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Multi-stage salt stress microalgae culture method

A microalgae culture and salt stress technology, applied in the field of microalgae culture, can solve the problem of damage repair active substances, achieve efficient growth and reduce a large number of deaths

Inactive Publication Date: 2020-01-07
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no public method of using salt stress to gradually stress microalgae in stages to make them produce and accumulate damage repair active substances in large quantities

Method used

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  • Multi-stage salt stress microalgae culture method
  • Multi-stage salt stress microalgae culture method

Examples

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Effect test

Embodiment 1

[0028] Using Chlamydomonas (sp.cw15, purchased from the Algae Bank of Duke University in the United States) cells as algae to carry out a three-stage salt-stressed microalgae cultivation, the specific steps are as follows:

[0029] 1. Obtain the pre-culture solution of Chlamydomonas cells. Prepare 3 L of TAP medium, the specific formula is shown in Table 1 below. After sterilization, Chlamydomonas (sp.cw15) algae cells were inserted into it, and the inoculated culture solution was placed at 25°C, and the intensity of sunlight was 100 μmol / (m 2 · s), cultivated for 7 days in a shaker light incubator with a rotation speed of 20 rpm, to obtain a pre-culture solution of Chlamydomonas cells.

[0030] Table 1 TAP medium formula

[0031]

[0032]

[0033] 2. Detect the concentration of Chlamydomonas cells in the pre-culture solution. After shaking the Chlamydomonas cell pre-culture solution, use a hemocytometer to count under the microscope, and calculate the concentration o...

Embodiment 2

[0040] Chlorella (sp.dh8248, preserved in the laboratory, collected in East Lake, Wuhan) cells were used as algae species to carry out a three-stage salt-stressed microalgae culture. The specific steps are as follows:

[0041] 1. Obtain the chlorella cell preculture solution. Prepare 3 L of general-purpose TAP medium for green algae cultivation, and the formula of TAP medium is shown in Table 1. After sterilization, insert Chlorella cells into it, place the inoculated culture solution at 25°C, and the intensity of sunlight light is 100 μmol / (m 2 s) in a shaker light incubator with a rotating speed of 20rpm, and cultivate for 7 days to obtain the chlorella cell preculture solution.

[0042] 2. Detect the concentration of chlorella cells in the pre-culture solution. After shaking the chlorella cell pre-culture solution, use a hemocytometer to count under a microscope, and calculate the concentration of chlorella cells in the pre-culture solution as 1.0x10 6 a / L.

[0043] 3. ...

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Abstract

The invention provides a multi-stage salt stress microalgae culture method which comprises the following steps of (1) preculturing a microalgae cell culture solution; and (2) performing salt stress processing on the pre-cultured microalgae cell culture solution in a multi-stage progressive way. Microalgae cells are in contact with salt stress culture solutions of different concentrations in a multi-stage and progressive way; the time is provided for the microalgae cells to adapt to the high-intensity salt stress; and the mass production and accumulation of damage repair substances in algae cells are facilitated. In addition, mass mortality of the algae cells due to direct strong salt stress can be greatly reduced. By using the method, the continuous and efficient growth of the microalgae cells under the three kinds of progressive salt stress conditions is realized; the content of superoxide dismutase and polyunsaturated fatty acids in the microalgae cells can be greatly improved; and the method can be widely used for producing and manufacturing damage repair active substances such as superoxide dismutase and polyunsaturated fatty acids.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a multi-stage salt stress microalgae cultivation method. Background technique [0002] As a pioneer species on the earth, microalgae is an autotrophic organism that uses light energy, carbon dioxide and water to synthesize various organic substances in its cells, and has a strong ability to adapt to the environment. This extremely strong adaptability is closely related to the efficient metabolic pathways formed by microalgae cells during the long-term evolution process and the large amount of various damage repair active substances produced and accumulated. [0003] A large number of research reports have pointed out that microalgal cells produce and accumulate superoxide dismutase, polyunsaturated fatty acids, pigments and other substances with damage repair activity under stress culture conditions, which are much higher than normal cultured microalgal...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/36C12R1/89
CPCC12N1/12C12N1/36
Inventor 高翔周维成杨婷
Owner NINGBO UNIV
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