Preparation method and application of anti-herpes simplex virus composite IgY antibody
A herpes simplex virus and antibody preparation technology, which is applied in the direction of antiviral agents, antiviral immunoglobulins, chemical instruments and methods, etc., can solve the problems of no effective eradication method and no sustained immune clearance rate, etc. Fast onset and good curative effect
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[0044] The preparation method of the anti-herpes simplex virus complex IgY antibody provided in this embodiment comprises the following steps:
[0045] Step 1: Preparation of immunogen, mix herpes simplex virus type 1 and herpes simplex virus type 2 with Freund's complete adjuvant and Freund's incomplete adjuvant respectively, stir and emulsify to make water-in-oil Freund's incomplete adjuvant Herpes simplex virus immunogen with Freund's complete adjuvant;
[0046] Step 2: Immunization, injecting Freund's complete adjuvant and Freund's incomplete adjuvant immunogen to laying hens;
[0047] Step 3: Preparation of water extract of anti-herpes simplex virus complex IgY antibody, collect the eggs of immunized hens and take the egg yolk liquid, stir the egg yolk liquid with distilled water, adjust the pH and let stand at low temperature overnight, take the supernatant, filter and sterilize , to obtain the anti-herpes simplex virus complex IgY antibody water extract.
[0048] In s...
Embodiment 1
[0055] Preparation of 100 grams of anti-herpes simplex virus complex IgY antibody water extract production process:
[0056] Step 1: Preparation of Immunogen
[0057] (1) 50 parts of herpes simplex virus type 1 and 50 parts of herpes simplex virus type 2 were mixed and treated with carbolic acid inactivation;
[0058] (2) Mix 200ml of virus liquid with 200ml of Freund's complete adjuvant, use a high-speed mixer to stir and emulsify, and make water-in-oil Freund's complete adjuvant immunogen;
[0059] (3) The preparation of immunogen with Freund's incomplete adjuvant is as follows: mix 200ml of virus liquid with 200ml of Freund's incomplete adjuvant, stir and emulsify with a high-speed mixer, and make water-in-oil Freund's incomplete adjuvant immunogen;
[0060] Step 2: Immunization of laying hens
[0061] (1) Subcutaneously and intramuscularly inject 60 points of Freund's complete adjuvant immunogen into 20-week-old Hy-Line brown laying hens with a syringe, each injecting 1...
Embodiment 2
[0072] Prepare 100 grams of anti-herpes simplex virus compound IgY antibody pure product production process:
[0073] Step 1: Preparation of Immunogen
[0074] (1) 50 parts of herpes simplex virus type 1 and 50 parts of herpes simplex virus type 2 were mixed and treated with carbolic acid inactivation;
[0075] (2) Mix 200ml of virus liquid with 200ml of Freund's complete adjuvant, use a high-speed mixer to stir and emulsify, and make water-in-oil Freund's complete adjuvant immunogen;
[0076] (3) The preparation of immunogen with Freund's incomplete adjuvant is as follows: mix 200ml of virus liquid with 200ml of Freund's incomplete adjuvant, stir and emulsify with a high-speed mixer, and make water-in-oil Freund's incomplete adjuvant immunogen;
[0077] Step 2: Immunization of laying hens
[0078] (1) Subcutaneously and intramuscularly inject 60 points of Freund's complete adjuvant immunogen into 20-week-old Hy-Line brown laying hens with a syringe, each injecting 1.2ml of...
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