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Detection method for epimedium herb constituent, method for identifying epimedium herb types and application

A detection method, the technology of epimedium, is applied in the direction of measuring device, material separation, analysis of materials, etc., to achieve the effect of low detection cost and simple detection method

Inactive Publication Date: 2020-01-10
劲牌持正堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there is currently a lack of a method that can comprehensively detect the active ingredients in Epimedium medicinal materials and a method for identifying various Epimedium medicinal materials. The Epimedium variety approach is currently needed

Method used

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  • Detection method for epimedium herb constituent, method for identifying epimedium herb types and application
  • Detection method for epimedium herb constituent, method for identifying epimedium herb types and application
  • Detection method for epimedium herb constituent, method for identifying epimedium herb types and application

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Embodiment 1

[0068] The present embodiment provides a kind of detection method of epimedium composition, comprises the steps:

[0069] (1) Preparation of the test solution: Weigh 1.0 g of Epimedium medicinal material (through No. 3 sieve), accurately weigh it, put it into a stoppered Erlenmeyer flask, accurately add 100 mL of 50% ethanol, weigh it, and ultrasound for 1 hour , then weighed, supplemented with 50% ethanol to reduce the weight of the arrow, shaken, filtered through a 0.22 micron organic phase microporous membrane, and took the filtrate to obtain final product.

[0070](2) Preparation of standard solution: Accurately weigh epimedin A, epimedin B, epimedin C, icariin, 2”-O-rhamnosyl icariside II, and Baohuo Glycoside Ⅰ 10.56mg, 9.78mg, 10.60mg, 17.03mg, 6.34mg, 8.75mg in 10mL volumetric flask, dissolved in methanol and dilute to the mark, used as standard stock solution, epimedin A, epimedin B , epimedin C, icariin, 2”-O-rhamnosyl icariside II, and baohuoside I were 1034.88mg / L...

Embodiment 2

[0099] The present embodiment provides a kind of method for distinguishing Epimedium species, get Epimedium cordifolia, Epimedium sagitta, Epimedium vellus, Epimedium Korean and Epimedium Wushan of known varieties, respectively according to The method provided in Example 1 identifies the contents of epimedin A, epimedin B, epimedin C, icariin, 2"-O-rhamnosyl icariside II and baohuoside I , its chromatogram is as Figure 7 with Figure 8 Shown: Figure 7 The Epimedium species in the middle chromatogram from bottom to top are Epimedium Heart Leaf, Epimedium Sagittarius, Epimedium Pili, Epimedium Korean, Epimedium Wushan; Figure 8 The origins of epimedium in the chromatogram from bottom to top are Gansu Longxi, Shaanxi Ningqiang, Ningxia, Chongqing Fengdu, Liaoning Hengren, Northeast China, North Korea, Guizhou, Hubei Xiangyang, Hubei Fangxian, Hubei Enshi, Sichuan Bazhong, Sichuan Nanchong, Sichuan Guangyuan, Sichuan Wangcang) count the content of Epimedium cordifolia, Epime...

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Abstract

The invention provides a detection method for epimedium herb constituent, a method for identifying epimedium herb types and application, and relates to the technical field of medicine analysis. The detection method comprises the steps of eluting a test solution in a mobile phase, and performing chromatographic analysis, wherein the mobile phase comprises an acetonitrile and water mixed system; andeluting according to an optimized elution condition. By the detection method, the following six constituents comprising bepimedin A, bepimedin B, bepimedin C, icariin, 2''-O-rhamnosyl icarisid II andbaohuoside I can be simultaneously detected under the same condition, the technical problem of single constituent in detection of an epimedium herb medicine in the prior art is reduced, and the detection method has the advantages of simplicity, relatively low detection cost and high detection accuracy.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for detecting components of Epimedium and a method for identifying varieties of Epimedium and its application. Background technique [0002] Epimedium is a perennial herb plant with a plant height of 20-60 cm, thick and short rhizomes, dark brown, two times and three compound leaves, basal and cauline, with long stalks, leaflets papery or thick papery, leaf margins with Spiny teeth, white or light yellow flowers, flowering period May-June, fruit period June-August. Epimedium grows in the undergrowth, ditch bushes or damp places on hillsides, at an altitude of 650-3500 meters. It is cultivated in Shaanxi, Gansu, Shanxi, Henan, Qinghai, Hubei, Sichuan and other regions of China. [0003] The whole herb of Epimedium is used medicinally. Indications impotence and premature ejaculation, backache and leg pain, numbness of limbs, hemiplegia, neurasthenia, forgetfulness...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34
Inventor 王玉张继斌陈志元李昌瑜陈搏严书超
Owner 劲牌持正堂药业有限公司