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Method for assisting identification of root surface characteristics of wheat at seedling stage, and special primer set thereof

An auxiliary identification and surface area technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of lagging research on genetic analysis of wheat root traits

Active Publication Date: 2019-12-27
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The discovery of genes and their genetic markers controlling root traits is the prerequisite for molecular marker-assisted breeding. Compared with the agronomic traits of aboveground parts, the genetic analysis of wheat root traits is relatively lagging behind.

Method used

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  • Method for assisting identification of root surface characteristics of wheat at seedling stage, and special primer set thereof
  • Method for assisting identification of root surface characteristics of wheat at seedling stage, and special primer set thereof
  • Method for assisting identification of root surface characteristics of wheat at seedling stage, and special primer set thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. The acquisition of the SNP site of the gene related to the seedling root system and its marker AX95025477 and special primers

[0046] Yangmai 16 (China Agricultural Development Group Co., Ltd., variety right number: CNA20030436.4) / Zhongmai 895 (Henan Xinda Agricultural Development Co., Ltd., National Review Mai 2012010) DH population was selected, including a total of 200 families including parents.

[0047] figure 1 Seedling root system diagrams of Zhongmai 895 (right) and Yangmai 16 (left).

[0048] 1. Investigation of root traits

[0049] The root test adopts the hydroponic method, that is, 30 seeds of each variety are selected, treated with 10% for 20-30 minutes, and washed with sterile water for 5-6 times. select H 2 o 2 The treated seeds were plump and uniform in size, placed in a Petri dish covered with filter paper, and germinated in the dark room of an incubator. For each material, seeds with consistent germination were selected for seedling cu...

Embodiment 2

[0080] Example 2, Application of SNP AX95025477 Site in Detection of Wheat Root Traits

[0081] Among the 14 varieties: Zhongmai 895 and Yangmai 16 as the control group, others as the test group: Afu, CA1055, CA1133, Lunxuan 987, Jingdong 8, Qinnong 731, Wanmai 52, Jining 16, Jimai 21. Lumai 15, Luomai 21, Mianyang 26, Ningmai 9, Zhenmai 6.

[0082] 1. Routine detection of root traits at the seedling stage of different wheat varieties to be tested

[0083] The identification of root traits of wheat varieties at the seedling stage was carried out in the greenhouse of the Institute of Crop Science, Chinese Academy of Agricultural Sciences in the winter of 2015. For each wheat variety to be tested, select 30 seeds that are plump and of the same size, and use 10% H 2 o 2 Treat for 20-30 minutes, rinse with sterile water 5-6 times; then place the seeds in a petri dish covered with filter paper, and germinate in a dark room for 18-24 hours in an incubator; then select 25 seeds th...

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Abstract

The present invention discloses a method for assisting identification of root surface characteristics of wheat at a seedling stage, and a special primer set thereof, and provides an application of a substance for detecting genotype of SNP AX95025477 locus on wheat chromosome 7BL in identifying or assisting identification of wheat root characteristics; and the SNP AX95025477 locus is the 36th nucleotide sequence shown in a sequence 4. A wheat seedling stage root characteristic-related locus SNP locus AX95025477 is found. The SNP locus can be used to screen wheat with good related root characteristics and plays an important role in cultivating water and fertilizer high-efficiency type wheat varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisting identification of wheat seedling root surface area traits and a special primer set thereof. Background technique [0002] As one of the three major organs of plants, the root system is the first part of mineral nutrient absorption and water conduction, and plays a role in fixing and supporting plant growth (Guo Haiyu, 2013). The first green revolution improved the grain yield by improving the traits of the aboveground parts of crops (such as dwarf breeding) and sufficient supply of fertilizer and water, but the variation of the underground part was not taken seriously. Efficiency plays an important role (Li Jinhua, 2009; Gewin, 2010; Zhao, 2011; Zhang, 2014). Den Herder et al. (2010) pointed out that continuously increasing the amount of fertilizer and water to increase wheat yield is difficult to guarantee planting income. At present, it is necessary to impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 肖永贵武玉莹杨梦娇何中虎夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI