Preparation method and application of anti-herpes simplex virus composite IgY antibody

A herpes simplex virus and antibody preparation technology, which is applied in the direction of antiviral agents, antiviral immunoglobulins, chemical instruments and methods, etc., can solve the problems of no effective eradication method and no sustained immune clearance rate, etc. Fast onset and good curative effect

Active Publication Date: 2020-01-10
南京道大药业有限公司
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  • Claims
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Problems solved by technology

And there is no effective eradication method, especially the repeated v

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  • Preparation method and application of anti-herpes simplex virus composite IgY antibody
  • Preparation method and application of anti-herpes simplex virus composite IgY antibody
  • Preparation method and application of anti-herpes simplex virus composite IgY antibody

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preparation example Construction

[0044] The preparation method of the anti-herpes simplex virus complex IgY antibody provided in this embodiment comprises the following steps:

[0045] Step 1: Preparation of immunogen, mix herpes simplex virus type 1 and herpes simplex virus type 2 with Freund's complete adjuvant and Freund's incomplete adjuvant respectively, stir and emulsify to make water-in-oil Freund's incomplete adjuvant Herpes simplex virus immunogen with Freund's complete adjuvant;

[0046] Step 2: Immunization, injecting Freund's complete adjuvant and Freund's incomplete adjuvant immunogen to laying hens;

[0047] Step 3: Preparation of water extract of anti-herpes simplex virus complex IgY antibody, collect the eggs of immunized hens and take the egg yolk liquid, stir the egg yolk liquid with distilled water, adjust the pH and let stand at low temperature overnight, take the supernatant, filter and sterilize , to obtain the anti-herpes simplex virus complex IgY antibody water extract.

[0048] In s...

Embodiment 1

[0055] Preparation of 100 grams of anti-herpes simplex virus complex IgY antibody water extract production process:

[0056] Step 1: Preparation of Immunogen

[0057] (1) 50 parts of herpes simplex virus type 1 and 50 parts of herpes simplex virus type 2 were mixed and treated with carbolic acid inactivation;

[0058] (2) Mix 200ml of virus liquid with 200ml of Freund's complete adjuvant, use a high-speed mixer to stir and emulsify, and make water-in-oil Freund's complete adjuvant immunogen;

[0059] (3) The preparation of immunogen with Freund's incomplete adjuvant is as follows: mix 200ml of virus liquid with 200ml of Freund's incomplete adjuvant, stir and emulsify with a high-speed mixer, and make water-in-oil Freund's incomplete adjuvant immunogen;

[0060] Step 2: Immunization of laying hens

[0061] (1) Subcutaneously and intramuscularly inject 60 points of Freund's complete adjuvant immunogen into 20-week-old Hy-Line brown laying hens with a syringe, each injecting 1...

Embodiment 2

[0072] Prepare 100 grams of anti-herpes simplex virus compound IgY antibody pure product production process:

[0073] Step 1: Preparation of Immunogen

[0074] (1) 50 parts of herpes simplex virus type 1 and 50 parts of herpes simplex virus type 2 were mixed and treated with carbolic acid inactivation;

[0075] (2) Mix 200ml of virus liquid with 200ml of Freund's complete adjuvant, use a high-speed mixer to stir and emulsify, and make water-in-oil Freund's complete adjuvant immunogen;

[0076] (3) The preparation of immunogen with Freund's incomplete adjuvant is as follows: mix 200ml of virus liquid with 200ml of Freund's incomplete adjuvant, stir and emulsify with a high-speed mixer, and make water-in-oil Freund's incomplete adjuvant immunogen;

[0077] Step 2: Immunization of laying hens

[0078] (1) Subcutaneously and intramuscularly inject 60 points of Freund's complete adjuvant immunogen into 20-week-old Hy-Line brown laying hens with a syringe, each injecting 1.2ml of...

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Abstract

The invention discloses a preparation method and application of an anti-herpes simplex virus complex IgY antibody, and belongs to the technical field of herpes simplex virus resisting. The preparationmethod includes the following steps that step 1, an immunogen is prepared, specifically, a herpes simplex virus type 1 and a herpes simplex virus type 2 are taken and mixed and then the mixture is mixed with a Freund's complete adjuvant and a Freund's incomplete adjuvant separately and stirred and emulsified to prepare a water-in-oil Freund's complete adjuvant and Freund's incomplete adjuvant herpes simplex virus immunogen; and step 2, immunity is performed, specifically, the Freund's complete adjuvant immunogen is injected into laying hens. Application to ocular conjunctiva, a nasal cavity and an oral cavity is achieved, no limitations and side effects of systemic medication exist, the local application taking effect is quick, the dosage is low, the curative effect is good, the normal micro-ecological environment on the surface of the conjunctiva, the nasal cavity and the oral cavity is not absorbed and damaged, no toxic and side effects exist, and diseases of herpes labialis, dermatitis herpetiformis and herpes zoster caused by the herpes simplex virus type 1 and the herpes simplex virus type 2 and viruses with a common antigen with the herpes simplex virus type 1 and the herpessimplex virus type 2 are prevented and treated.

Description

technical field [0001] The invention relates to the preparation and application of compound IgY, in particular to a preparation method and application of an anti-herpes simplex virus compound IgY antibody, belonging to the technical field of anti-herpes simplex virus. Background technique [0002] Herpes simplex virus, HSV and Varicella-zoster virus, VZV belong to the alpha herpes virus subfamily Herpesviridae, which are characterized by rapid proliferation, causing cell ballooning and ulceration lesions. Herpes simplex virus is divided into herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) according to the serological response characteristics. Varicella-zoster virus VZV has only one serotype. HSV is spherical, and the complete virus consists of core, The core of the capsid, envelope, and capsule consists of double-stranded DNA, which is wound into a filament reel. The HSV capsid is icosahedral, composed of 162 shell particles, and has a diameter of...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/02A61P31/22
CPCC07K16/087C07K16/02A61P31/22
Inventor 付宇
Owner 南京道大药业有限公司
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