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New acetylesterase, preparation and application of new acetylesterase in accelerating [beta]-mannase for degrading acetylated mannan

A technology of mannanase and acetyl esterase, applied in the field of genetic engineering, can solve the problems such as patent publication documents that have not yet been found, and achieve the effect of improving the degree of degradation

Active Publication Date: 2020-01-07
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • New acetylesterase, preparation and application of new acetylesterase in accelerating [beta]-mannase for degrading acetylated mannan
  • New acetylesterase, preparation and application of new acetylesterase in accelerating [beta]-mannase for degrading acetylated mannan
  • New acetylesterase, preparation and application of new acetylesterase in accelerating [beta]-mannase for degrading acetylated mannan

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Embodiment Construction

[0034] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0035] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0036] An acetyl esterase, the amino acid sequence of which is shown in the sequence SEQ ID NO:1.

[0037] Preferably, the acetyl esterase is from Bacillus alkalophilus N16-5.

[0038] Preferably, the acetyl esterase has the ability to hydrolyze p-nitrophenyl acetate and 4-methylumbelliferyl acetate, and the enzyme activities are 1.531 and 1.679 U / mg respectively.

[0039] Preferably, the acetylesterase has the ability to improve the ability of β-mannanase to degrade acetylmannan.

[0040] An acetyl esterase, the nu...

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Abstract

The invention relates to an acetylesterase of which the amino acid sequence is disclosed in a sequence SEQ ID NO:1. The acetylesterase AesA shows a catalytic activity for various acetylated substrates, the degradation degree of [beta]-mannase for acetylated mannan can be improved, so that the acetylesterase has an application value in an acetylated mannan hydrolysis process, and the acetylesterasecan be applied to an aspect of the degradation of the acetylated mannan by the [beta]-mannase.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a new acetyl esterase, its preparation and its application in promoting the degradation of acetylated mannan by β-mannanase. Background technique [0002] Acetylesterases are a class of enzymes that catalyze the hydrolysis of acetyl ester bonds to produce acetic acid and alcohols. Acetyl esterase, which uses acetylated carbohydrates as a substrate, may also be called acetyl carbohydrate esterase, which is a type of carbohydrate esterase. [0003] Mannan is the second largest hemicellulose after xylan, and it is an important component of lignocellulose, and can also exist in various plant tissues as energy storage carbohydrates. There are often acetyl group substitutions on mannan from natural sources, and specific acetyl esterases can cleave the acetyl groups on these substrates, thereby facilitating the further degradation of mannan substrates by mannanase. The man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/18C12N15/70C12N1/21C12P19/14C12P19/02C12R1/19
CPCC12N9/18C12Y301/01006C12N15/70C12P19/14C12P19/02Y02E50/10
Inventor 宋亚囝马翠萍刘朵朵潘炳菊申会涛罗学刚李中媛张同存
Owner TIANJIN UNIV OF SCI & TECH
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