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Agricultural microbial preparation and application thereof to control of melon root-knot nematode disease and watermelon wilt disease

A microbial preparation and preparation technology, applied in the field of microbial preparations, can solve problems such as human health hazards, increased energy consumption, soil environmental pollution, etc., and achieve the effects of reducing the occurrence of diseases and insect pests, enhancing stress resistance, and restraining growth

Active Publication Date: 2017-10-27
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in the prevention and control of watermelon wilt, melon farmers still generally use pesticides. Although some achievements have been made in the prevention and control of watermelon People's health awareness is constantly improving, and there is more and more demand for organic melons and fruits. The strong efficacy of pesticides will cause some residues on melons and fruits while preventing and treating watermelon wilt, which is harmful to human health. On the other hand, the large-scale use of pesticides will not only produce drug resistance, but also bring serious burdens to the soil, causing soil environmental pollution, reducing soil fertility and increasing energy consumption.

Method used

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  • Agricultural microbial preparation and application thereof to control of melon root-knot nematode disease and watermelon wilt disease
  • Agricultural microbial preparation and application thereof to control of melon root-knot nematode disease and watermelon wilt disease
  • Agricultural microbial preparation and application thereof to control of melon root-knot nematode disease and watermelon wilt disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 200 g of peeled and cut potatoes into 1400 mL of deionized water, boil at 100 ° C for 10 minutes, filter through three layers of gauze and set the volume to 1000 mL as a solvent; weigh 20 g of glucose, add it to the solvent and stir evenly, and pack in In the triangular flask, seal the package and sterilize it in a sterilizing pot at 121°C for 20 minutes. After cooling, it will be the prepared mold liquid culture medium;

[0034] The mold liquid culture is based on the inoculation ring of mold spores—Paecilomyces lilacinus—inoculated on the aseptic operation table, and the sealed package is placed in a shaker at 28°C for 4 days to obtain the mold—Paecilomyces lilacinus fermentation broth. Leave it for 5 days to make the liquid surface form a layer of spores for later use;

[0035] Weigh 100 g of soybean meal, 400 g of cornmeal, 200 g of bran, and 300 g of sawdust in a fermenter and stir evenly, then add 600 mL of deionized water and mix evenly, seal the package and ...

Embodiment 2

[0038] Weigh 5 g of beef extract, 10 g of peptone, and 5 g of sodium chloride into a measuring cup, add deionized water to 1000 mL, stir evenly and adjust the pH to 7.0~7.2, then pack them into Erlenmeyer flasks, seal and store Sterilize in a sterilizing pot at 121°C for 20 minutes, and wait to cool to obtain a bacterial liquid culture medium;

[0039] Inoculate the sterilized and cooled liquid culture medium on the aseptic operating table with bacteria—Bacillus subtilis, seal the package and put it in a shaker at 36°C for 24 hours to obtain the bacteria—Bacillus subtilis fermentation broth;

[0040] Weigh 180 g of soybean meal, 300 g of cornmeal, 320 g of bran, and 200 g of sawdust in a fermenter and stir evenly, then add 600 mL of deionized water and mix evenly, seal the package and sterilize it in a sterilizing pot at 121°C for 40 minutes. Acquired bacterial agent preparation medium;

[0041] Inoculate 400 mL of bacteria—Bacillus subtilis fermentation broth on the aseptic ...

Embodiment 3

[0043] The method in Example 2 was adopted to prepare bacterial liquid culture medium. Bacterial liquid culture is based on the inoculation of bacteria-Pseudomonas fluorescens on the aseptic operation table, and the sealed package is placed in a shaker at 36°C for 24 hours to obtain the bacteria-Pseudomonas fluorescens fermentation broth;

[0044] Adopt the method in the embodiment 2 to prepare bacterial inoculum preparation culture medium. After cooling to room temperature, the preparation and cultivation of the bacterial agent is based on inoculating 400 mL of bacteria—Pseudomonas fluorescens fermentation broth on the aseptic operation table, stirring evenly, sealing the package and placing it in an incubator at 36°C for 24 hours. After successful fermentation Air-dried in a cool place and crushed into powder to obtain bacteria—Pseudomonas fluorescens biological agent T3.

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Abstract

The invention discloses an agricultural microbial preparation. The agricultural microbial preparation comprises a mould preparation or / and a bacterial preparation, wherein the mould preparation is prepared by inoculating a specially-prepared liquid culture medium with mould spores to obtain a mould fermentation solution, then preparing a mould inoculum preparing culture medium from soybean meal, corn meal, bran and the like, mixing the two in proportion, performing culturing fermentation, airing and crushing; the bacterial preparation is also prepared by firstly preparing a liquid culture medium, inoculating with bacteria to obtain a bacterial fermentation solution, mixing the bacterial fermentation solution with a bacterial inoculum preparing culture medium in proportion, performing culturing fermentation, airing and crushing. The microbial preparation provided by the invention is environment-friendly and safe, and after application to soil, the microbial preparation plays an effective role in controlling the melon root-knot nematode disease and the watermelon wilt disease, enhances the growth and reproduction of a beneficial bacterial flora of a crop root system, inhibits the growth of a harmful bacterial flora and reduces the occurrence of diseases and pests; pesticides are not applied any more, so that the problem of pesticide residues is avoided, and requirements on production of environment-friendly and healthy agricultural products are met; in addition, crops can grow vigorously, so that the yield and the income are increased.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to an agricultural microbial preparation, and also relates to the application of the microbial preparation in preventing and controlling melon root-knot nematode and watermelon wilt. Background technique [0002] Root-knot nematode infection of crops, especially for melons, fruits and vegetables cultivated in protected areas and off-season cultivation, is a very common phenomenon of pests and diseases. The color of vegetable leaves becomes pale or yellow, and the root system of the plant is damaged, resulting in a decrease in the ability of the plant to absorb nutrients, resulting in short plants, weak growth, rigid fruit, cessation of growth, and reduced yield. In severe cases, it will lead to crop death and failure. [0003] At present, with the improvement of human living standards and the increasing demand for green food, biopesticides are favored by growers of melons, fruits and veget...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/02A01N63/04A01P5/00A01P3/00C12R1/79C12R1/69C12R1/885C12R1/645C12R1/685C12R1/125C12R1/12C12R1/39
CPCA01N63/10A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 陈继峰张晓雨刘洪源周艺凯钟贤宇孙佳宁侯贝刘宇翔
Owner ZHENGZHOU UNIV
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