Device for fixing whole body tissue by perfusion of animal heart

A technology of perfusion fixation and heart, which is applied in the field of experimental animals, can solve the problems of difficult acquisition, animal stress, disappearance of tissue autolytic protein, etc., and achieve the effect of avoiding rupture and high-efficiency synchronous fixation

Pending Publication Date: 2020-01-07
GENERAL HOSPITAL OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for large animals, firstly, it is not easy to obtain a single tissue and fix it in large experimental animals, and discarding other tissues is really a waste of experimental animals
Second, conventional whole-body samples from animals cannot be synchr

Method used

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  • Device for fixing whole body tissue by perfusion of animal heart
  • Device for fixing whole body tissue by perfusion of animal heart

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Experimental program
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Embodiment 1

[0033] In this embodiment, the device for perfusing and fixing whole-body tissue of the animal heart is used to perfuse and fix the whole-body tissue of the animal heart, including: anesthetizing the large animal, measuring the blood pressure of the large animal, performing a thoracotomy to find the heart of the large animal and determining its cardiac cycle System, heart left ventricle cannula and superior vena cava cannula use the device of the present invention and the obtained cardiac extracorporeal power circulation fixation system to perform cardiac perfusion of large animals, perform synchronous fixation of whole body tissues, and obtain materials from animals. Specifically:

[0034] 1. Preparation of reagents for animal heart perfusion

[0035] 1. Cardiac perfusion flushing agent: 150 mg / mL sodium citrate solution, based on 10% to 15% of the body weight of large animals.

[0036] 2. Cardiac perfusion fixative: 4% paraformaldehyde solution, prepared according to the do...

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Abstract

The invention relates to the technical field of experimental animals, and in particular to a device for fixing whole body tissue by perfusion of an animal heart. The device for fixing the whole body tissue by perfusion of the animal heart comprises a pressure pump, a buffer bottle, a washing liquid bottle and a fixing liquid bottle; the pressure pump is connected with the air inlet end of the buffer bottle through an air guide pipe; the air inlet end of the washing liquid bottle and the air inlet end of the fixing liquid bottle are connected with the air outlet end of the buffer bottle separately through corresponding air supply pipes; and the air outlet end of the washing liquid bottle and the air outlet end of the fixing liquid bottle are connected with liquid outlet pipes. By using thedevice, animal whole body tissue cells can be quickly and efficiently fixed synchronously through animal anesthesia and animal thoracotomy; and meanwhile, more complete tissue proteins and nucleic acid molecules can be obtained simultaneously to provide high-quality experimental specimens for later experiments.

Description

technical field [0001] The invention relates to the technical field of experimental animals, in particular to a device for animal heart perfusion and fixation of whole body tissues. Background technique [0002] Animal tissue fixation is the first step in life science research, which provides an experimental basis for qualitative and quantitative analysis of proteins, detection of nucleic acid molecules, and morphological observation of the fine structure of tissue cells. Omics and other fields play a very important role. [0003] A common method for animal tissue fixation is to anesthetize the animal to directly obtain the corresponding parts and then fix the corresponding parts. However, for large animals, firstly, it is not easy to obtain a single tissue and fix it in large experimental animals, and discarding other tissues is really a waste of experimental animals. Second, the conventional whole-body sampling of animals cannot achieve temporal synchronization, resultin...

Claims

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Application Information

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IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 徐良慰杨仕明袁硕龙赵立东丛涛陈伟郭维维
Owner GENERAL HOSPITAL OF PLA
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