Biosensor surface modified based on Ti3C2Tx and preparation method and application thereof

A biosensing and sensor-based technology, applied in the field of Ti3C2Tx-based biosensing surface and its preparation, can solve the problems of low adsorption capacity and inability to analyze biomolecular interactions, so as to meet the detection requirements and improve the detection ability , the effect of convenient operation

Inactive Publication Date: 2020-01-10
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing total internal reflection ellipsometry biosensors have high detection sensitivity for biomacromolecules such as proteins, but for small biomolecules such as amino acids, there is a problem that the adsorption capacity is low and effective biomolecular interaction analysis cannot be performed.

Method used

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  • Biosensor surface modified based on Ti3C2Tx and preparation method and application thereof
  • Biosensor surface modified based on Ti3C2Tx and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] This embodiment provides a Ti-based 3 C 2 T X Methods for the preparation of modified biosensing surfaces, such as figure 1 shown, including:

[0026] (1) 1g LiF is completely dissolved in 20ml 9M HCl to prepare etching solution;

[0027] (2) 1g Ti 3 AlC 2 Gradually add to the etching solution, and react at 35°C for 24 hours;

[0028] (3) The resulting acidic product was repeatedly washed with deionized water and centrifuged at 3500rpm for 5 minutes until a stable dark green Ti 3 C 2 T x Sheet suspension. At this point, the pH of the solution is ≥ 6; then add deionized water to the precipitate and shake it by hand for 5 minutes to separate the flakes. After 1 hour of sonication, the resulting solution was centrifuged at 3500 rpm for 1 hour to obtain Ti 3 C 2 T x supernatant;

[0029] (4) Immerse the substrate coated with 50nm gold film into Ti 3 C 2 T x Saturated solution, reacted at 4°C for 60 hours, due to electrostatic interaction, Ti 3 C 2 T x Wi...

Embodiment 2

[0031] This embodiment provides a potential modulation type total internal reflection biosensor, which includes the Ti-based 3 C 2 T x Modified biosensing surfaces.

Embodiment 3

[0033] This example provides a method for detecting arginine using the potential-modulated total internal reflection biosensor obtained in Example 2, specifically: during detection, a voltage of -50mV is applied to the sensing surface to increase the adsorption of arginine on the surface .

[0034] After testing, compared with the traditional arginine detection method, using the method described in Example 3 can increase the detection limit of arginine by 100 times.

[0035] The specific detection principle is as figure 2 shown.

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Abstract

The invention belongs to the technical field of biosensor preparation, and in particular relates to a biosensor surface modified based on Ti3C2Tx, a preparation method and application thereof. The biological sensing surface modified based on the Ti3C2Tx comprises a sensing substrate and the Ti3C2Tx modified on the surface thereof; wherein Tx is surface group O2-, OH-, F-, NH3, NH4+. The transitionmetal carbide Ti3C2Tx coats a biological sensing substrate to form a sensing surface which can be used for adsorbing charged biological small molecules, thus improving the detection capability of thebiological small molecules. The method is convenient to operate, and compared with the sensing surface of the traditional total internal reflection biosensor, the detection limit of arginine is improved by 100 times, and the actual detection requirement can be met.

Description

technical field [0001] The invention belongs to the technical field of biosensor preparation, in particular to a Ti-based 3 C 2 T x Modified biosensing surfaces and methods of preparation and applications thereof. Background technique [0002] The total internal reflection ellipsometry biosensor is an optical biosensor that can be used to study the adsorption behavior of biomolecules on solid surfaces. It uses the changes in optical properties such as spectral absorption, reflection, and refractive index caused by the adsorption of biomolecules on the solid surface as a detection method, and has the advantages of less damage to biological samples and high detection sensitivity. At the same time, because the samples used for detection can usually avoid complicated pre-processing steps, total internal reflection ellipsometry biosensors can be used for real-time, high-throughput sample detection, thus in biomedical research, disease diagnosis, pharmaceuticals, food safety, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/921G01N21/21
CPCC01B32/921C01P2006/40G01N21/211G01N2021/212
Inventor 王晓红胡丙萌刘巍
Owner TSINGHUA UNIV
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