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Array structure capable of accurately modifying biomolecules, and modifying method

A biomolecule and array structure technology, applied in the field of precisely modifying the array structure of biomolecules and its modification, can solve problems such as infeasibility, and achieve the effect of precise and precise modification, precise modification, and simple operation

Active Publication Date: 2019-12-24
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide an array structure and modification method for precisely modifying biomolecules, which are used to solve the problems of the prior art Infeasible disadvantages, and can effectively reduce the complexity of the manufacturing process

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  • Array structure capable of accurately modifying biomolecules, and modifying method
  • Array structure capable of accurately modifying biomolecules, and modifying method
  • Array structure capable of accurately modifying biomolecules, and modifying method

Examples

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Embodiment 1

[0053] Example 1 An array structure for precisely modifying biomolecules

[0054] 1. Preparation method

[0055] Such as figure 1 Shown is the flow chart of the preparation method of embodiment 1, comprises the following steps:

[0056] S1. One side of the separation template 1 is provided with 12 hemispherical grooves 10 ( figure 2 ), the laser micro-nano processing manufacture etches the center of the bottom of the groove, and cuts through to form a hole 11 ( image 3 ), wherein the diameter of the groove 10 is 7 μm, and the diameter of the hole 11 is 2 μm;

[0057] S2. Using a coating process to cover a layer of isolation layer 5 on the outer surface of the magnetic bead 2; wherein the diameter of the magnetic bead 2 is 5 μm, the diameter of the groove 10 is 1.4 times the diameter of the magnetic bead 2, and the isolation layer 5 is an alumina isolation layer; The coating process is as follows: the magnetic beads 2 are immersed in the molten isolation layer, and then t...

Embodiment 2

[0063] Example 2 An array structure for precisely modifying biomolecules

[0064] 1. Preparation method

[0065] Such as figure 1 Shown is the flow chart of the preparation method of embodiment 1, comprises the following steps:

[0066] S1. One side of the separation template 1 is provided with 16 hemispherical grooves 10 uniformly distributed in an array. The center of the bottom of the groove is etched by high-energy electron beam micro-nano processing to form a hole 11, wherein the diameter of the groove 10 is 10 μm, the diameter of the hole 11 is 5 μm;

[0067] S2. Using a coating process to cover a layer of isolation layer 5 on the outer surface of the magnetic bead 2; wherein the diameter of the magnetic bead 2 is 10 μm, the diameter of the groove 10 is 1 times the diameter of the magnetic bead 2, and the isolation layer 5 is a titanium oxide isolation layer; The coating process is as follows: the magnetic beads 2 are immersed in the molten isolation layer, and then t...

Embodiment 3

[0073] Example 3 An array structure for precisely modifying biomolecules

[0074] 1. Preparation method

[0075] Such as figure 1 Shown is the flow chart of the preparation method of embodiment 1, comprises the following steps:

[0076] S1. One side of the separation template 1 is provided with 8 hemispherical grooves 10 ( figure 2 ), the laser micro-nano processing manufacture etches the center of the bottom of the groove, and cuts through to form a hole 11 ( image 3 ), wherein the diameter of the groove 10 is 7 μm, and the diameter of the hole 11 is 2 μm;

[0077] S2. Using a coating process to cover a layer of isolation layer 5 on the outer surface of the magnetic bead 2; wherein the diameter of the magnetic bead 2 is 5 μm, the diameter of the groove 10 is 1.4 times the diameter of the magnetic bead 2, and the isolation layer 5 is an alumina isolation layer; The coating process is as follows: the magnetic beads 2 are immersed in the molten isolation layer, and then th...

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Abstract

The invention discloses an array structure capable of accurately modifying biomolecules, and a modifying method. The array structure comprises a separation template and magnetic beads fixed to the separation template; hemispherical grooves are formed in one side of the separation template, holes are formed in the circle centers of the bottoms of the grooves, and the magnetic beads are arranged inthe grooves; isolation layers are arranged on the surfaces of the magnetic beads; nanoholes are formed in the parts, coinciding with the holes, of the isolation layers, and thus the nanoholes communicate with the holes; and the biomolecules are connected with the magnetic beads through the nanoholes. According to the modifying method provided by the invention, accurate assembling of the single magnetic beads and the biomolecules is efficiently achieved, and the important significance for single-molecule detection is achieved; the biomolecules can be efficiently and accurately modified throughthe array structure; and the array structure is placed into a liquid pool containing a solution of the biomolecules needing to be modified, accurate modifying of the biomolecules is simultaneously conducted on the multiple magnetic beads efficiently and accurately through the characteristics of the array structure, and the method is easy to operate, efficient and accurate.

Description

technical field [0001] The invention relates to the technical field of biological sciences, more specifically, to an array structure for precisely modifying biomolecules and a modification method thereof. Background technique [0002] The study of DNA molecular manipulation is an important content of molecular biology research. However, it is affected by the thermal motion of DNA and the complex fluid motion in the nanopore (Li J, Gershow M, Stein D, et al.DNA molecules and configurations in a solid-state nanopore microscope[J].Nature materials,2003,2 (9):611.), which makes it very difficult to detect the DNA pore current signal. Therefore, in order to realize the possibility of solid-state nanopore sequencing, it is very important to use single-molecule manipulation technology to stretch a single DNA single-strand molecular pore. [0003] Traditional single-molecule manipulation techniques include the use of microneedles, atomic force microscopy, optical tweezers, magnetic...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/42C12N15/10
CPCC12N15/10
Inventor 袁志山刘佑明王成勇
Owner GUANGDONG UNIV OF TECH