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Application of adenosine monophosphate-activated protein kinase (AMPK) inhibitor Compound C to drug for treating tumors

A technology of tumor drugs and inhibitors, applied in the field of medicine, can solve the problems of drug resistance, tumor recurrence, and side effects, and achieve the effect of inhibiting tumor cell activity and induction, promoting tumor cell apoptosis, and reducing harm

Inactive Publication Date: 2020-01-07
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, to provide an application method of an AMPK inhibitor Compound C, to solve the side effects that the current Compound C is only used for specific AMPK inhibitors and the current treatment methods and drugs for cancer Large, prone to technical problems such as drug resistance
[0007] Another object of the present invention is to provide a kind of medicine for treating tumors, to solve the technical problems of adopting existing drugs for treating tumors with toxic and side effects, prone to drug resistance, easy recurrence of tumors, etc.

Method used

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  • Application of adenosine monophosphate-activated protein kinase (AMPK) inhibitor Compound C to drug for treating tumors
  • Application of adenosine monophosphate-activated protein kinase (AMPK) inhibitor Compound C to drug for treating tumors
  • Application of adenosine monophosphate-activated protein kinase (AMPK) inhibitor Compound C to drug for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 11

[0037] Effect of Different Concentrations of Compound C on Viability of Blood Tumor Cells by MTS

[0038] S11: Cell pretreatment

[0039] 1) Two kinds of lymphoma cells (Jurkat, K562) were resuspended to 5×10 4 Cells / mL, seeded in 96-well plates respectively;

[0040] 2) Add Compound C to the 96-well plate respectively, and set the concentration gradient of 0 μM, 0.5 μM, 1 μM, 5 μM, 10 μM and 20 μM, and after culturing for 48 hours, detect the cell survival;

[0041] S12: MTS detection of cell viability

[0042] 1) Gently mix 2mL MTS and 100μL PMS evenly;

[0043] 2) Add 20 μL of MTS mixture to each well, mix thoroughly, place in a carbon dioxide cell incubator, and incubate for 3 hours in the dark;

[0044] 3) Use a microplate reader to detect the wavelengths at OD490nm and OD630nm;

[0045] 4) Using the OD value at the Compound C concentration of 0 μM as a control, analyze the cell viability at each concentration gradient.

[0046] Using Rapamycin reagent as a control,...

Embodiment 12

[0048] Effect of Compound C on tumor cell apoptosis analyzed by flow cytometry

[0049] S11: Cell pretreatment

[0050] 1) Lymphoma cells (Jurkat, K562) were resuspended to 3×10 5 Cells / mL, seeded in 12-well plate;

[0051] 2) After adding Compound C and culturing for 48 hours, detect cell apoptosis;

[0052] S12: Analysis of cell apoptosis by flow cytometry

[0053] 1) Collect the cell suspension;

[0054] 2) Centrifuge the cell suspension at 800 g at 4°C for 5 min, discard the supernatant, and collect the cells in the pellet;

[0055] 3) Wash the cells twice with pre-cooled PBS, and then resuspend the cells with 100 μL 1×AnnexinV Binding Buffer;

[0056] 4) Add 5 μL Annexin V-FITC and 5 μL PI staining solution to each sample, mix gently, and react in the dark at room temperature for 15 minutes; pay attention to setting single staining and blank control, and follow-up flow cytometry adjustment needs to be compensated;

[0057] 5) After the incubation, add 400 μL pre-coo...

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Abstract

The invention discloses novel application of an adenosine monophosphate-activated protein kinase (AMPK) inhibitor, namely a Compound C, and particularly relates to application of the Compound C inhibitor to preparation of a drug for treating tumors. The Compound C can play a role in resisting the blood system tumors by inhibiting the activity of tumor cells and inducing apoptosis of the tumor cells. According to the novel application of the AMPK inhibitor, namely the Compound C, the novel drug for clinically treating the blood system tumors is provided.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of an AMPK inhibitor Compound C in a drug for treating tumors. Background technique [0002] Tumor (tumor, neoplasm) is a non-hereditary genetic disease. Under the action of tumorigenic factors, the genes of normal cells are changed, and the normal regulation of their growth is lost, resulting in abnormal proliferation. Tumor cells have three remarkable basic characteristics: immortality, migration and loss of contact inhibition. The core idea of ​​tumor treatment is to kill and eliminate tumor cells. [0003] One of the mechanism of action of commonly used clinical tumor treatment methods such as chemotherapy, radiotherapy, hormone therapy and some biological therapies is to induce apoptosis of tumor cells. For example, cell apoptosis induced by radiotherapy is mainly mediated by Fas, and some cell cycle-related proteins, Bcl-2 and Bax also play a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/519A61P35/00A61P35/02
CPCA61K31/519A61P35/00A61P35/02
Inventor 万晓春黄诗然章桂忠
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI