Device for fixing whole body tissue by perfusion of animal heart

Abstract

The invention relates to the technical field of experimental animals, and in particular to a device for fixing whole body tissue by perfusion of an animal heart. The device for fixing the whole body tissue by perfusion of the animal heart comprises a pressure pump, a buffer bottle, a washing liquid bottle and a fixing liquid bottle; the pressure pump is connected with the air inlet end of the buffer bottle through an air guide pipe; the air inlet end of the washing liquid bottle and the air inlet end of the fixing liquid bottle are connected with the air outlet end of the buffer bottle separately through corresponding air supply pipes; and the air outlet end of the washing liquid bottle and the air outlet end of the fixing liquid bottle are connected with liquid outlet pipes. By using thedevice, animal whole body tissue cells can be quickly and efficiently fixed synchronously through animal anesthesia and animal thoracotomy; and meanwhile, more complete tissue proteins and nucleic acid molecules can be obtained simultaneously to provide high-quality experimental specimens for later experiments.

Description

technical field [0001] The invention relates to the technical field of experimental animals, in particular to a device for animal heart perfusion and fixation of whole body tissues. Background technique [0002] Animal tissue fixation is the first step in life science research, which provides an experimental basis for qualitative and quantitative analysis of proteins, detection of nucleic acid molecules, and morphological observation of the fine structure of tissue cells. Omics and other fields play a very important role. [0003] A common method for animal tissue fixation is to anesthetize the animal to directly obtain the corresponding parts and then fix the corresponding parts. However, for large animals, firstly, it is not easy to obtain a single tissue and fix it in large experimental animals, and discarding other tissues is really a waste of experimental animals. Second, the conventional whole-body sampling of animals cannot achieve temporal synchronization, resultin...

AI Technical Summary

Problems solved by technology

However, for large animals, firstly, it is not easy to obtain a single tissue and fix it in large experimental animals, and discarding other tissues is really a waste of experimental animals

Second, conventional whole-body samples from animals cannot be synchronized in time, resulting in the disappearance of autolyzed proteins in some tissues

Third, incomplete anesthesia in the routine collection of experimental animals can cause stress in the animals, resulting in experimental errors in experimental animal tissues.

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