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Probe and primer composition and kit for detection of gene mutations in heart-related chronic diseases based on multiplex pcr flying nucleic acid mass spectrometry

A primer composition and kit technology, applied in the field of molecular biology, to ensure specificity, high precision, and improve throughput.

Active Publication Date: 2021-04-09
苏州乾康基因有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no risk prediction product on the market that specifically detects gene mutations associated with chronic diseases

Method used

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  • Probe and primer composition and kit for detection of gene mutations in heart-related chronic diseases based on multiplex pcr flying nucleic acid mass spectrometry
  • Probe and primer composition and kit for detection of gene mutations in heart-related chronic diseases based on multiplex pcr flying nucleic acid mass spectrometry
  • Probe and primer composition and kit for detection of gene mutations in heart-related chronic diseases based on multiplex pcr flying nucleic acid mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Primers and Probes

[0038] A primer and probe composition for diagnosing five heart-related chronic diseases, see Table 1 for details.

[0039] Table 2

[0040]

[0041] Table 2 (continued)

[0042]

[0043]

[0044] A detection kit for diagnosing five heart-related chronic disease conditions, which includes the primer and probe compositions shown in Table 1, and also includes other necessary components for detection: 10uM / uL Taq DNA polymerase, 10×PCR buffer solution, 10uM / uL of 4 kinds of dNTP mixture.

Embodiment 2

[0046]In order to verify that this kit can accurately detect polymorphisms of 30 SNP sites in 24 genes, and that the detection results can achieve the effect of prompting the risk of chronic heart diseases, the reagents involved in the present invention are used for A, B and C. box for testing. In particular, A, B and C are around 35 years old, and A is diagnosed with coronary heart disease. At the same time, in order to confirm that the kit involved in the present invention can accurately detect the polymorphisms of more than 30 SNP sites, the results of some sites were verified by first-generation sequencing.

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Abstract

The invention relates to a primer composition for detecting gene mutations, in particular to a probe, a primer composition and a kit for detecting gene mutations of heart-related chronic diseases based on multiple PCR flight nucleic acid mass spectrometry, and belongs to the technical field of molecular biology. The composition has the advantages of high specificity, close annealing temperature, and no secondary structure, and can realize simultaneous, rapid, high-precision, and high-sensitivity multiplexing of 30 risk sites in 24 genes of 5 heart-related chronic diseases PCR fly nucleic acid mass spectrometry technology gene detection. The probe and primer composition involved in the present invention have similar Tm values, and realize the amplification of 30 SNP sites in the same tube, which greatly improves the throughput of one detection; the primer sequences are compared by NCBI, The specificity of amplification is ensured.

Description

technical field [0001] The invention relates to a primer composition for detecting gene mutations, in particular to a probe, a primer composition and a kit for detecting gene mutations of heart-related chronic diseases based on multiple PCR flight nucleic acid mass spectrometry, and belongs to the technical field of molecular biology. Background technique [0002] Chronic diseases, that is, chronic non-infectious diseases, are a general term for a class of diseases that do not constitute infection and have long-term accumulation of disease-like damage. This type of disease usually does not arouse the alertness of patients. It has a long course, slow development and is difficult to cure. The etiology is complex and lacks definite evidence of infectious biological etiology. Among them, heart-related chronic diseases, such as myocardial infarction, coronary heart disease, heart fibrillation, atrial septal defect, and cardiomyopathy are relatively common and extremely harmful ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6858C12Q1/6883
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2565/627C12Q2547/101C12Q2533/101
Inventor 张惠丹戴敬赵捷赵洪玉
Owner 苏州乾康基因有限公司