40 results about "Mycobiota" patented technology

The invention discloses a microorganism concrete brick and a preparation method thereof. The microorganism concrete brick is formed by mixing cement, gravel, sawdust (or straw scraps, scraps of paper, and the like), mixing mixed microorganism with water according to a certain proportion, forming by a mold and then naturally hardening, wherein the mixed microorganism comprises six floras including bacillus, nitrobacteria, photosynthetic bacteria, saccharomycetes, lactobacillus and actinomycetes. The microorganism concrete brick has excellent water quality purifying function, can continuously play roles for a long time, and is widely applicable to purify various water qualities such as rivers, lakes, canal water, swimming pools, fishponds and the like.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention discloses an agricultural microbial preparation. The agricultural microbial preparation comprises a mould preparation or / and a bacterial preparation, wherein the mould preparation is prepared by inoculating a specially-prepared liquid culture medium with mould spores to obtain a mould fermentation solution, then preparing a mould inoculum preparing culture medium from soybean meal, corn meal, bran and the like, mixing the two in proportion, performing culturing fermentation, airing and crushing; the bacterial preparation is also prepared by firstly preparing a liquid culture medium, inoculating with bacteria to obtain a bacterial fermentation solution, mixing the bacterial fermentation solution with a bacterial inoculum preparing culture medium in proportion, performing culturing fermentation, airing and crushing. The microbial preparation provided by the invention is environment-friendly and safe, and after application to soil, the microbial preparation plays an effective role in controlling the melon root-knot nematode disease and the watermelon wilt disease, enhances the growth and reproduction of a beneficial bacterial flora of a crop root system, inhibits the growth of a harmful bacterial flora and reduces the occurrence of diseases and pests; pesticides are not applied any more, so that the problem of pesticide residues is avoided, and requirements on production of environment-friendly and healthy agricultural products are met; in addition, crops can grow vigorously, so that the yield and the income are increased.

The invention disclosed a preparing process of microbial population which can be used to treat dying waste liquids. Collect the engineering primitive microbial population from the sewage or silt on the discharging point of the dying factory. Enrich the microbe by inoculating the primitive microbial population on the medium for 5-7d to get the engineering microbial population. Add 5%, 10%, 15%, 20% and 25% dying waste liquids to the microbe medium and habituated cultivate the microbe according to the microbe's cultivating method for 7-10d to get habituated microbial population. The invention has many advantages such as economical, efficient, safe, great adaptivity and no secondary pollution, so it's an ideal microbial population to treat dying waste liquids.

The invention belongs to the technical field of organic agricultural product production, and particularly provides a method for planting organic strawberry by a micro-ecological technology and a preparation of a used micro-ecological bacteria liquid. In particular, the micro-ecological bacteria liquid is obtained by preparing a plurality of stock solutions. The components of the stock solutions comprise a photosynthetic bacterium stock solution, a beauveria bassiana stock solution, trichoderma culture, pathogenic fungus protein and a biological organic bacterial fertilizer (nutritive water). The bacteria liquid can be used as a medicament for sterilizing and killing worms and also can be used as an organic fertilizer for using. The high-quality organic strawberry which meets the requirement of OFDC (Organic Food Development Center) standard can be produced by only spraying the bacterial liquid regularly. By adjusting and controlling a micro-ecological environment of microorganism, an effective microbial community in a microbial community in a plant body after spraying the micro-ecological bacteria liquid can be changed into a dominant bacterial community, so that effects such as nutrition competition of the dominant bacterial community and the like can be fully played, the number of harmful bacterial communities is controlled below an economic harm level, and a maladjusted microecosystem can be restored into balance, so that the best economic, ecological and social benefits are obtained.

The invention discloses a quick sludge composting method for co-drying of earthworm compost. The method comprises the following steps that (1) after sludge earthworm casts is laid on a middle-layer earthworm bed, earthworms are inoculated, and after sludge is granulated, a top-layer earthworm ridge is carpeted with sludge particles; (2) a lower-layer earthworm collection bed, the middle-layer earthworm bed and the top-layer earthworm ridge are stacked, the ridge is covered with breathable black-out cloth, and the earthworms crawl from the middle layer to the upper layer to treat the sludge particles for 7-10 days; (3) the top-layer earthworm ridge is naturally air-dried for 2 days and taken away, and the earthworms automatically crawl into the middle-layer earthworm bed; (4) fresh earthworm casts in the middle-layer earthworm bed are shoveled out to prepare a turbid solution for inoculating florae, the turbid solution is sprayed in the sludge in the top-layer earthworm ridge, and stabilizing is carried out for 7-10 days to form the earthworm compost; (5) a new earthworm ridge is added on the middle-layer earthworm bed as the top-layer earthworm ridge, the operation processes are repeated, and the earthworm compost can be obtained constantly. The quick sludge composting method for co-drying of the earthworm compost has the advantages that the sludge composting treatment cycle ofthe earthworms is shortened, continuous treatment of the sludge earthworm casts can be achieved, the earthworms do not need to be taken out or sorted, and the water content of the earthworm compost is low.

The re-ferment deodorization of urban garbage is the adding of mixed deodorization bacteria into the cleaned garbage, namely Bullera sinensis Li CGMCC 2.1614, Rhodotorula minuta CGMCC 2.277, Candida tropicalis CGMCC 2.1387 and myces kurssanovii CGMCC 4.509. It can use all kinds of bacteria to remove odors released from fermented biology.

The invention belongs to the technical field of organic agricultural product production, and particularly provides a method for cultivating organic muskmelons by using a microecology technology and preparation of adopted micro-ecologic bacteria liquid. Specifically, the micro-ecologic bacteria liquid is obtained by preparation of a plurality of undiluted solutions; the undiluted solutions contain ingredients of heterologous fungus protein, beauveria bassiana undiluted solution, photosynthetic bacteria undiluted solution and biological organic bacterial manure (nutritive water). The bacteria liquid provided by the invention can be used both as a fungicidal and pesticidal chemical and organic fertilizer; and organic muskmelon of high quality, which meets the OFDC (Organic Food Development Center) standard requirements, can be produced only by spraying the bacteria liquid at regular intervals. By regulating and controlling the micro-ecologic environment of microorganism, after being sprayed with the micro-ecologic bacteria liquid, the beneficial flora of the microorganism flora in the plant becomes the dominant flora, so that the beneficial flora can give full play of nutrition competition and the like so as to control the number of the harmful flora under an economic injury level and rebalance the disordered micro-ecologic system, thereby obtaining the best benefits on economy, ecology and society.

The invention discloses a bacterial preparation for killing wireworm and a preparation method thereof. The bacterial preparation is a compound colony formed by the optimized combination of bacillusmegaterium, pseudomonas flu orescens and staestomyces microflavus, wherein the rate of the number of colonies of all the bacteria is 1:0.5-1:1-1.5, and absorptive carrier is humic acid. The preparation method of the bacterial preparation includes three-stage liquid enlarging culture and the liquid is absorbed on the carrier. Once applied to soil, effective bacteria can quickly form advantageous colonies, brings the function of killing wireworm into play with the killing rate being 94-98%. The mutual match of three bacteria not only kills and expels worms, but also promotes and restores the ecological environment of soil.

The invention relates to the technical field of fertilizers, and in particular relates to a novel environment-friendly bio-organic fertilizer for black leather sugarcane. The environment-friendly bio-organic fertilizer comprises an organic matter, alcohol waste liquid, beneficial biological flora and micro-elements, wherein the organic fertilizer comprises the following components in parts by weight: 15-25 parts of organic matter, 70-80 parts of alcohol waste liquid and 1-2 parts of micro-elements; the content of probiotics in the organic fertilizer is 300000000-400000000 probiotics / g. The novel environment-friendly bio-organic fertilizer disclosed by the invention can be used for achieving the resource utilization for producing wastes, is economic and environment-friendly and complete in nutrient, has the effects of promoting roots, enhancing seedlings and activating soil, and can be used for rapidly and durably forming the population advantages of beneficial microorganisms, inhibiting the growth and reproduction of harmful pathogenic bacteria, fundamentally changing the micro-ecological environment of the soil, preventing and treating the occurrence of lotus root rot diseases, forming a natural barrier for blocking the harmful pathogenic bacteria, promoting the production increase of black leather sugarcane and increasing the sugar content of black leather sugarcane.

The invention discloses a mixed microorganism flora for promoting growth of plant roots, and belongs to the technical field of agricultural microorganisms. The mixed flora is composed of bacillus megatherium, pseudomonas brassicacearum and azospirillum brasilense; the mixed microorganism flora is prepared by adopting a following method. The preparation method comprises the steps that the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are separately inoculated in an LB culture medium, vibration culture is conducted at 30 DEG C until the bacterial concentration O.D600 is about 2.0, then the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are mixed according to the volume ratio of 1:1:1, and the mixed microorganism flora is obtained. The mixed microorganism flora is superior to a single bacterial strain, all the bacterial strains have a synergistic effect, a significant effect of promoting growth of plant root systems is achieved, and the mixed microorganism flora quite highly adapts to actual soil rhizosphere complex environments.

The invention relates to early flowering Chia (Salvia hispanica L.) strains that are suitable for culturing in a temperate area for seed production. Mutations are introduced to wild type chia seeds. Desired mutant progeny having normal appearance and an altered flora organ development is subsequently identified.

A biological demanganization function strain is disclosed, in particular to a microbe. The invention solves the establishment of the prior biological oxidation demanganization function strain which depends on natural selection, weak biochemical capability and bad demanganization effect and long cultured, tamed, and starting time of the water disposal demanganization biological filter tank establishment and long restoration period after the function strain is broken. The biological demanganization function strain MB4 (Exiguobacterium sp.) belongs to the microminiature Bacterium and is preserved in the Chinese microbe bacterial preservation management committee common microbe center and the preservation date is Oct. 30, 2007 and the preservation number is CGMCC No.2237. The bacterial MB4 is Gram-positive microaerobic bacteria which is bacillus brevis and small in length and is 1-1.5 mum in length, 0.5-0.8 mum in breadth. Two ends of the cell are blunt circle and form brown colony on the culture medium without transparent and the central color is deeper and the peripheral color is lighter. The entire colony is round shape. The strain MB4 has power storage particle without spore and capsule. The dyeing of N, N, N', N'-tetramethyl p-phenylene diamine is positive. The biological demanganization function strain MB4 of the invention is provided with Mn 2+ removing ability and the Mn 2+ clearance is over 91%.

The invention relates to a biological demanganization and deferrization functional bacterium, which relates to a microorganism. The invention solves the problems that: the prior biological oxidation demanganization engineering flora is established in virtue of natural selection, thereby the biochemical capacity is weak and the demanganization effect is bad; the culture, acclimation and starting time required for a water treatment demanganization biological filter chamber is long, and the restore cycle after the enginerring flora is damaged is long. The biological demanganization and deferrization functional bacterium MS604 (Sphingobacterium sp.) belongs to Sphingobacterium sp. and is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation date is October 30th, 2007 and the preservation No. is CGMCC No.2240; the strain MS604 is a gram negative aerobic bacterium and a polymorphous bacillus with a length of 1 to 2 micrometers and a width of 0.3 to 0.4 micrometers, and both ends of cells are round and obtuse; a milk yellow colony is formed on a culture medium and is round and opaque with tidy edges; the strain MS604 is provided with energy storage particles and also provided with a brood cell and a capsule, and N, N, N', N'-tetramethyl-p-phenylenediamine is dyed to be masculine. The biological demanganization and deferrization functional bacterium MS604 has the capacity for removal of Mn<2+> and Fe<2+> in water; the removal rate of Mn<2+> is higher than 79 percent and the removal rate of Fe<2+> is higher than 90 percent.

The invention discloses a microbial fertilizer, which comprises, by weight, 1 to 3 parts of rhizobium, 1 to 3 parts of photosynthetic bacteria, 1 to 3 parts of azotobacter, 1 to 3 parts of potassium and phosphate decomposition bacteria, 3 to 5 parts of soil fungicide, 20 to 30 parts of plant ash, 5 to 10 parts of straw, 5 to 10 parts of pig and cow manure, 3 to 5 parts of sawdust, 3 to 5 parts ofrice bran and several parts of rot leafy soil, medium and water. In preservation or use of the microbial fertilizer, the active environment of bacteria can be improved, nutrients for can be provided for microorganisms in the bacterial fertilizer, the formation of bacteria can be promoted, and the activity of bacteria and the application effect of the microbial fertilizer can be improved.

The invention relates to the field of fertilizer, in particular to lychee tree planting fertilizer. The lychee tree planting fertilizer comprises, by weight, 2-6 parts of mycorrhiza fungi, 2-4 parts of streptomyces jingyangensis, 1-2 parts of bacillus subtilis, 3-5 parts of azotobacter vinelandii, 0.5-1 part of saccharomycetes, 1-4 parts of phosphobacteria, 35-45 parts of organic fertilizer, 35-45 parts of inorganic fertilizer, 15-20 parts of rice chaff, 8-14 parts of humic acid, 3-7 parts of urea acetaldehyde and 3-9 parts of iso-butylidene diurea. The lychee tree planting fertilizer is stable in performance, wide in function, free from toxic and side effect and good in application prospect; various flora promote and complement mutually, crop output is increased, taste is improved, and succulent and refreshing taste is achieved.

The invention provides a preparation method of a deadwood and leaf organic fertilizer capable of growing lichens. The preparation method comprises the following steps: (1) carrying out insolation and sorting; (2) screening and crushing; (3) mixing and inoculating; (4) carrying out matured composting; (5) carrying out separated culture of lichenous algae and symbiotic bacteria; (6) culturing mycobiont; (7) mixing and stirring; (8) prilling; and (9) packaging products. By taking deadwoods and loess swept in streets as primary raw materials, an organic fertilizer containing beneficial bacteria as dominant bacterial communities is prepared by fermenting and composting various beneficial microorganisms and then inoculating mycobiont. By applying the fertilizer, the growth of plant diseases and insect pests and weeds is inhibited by means of bioactivity generated by plant roots, and lichens also grow for eating for people and animals.

The invention discloses pest-expelling protein peptide fertilizer. The pest-expelling protein peptide fertilizer is prepared from, by weight, 30-40 parts of withered leaves, 1-2 parts of rhizopus oryzae, 6-8 parts of ammonium chloride, 55-60 parts of pig manure, 4-5 parts of garlic, 2-3 parts of bacillus subtilis, 3-4 parts of ammonium sulfate, 10-12 parts of castor-oil plant stem leaf decoction, 80-90 parts of almond residues, 2-3 parts of ferrous chloride, 1-2 parts of glutamic acid, 5-7 parts of sodium silicate, 1-2 parts of calcium chloride and an appropriate amount of water. The fertilizer has a good pest expelling effect, the added bacillus subtilis and rhizopus oryzae can promote formation of superior florae of soil and restrain growth of pathogenic bacteria, the added castor-oil plant stem leaf decoction and garlic fermented material can effectively expel pests, and the fertilizer is high in fertilizer efficiency, does not damage soil, can achieve harmless crop planting after being used, increases the crop yield and improves the crop quality.

The invention relates to a method for establishing a ramie phloem fiber biological degumming microecosystem, which comprises the following steps: bacterial sources are collected from a plurality of rotten ramie habitats; and ramie phloem is taken as a single carbon source and a single energy, subjected to enrichment culture for a short period and mixed with the bacterial sources in equal proportion; the ramie phloem is taken as the single carbon source and the single energy and subjected to directional acclimatization at a temperature of between 40 and 43 DEG C and at an initial pH value of 7.5; the ramie phloem is subjected to refueling and repeated inoculation when being separated into the shape of cotton, wherein the inoculum size is 10 percent of the total volume of a culture solution, and the accumulated acclimatization time is between 1 and 2 years; the degumming microecosystem is established after gradual removal of mixed bacteria; floras are subjected to cross-feeding in the system, and secrete extracellular enzymes to highly efficiently degrade the colloids; and the flora structure and the functional stability in the system are maintained by means of transfer of living bodies. The method has the advantages of quick speed of acquisition of high-efficiency degumming strains, extensive varieties of degumming enzymes, high appetency of the enzymes and ramie colloid substrates, strong mixed bacteria resistance of the floras, difficulty of causing degeneration of the degumming capacity, etc.

The invention relates to a fertilizer field, and particularly relates to a pear planting fertilizer. The pear planting fertilizer is prepared from, by weight, 0.5-1.5 parts of polyacrylamide, 2-6 parts of mycorrhiza fungi, 2-4 parts of streptomyces jingyangensis, 1-2 parts of bacillus subtilis, 3-5 parts of brown nitrogen-fixing bacteria, 0.5-1 part of aspergillus oryzae, 1-4 parts of phosphobacteria, 35-45 parts of organic fertilizer, 35-45 parts of inorganic fertilizer, 15-20 parts of rice chaff, 8-14 parts of citric acid, 3-7 parts of butene diurea, 3-9 parts of isobutylidene-diurea. The fertilizer is stable in performance, wide in function, free from toxic and side effects, and has good application prospect. Many kinds of flora can promote and supplement with each other, accelerate the degrading of organic matters, provide rich nutrition, improve crop output, and make taste more cool and juicy.

The invention relates to the technical field of pig fodder, in particular to a method for fermenting pig fodder through microbial flora. According to the method, raw materials such as bean pulp, ricebran, sweet potato residues, distillers' grains, corn flour and Chinese herbal medicine powder are fermented through the microbial flora for making the pig fodder, through the fermentation, the conversion efficiency of the fodder is increased, the quality of the fodder is improved, the taste of the fodder is improved, so that the fodder becomes crisp and soft, and the palatability is improved; meanwhile, the content of antinutritional factors and harmful microorganisms in the raw materials is lowered, and the breeding cost is lowered. The made pig fodder is good in palatability and convenientto digest, feeding of the fine fodder is reduced, the cost is lowered, weight increase of live pigs is effectively promoted, and the pork quality is improved, so that the pork is fresh, tender and delicious. After the pigs eat the fodder, the immunity of the pigs is improved, diseases are reduced, the appetite can be improved, the intake amount is increased, and the pork quality is improved.

The invention relates to organic orchid nutrient soil which comprises in parts by weight, 8-11 parts of sodium heteroauxin, 5-8 parts of rootone, 8-10 parts of monopotassium phosphate, 3-6 parts of turf, 4-8 parts of radiation pine barks, 3-7 parts of perlite, 8-10 parts of BFA (biological fulvic acid), 7-9 parts of bifid bacteria, 3-5 parts of saccharomycetes and 20-30 parts of water. The organic orchid nutrient soil is rich in profitable strains of orchid, complete nutrient components of comprehensive nutrient elements, simple in preparation and high in practicability, and solves the problem of unbalanced supply of nutrient in common nutrient solution, contained rich microelements have remarkable antibacterial, antiviral and parasite expelling effects, the elements in an activated state are quite easily absorbed by plants after special treatment, and the organic orchid nutrient soil has great practical popularization values.

The invention discloses a haloalkaliphilic microbial flora and an application thereof, the haloalkaliphilic microbial flora is used for degrading printing and dyeing wastewater, and the application comprises the following steps: (1) screening and domesticating the haloalkaliphilic microbial flora; (2) activating the haloalkaliphilic microbial flora to obtain a bacterial suspension; (3) degrading the dye wastewater; sucking the bacterial suspension obtained in the step (2), adding the bacterial suspension into a triangular flask filled with an inorganic salt culture medium and dye wastewater, and performing standing and culturing in a dark place at the constant temperature of 25-35 DEG C until the wastewater is decolorized. The microbial flora obtained by screening and domesticating is resistant to high-salt and high-alkali environments, and the cost of diluting salinity before degradation of common microorganisms and manually adjusting pH to be neutral is saved.

The invention provides a Fen-flavor baijiu brewing method based on a jungle fermentation technology. According to the technical scheme, a conventional solid koji cake is improved into a porous structure in the koji making process, so that flora advantage establishment of internal aerobic microorganisms is facilitated promoted. The whole fermentation process is in a jungle environment, and the growth and metabolism of microorganisms can be promoted based on good ecological environments and proper temperature and humidity conditions in jungles, so that the overall fermentation quality is improved. On the basis, supernatant is taken out regularly in the primary fermentation process, and aldehyde substances are prevented from being generated by excessive fermentation of the supernatant as a substrate. The extracted liquid is refrigerated after low-temperature sterilization and is mixed during secondary fermentation, so that the possibility of infectious microbe pollution is reduced. Fen-flavor baijiu prepared through the method is higher in total ester content, and peculiar smell components such as ethyl hexanoate and ethyl butyrate are remarkably reduced; meanwhile, almost no components such as aldehydes, proteins and solid matter remain, so that the mouth feel is purer and lasting, and excellent quality is achieved.

The invention discloses a formula of special nutrient soil for upscale orchid in Jinpeng orchidaceae. According to the formula of the special nutrient soil for the upscale orchid in Jinpeng orchidaceae, the special nutrient soil is prepared from various materials of imported grass charcoal, granular pine bark, hickory nut shell, a mixed organic fertilizer, a beneficial bacterial fertilizer, mica ore powder, granular bamboo charcoal and bone meal. The formula of the special nutrient soil for the upscale orchid in Jinpeng orchidaceae has the advantages that the special nutrient soil is rich in orchid beneficial bacteria and comprehensive nutrient elements, the nutritional ingredients are complete, the preparation is simple, the practicability is high, the problem of unbalanced nutrient supply in a general nutrient solution is solved, the contained abundant trace elements have significant antibacterial, antiviral and antiscolic effects; various elements are in an active state of being absorbed by plants extremely easily after specially treated , and has great promotion and practical value.

The invention discloses a tree foliar fertilizer. The tree foliar fertilizer is prepared from the following formula components in parts by mass: 1-3 parts of urea-nitrogen, 2-4 parts of an insecticide, 1-2 parts of a microorganism population, 3-5 parts of water-soluble potassium, 8-10 parts of Chinese medicinal herbs, 10-20 parts of fine sandy soil, 4-6 parts of a potassium fertilizer, 2-4 parts of natural vitamin B2 and 1-2 parts of a plant growth regulator. The tree foliar fertilizer disclosed by the invention can provide nutrients needed by trees, has a good absorption capability and is not easy to volatilize and disperse.

The invention belongs to the technical field of microorganisms, and relates to a psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and a preparation method of the psychrophilic microbial flora. The preparation method of the psychrophilic microbial flora activated by a cold waterlogged paddy field comprises the steps of mixing and inoculating more than two psychrophilic microbial strain culture liquid into nutrient nutrient broth, cultivating at constant temperature of 12-15 DEG C until the quantity of the strains achieves more than 1.0*10<10>CFU / mL to obtain the psychrophilic microbial flora; evenly adding 3-8% of psychrophilic microbial flora to an attachment carrier of the psychrophilic microbial flora according to the weight percent; and cultivating at constant temperature of 10-15 DEG C until the quantity of the strains achieves the condition that the quantity of effective living bacteria is more than 1.0*10<9> CFU / mL, so as to obtain the psychrophilic microbial flora activated by cold waterlogged paddy field nutrients. The psychrophilic microbial flora prepared by the method effectively prevents the phenomena of stifle of rice plant and runt seedling of paddy of the cold waterlogged paddy field, and promotes the crop to easily grow and rapidly develop, reduces the dosage of a chemical fertilizer, and benefits for conservation of cost.

A biological demanganization function strain is disclosed, in particular to a microbe. The invention solves the establishment of the prior biological oxidation demanganization function strain which depends on natural selection, weak biochemical capability and bad demanganization effect and long cultured, tamed, and starting time of the water disposal demanganization biological filter tank establishment and long restoration period after the function strain is broken. The biological demanganization function strain MB4 (Exiguobacterium sp.) belongs to the microminiature Bacterium and is preserved in the Chinese microbe bacterial preservation management committee common microbe center and the preservation date is Oct. 30, 2007 and the preservation number is CGMCC No.2237. The bacterial MB4 is Gram-positive microaerobic bacteria which is bacillus brevis and small in length and is 1-1.5 mum in length, 0.5-0.8 mum in breadth. Two ends of the cell are blunt circle and form brown colony on the culture medium without transparent and the central color is deeper and the peripheral color is lighter. The entire colony is round shape. The strain MB4 has power storage particle without spore and capsule. The dyeing of N, N, N', N'-tetramethyl p-phenylene diamine is positive. The biological demanganization function strain MB4 of the invention is provided with Mn 2+ removing ability and the Mn 2+ clearance is over 91%.