Bacterial preparation for killing wireworm and preparation method thereof

A technology of bacterial preparations and nematodes, which is applied in the field of fungal preparations and preparations for killing soil nematodes, and can solve the problems of no high density

Inactive Publication Date: 2009-10-28
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There have been some studies at home and abroad, but there have been no reports of

Method used

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  • Bacterial preparation for killing wireworm and preparation method thereof

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Embodiment 1

[0021] Follow these steps:

[0022] 1) The slant strains of Bacillus megaterium, Pseudomonas fluorescens and Streptomyces microflavus preserved at 4°C were activated and cultured in shake flasks respectively. Cultured in soluble starch medium, the specific formula is as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, distilled water 1000mL, pH7.2, autoclave at 121℃ for 30min. Incubate at 28°C for 24h.

[0023] 2) Secondary liquid expansion culture (6L), replace the soluble starch in the above medium with cornmeal, add 0.1% humic acid (mass fraction), inoculum size 5%, culture at 28°C for 24h. .

[0024] 3) Tertiary liquid expansion culture (60 L), the medium is the same as above; the inoculum size is 10%, and cultured at 28° C. for 24 hours.

[0025] 4) Using humic acid as a carrier to fix and adsorb the bacterial solution on the humic acid.

Embodiment 2

[0027] Follow these steps:

[0028] 1) Three strains of Bacillus megaterium, Pseudomonas fluorescens and Streptomyces microflavus preserved at 4°C were activated on slant surfaces, and cultured in shake flasks respectively. Expand the culture with soluble starch medium, the specific formula is as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, distilled water 1000mL, pH7.0, autoclave at 121℃ for 30min. Incubate at 28°C for 12h.

[0029] 2) Secondary liquid expansion culture (10L), replace the soluble starch in the above medium with cornmeal, add 0.1% humic acid (mass fraction), inoculum size 5%, culture at 28°C for 12h. .

[0030] 3) Tertiary liquid expansion culture (100 L), the medium is the same as above, the inoculum size is 10%, and cultured at 28° C. for 12 hours.

[0031] 4) Using humic acid as a carrier to fix and adsorb the bacterial solution on the humic acid.

Embodiment 3

[0033] Follow these steps:

[0034] 1) Three strains of Bacillus megaterium, Pseudomonas fluorescens and Streptomyces microflavus preserved at 4°C were activated on slant surfaces, and cultured in shake flasks respectively. Expand the culture with soluble starch medium, the specific formula is as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.01g, distilled water 1000mL, pH7.0, autoclave at 121℃ for 30min. Incubate at 28°C for 12h.

[0035] 2) Secondary liquid expansion culture (20L), replace the soluble starch in the above medium with cornmeal, add 0.1% humic acid (weight fraction), inoculum size 5%, culture at 28°C for 24h. .

[0036] 3) Tertiary liquid expansion culture (200 L), the medium is the same as above, the inoculum size is 10%, and cultured at 28° C. for 24 hours.

[0037] 4) Using humic acid as a carrier to fix and adsorb the bacterial solution on the humic acid, the weight ratio of the humic acid to the ...

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Abstract

The invention discloses a bacterial preparation for killing wireworm and a preparation method thereof. The bacterial preparation is a compound colony formed by the optimized combination of bacillusmegaterium, pseudomonas flu orescens and staestomyces microflavus, wherein the rate of the number of colonies of all the bacteria is 1:0.5-1:1-1.5, and absorptive carrier is humic acid. The preparation method of the bacterial preparation includes three-stage liquid enlarging culture and the liquid is absorbed on the carrier. Once applied to soil, effective bacteria can quickly form advantageous colonies, brings the function of killing wireworm into play with the killing rate being 94-98%. The mutual match of three bacteria not only kills and expels worms, but also promotes and restores the ecological environment of soil.

Description

technical field [0001] The invention relates to the technical field of environmental biology, in particular to a preparation for killing soil nematodes and a preparation method thereof. Background technique [0002] The significant increase in soil nematodes and the increasing variety and scope of plant diseases are evidence of the destruction of soil ecosystems. Among them, soil nematodes are soil pests that directly affect the yield of crops, especially in the soil where peanuts and sweet potatoes are planted. Nematodes can eat up or destroy peanuts and sweet potatoes in a whole piece of land, and the harvest is almost impossible. In the past, only chemical pesticides were sought, such as Aldicarb, which was effective in the first year and almost ineffective in the second year, and the soil pollution it caused could not be eliminated for decades. Therefore, research and development of microbial agents to kill nematodes is imperative. There have been some studies at home ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P5/00A01P21/00C12N1/20C12R1/11C12R1/39C12R1/01
Inventor 张清敏齐建超薛洪涛崔合香崔小会
Owner NANKAI UNIV
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