter in length, even the cell masses are broken into single algae cells, and algae intra-cellular nutrient componentsare effectively reserved. The microalgae breaking solution obtained by the method can meet nutrient requirements of aquatic animals for ingestion cabibre at the seedling raising early stage.

Description

technical field [0001] The invention relates to a treatment method for crushing microalgae by ultrasonic waves. Background technique [0002] Algae is the lowest category in the plant kingdom, because their cells contain chlorophyll or other pigments, can carry out photosynthesis, self-synthesize the required nutrients, live independently and mainly reproduce and grow by cell division, so algae belong to the category of natural In trophic eukaryotes, microalgae are difficult to see with the naked eye due to their small size, and generally need to be observed with a microscope. There are more than 20,000 kinds of microalgae, and they are the main primary producers in the water ecosystem, accounting for 40%, providing the most primary productivity for marine organisms. The special growth environment makes it contain a variety of biologically active substances, such as active proteins, vitamins, minerals, carotenoids, microalgae polysaccharides and polyunsaturated fatty acids....

Claims

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Application Information

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IPC IPC(8): C12N13/00C12R1/89C12R1/01
CPCC12N13/00
Inventor 张彬邓潜朱昔恩熊建华谢达祥赵永贞陈田聪陈晓汉韦嫔媛
Owner GUANGXI ACADEMY OF FISHERY SCI
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