Haloalkaliphilic microbial flora and application thereof

Abstract

The invention discloses a haloalkaliphilic microbial flora and an application thereof, the haloalkaliphilic microbial flora is used for degrading printing and dyeing wastewater, and the application comprises the following steps: (1) screening and domesticating the haloalkaliphilic microbial flora; (2) activating the haloalkaliphilic microbial flora to obtain a bacterial suspension; (3) degrading the dye wastewater; sucking the bacterial suspension obtained in the step (2), adding the bacterial suspension into a triangular flask filled with an inorganic salt culture medium and dye wastewater, and performing standing and culturing in a dark place at the constant temperature of 25-35 DEG C until the wastewater is decolorized. The microbial flora obtained by screening and domesticating is resistant to high-salt and high-alkali environments, and the cost of diluting salinity before degradation of common microorganisms and manually adjusting pH to be neutral is saved.

Description

technical field

[0001] The invention relates to a microbial flora, in particular to a halophilic and alkalophilic microbial flora capable of degrading azo dyes under high-alkali and high-salt conditions and a method for degrading waste water. Background technique

[0002] Printing and dyeing wastewater is the third most polluted wastewater in terms of industrial wastewater discharge. It has the characteristics of deep color, high content of organic pollutants, refractory biodegradation, high salinity, and high alkalinity. When printing and dyeing factories produce consumer goods, the azo dyes used are not only toxic, but also harmful to the growth of animals and plants in the water, directly or indirectly affecting people's lives. In addition to polluting water, azo dyes can also cause direct harm to humans. Due to the azo bond and aromatic ring in the azo dye, it is very likely to produce about 24 kinds of carcinogenic aromatic amine products after the cleavage reaction, w...

Examples

Embodiment 1）

[0030] (Example 1) Preparation method of halophilic and alkaliphilic microbial flora

[0031] ①Add 100 mL of sterilized inorganic salt culture medium and 1.5-2.5 mL (2 mL in this example) to a 250 mL Erlenmeyer flask with a concentration of 0.1-6 g / L (5 g / L in this example) Dye acid golden yellow G, mix well; then add 5g of activated sludge obtained after the wastewater treatment of printing and dyeing factory into the triangular flask and mix well.

[0032] The content of each component in the inorganic salt culture medium used is: Na 2 SO 4 0.4~0.6g / L, NH 4 Cl 0.2~0.4g / L, CaCl 2 0.08~0.12g / L, KH 2 PO 4 0.15~0.3g / L, KCl 0.4~0.6g / L, NaCl 80~90g / L, MgCl 2 10-15g / L; use NaOH solution to adjust the pH value of the inorganic salt medium to 10-12; the solute of the inorganic salt medium is water.

[0033] The content of each component in the inorganic salt medium used in the present embodiment is: Na 2 SO 4 0.5g / L, NH 4 Cl 0.3g / L, CaCl 2 0.1g / L, KH 2 PO 4 0.2g / L...

Embodiment 2）

[0040] (Example 2) Preparation method of halophilic and alkaliphilic microbial flora

[0041] The rest of the preparation method of the halophilic and alkalophilic microbial flora of the present embodiment is the same as in Example 1, except that: Step 1. Add 100 mL of sterilized inorganic salt medium in a 250 mL Erlenmeyer flask and a concentration of 5 g in 2 mL / L of acidic golden yellow G, mix well; then add 10mL of printing and dyeing factory wastewater into the triangular flask and mix well.

Embodiment 3）

[0042] (Example 3) Application of halophilic and alkaliphilic microbial flora

[0043] In this example, the halophilic and alkaliphilic microbial flora was used for the degradation of dye wastewater.

[0044] The degradation method of the dye wastewater of the present embodiment comprises the following steps:

[0045] ① Screening and domestication of halophilic and alkalophilic microbial flora.

[0046] Add 100 mL of sterilized inorganic salt culture medium and 2 mL of labeling dye acid golden yellow G with a concentration of 5 g / L in a 250 mL conical flask, and mix well; then add 5 g of activated sludge obtained from wastewater treatment in printing and dyeing factories and mix well.

[0047] The content of each component in the inorganic salt culture medium used is: Na 2 SO 4 0.5g / L, NH 4 Cl 0.3 g / L, CaCl 2 0.1g / L, KH 2 PO 4 0.2g / L, KCl 0.5g / L, NaCl 85.8g / L, MgCl 2 12.6g / L; adjust the pH value of the inorganic salt medium to 10 with NaOH solution; the solute of t...

technical field

[0001] The invention relates to a microbial flora, in particular to a halophilic and alkalophilic microbial flora capable of degrading azo dyes under high-alkali and high-salt conditions and a method for degrading waste water. Background technique

[0002] Printing and dyeing wastewater is the third most polluted wastewater in terms of industrial wastewater discharge. It has the characteristics of deep color, high content of organic pollutants, refractory biodegradation, high salinity, and high alkalinity. When printing and dyeing factories produce consumer goods, the azo dyes used are not only toxic, but also harmful to the growth of animals and plants in the water, directly or indirectly affecting people's lives. In addition to polluting water, azo dyes can also cause direct harm to humans. Due to the azo bond and aromatic ring in the azo dye, it is very likely to produce about 24 kinds of carcinogenic aromatic amine products after the cleavage reaction, w...

Examples

Embodiment 1）

[0030] (Example 1) Preparation method of halophilic and alkaliphilic microbial flora

[0031] ①Add 100 mL of sterilized inorganic salt culture medium and 1.5-2.5 mL (2 mL in this example) to a 250 mL Erlenmeyer flask with a concentration of 0.1-6 g / L (5 g / L in this example) Dye acid golden yellow G, mix well; then add 5g of activated sludge obtained after the wastewater treatment of printing and dyeing factory into the triangular flask and mix well.

[0032] The content of each component in the inorganic salt culture medium used is: Na 2 SO 4 0.4~0.6g / L, NH 4 Cl 0.2~0.4g / L, CaCl 2 0.08~0.12g / L, KH 2 PO 4 0.15~0.3g / L, KCl 0.4~0.6g / L, NaCl 80~90g / L, MgCl 2 10-15g / L; use NaOH solution to adjust the pH value of the inorganic salt medium to 10-12; the solute of the inorganic salt medium is water.

[0033] The content of each component in the inorganic salt medium used in the present embodiment is: Na 2 SO 4 0.5g / L, NH 4 Cl 0.3g / L, CaCl 2 0.1g / L, KH 2 PO 4 0.2g / L...

Embodiment 2）

[0040] (Example 2) Preparation method of halophilic and alkaliphilic microbial flora

[0041] The rest of the preparation method of the halophilic and alkalophilic microbial flora of the present embodiment is the same as in Example 1, except that: Step 1. Add 100 mL of sterilized inorganic salt medium in a 250 mL Erlenmeyer flask and a concentration of 5 g in 2 mL / L of acidic golden yellow G, mix well; then add 10mL of printing and dyeing factory wastewater into the triangular flask and mix well.

Embodiment 3）

[0042] (Example 3) Application of halophilic and alkaliphilic microbial flora

[0043] In this example, the halophilic and alkaliphilic microbial flora was used for the degradation of dye wastewater.

[0044] The degradation method of the dye wastewater of the present embodiment comprises the following steps:

[0045] ① Screening and domestication of halophilic and alkalophilic microbial flora.

[0046] Add 100 mL of sterilized inorganic salt culture medium and 2 mL of labeling dye acid golden yellow G with a concentration of 5 g / L in a 250 mL conical flask, and mix well; then add 5 g of activated sludge obtained from wastewater treatment in printing and dyeing factories and mix well.

[0047] The content of each component in the inorganic salt culture medium used is: Na 2 SO 4 0.5g / L, NH 4 Cl 0.3 g / L, CaCl 2 0.1g / L, KH 2 PO 4 0.2g / L, KCl 0.5g / L, NaCl 85.8g / L, MgCl 2 12.6g / L; adjust the pH value of the inorganic salt medium to 10 with NaOH solution; the solute of t...