Method for extracting ergosterol from distillers'grains

A technology of ergosterol and extraction method, which is applied in the field of herbal medicine, can solve the problems that the output of ergosterol cannot keep up with the demand, and achieve the effect of saving cost and benefiting the environment

Active Publication Date: 2020-01-10
宿迁医美科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0003] The invention extracts ergosterol from distiller's grains, and the method can solve the problem of disposing of was

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  • Method for extracting ergosterol from distillers'grains
  • Method for extracting ergosterol from distillers'grains
  • Method for extracting ergosterol from distillers'grains

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[0028] Example 1

[0029] A method for extracting ergosterol from distiller’s grains, said extraction method comprising the following steps:

[0030] Saponification: Extract 100 g of dried distiller’s grains with 800 mL of 95% ethanol and 80 mL of saturated potassium hydroxide solution under heating and refluxing twice, each for 2 hours, and then combine the extracts;

[0031] Extraction: first concentrate the extract 4 times under reduced pressure, then extract 3 times with 660 mL of n-hexane, combine the n-hexane layers, and concentrate the n-hexane layer under reduced pressure to obtain 2.5125 g of the yellow solid extraction product, and recover the n-hexane. TLC of the extracted product And HPLC analysis, where: TLC conditions: developing solvent is petroleum ether and ethyl acetate volume ratio of 3:1; HPLC conditions: chromatographic column is normal phase chromatography column Inertsil, mobile phase is n-hexane and isopropanol The volume ratio is 100:1, the flow rate is 1.0m...

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Abstract

The invention relates to a method for extracting ergosterol from distillers'grains, and belongs to the field of botanical drugs. The extraction method comprises the following steps: extracting distillers'grains by using an ethanol-water solution and a potassium hydroxide solution, extracting the obtained extract solution with n-hexane, and separating the obtained extract product by using a silicagel column to obtain ergosterol. Existing industrially extracted ergosterol is directly obtained by extraction from related fungi, leaching and recrystallization. The ergosterol is extracted from distillers' grains, so that the method is beneficial to the environment and saves the cost.

Description

technical field [0001] The invention relates to a method for extracting ergosterol in distiller's grains, which belongs to the field of herbal medicines. Background technique [0002] "Ergosterol", also known as "ergosterol", is one of the steroid compounds. This compound can be used in biochemical research, and some of its effects are the same as vitamin D2. Ergosterol is an important part of the microbial cell membrane, which plays an important role in ensuring the integrity of the cell membrane, the activity of membrane-bound enzymes, the fluidity of the membrane, cell viability, and the transport of cellular substances. It is the precursor for the production of vitamin D2 and is also It is an intermediate in the production of hormone drugs and can be used to produce cortisone. Ergosterol is a plant sterol found in the fungi yeast and ergot, which can be converted into vitamin D2 under ultraviolet light. Studies have shown that ergosterol may have anti-tumor properties,...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 胡晓进彭缨
Owner 宿迁医美科技有限公司
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