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Method for extracting endocrine disrupters

An endocrine disrupting compound and extraction method technology, applied in the field of endocrine disrupting compound extraction, can solve the problems of unfavorable collection, incomplete extraction of endocrine disrupting compound, inconvenient operation and the like

Inactive Publication Date: 2020-01-14
深圳市龙华区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting endocrine disruptors in order to solve the problem of incomplete extraction of endocrine disruptors, inconvenient operation and unfavorable collection

Method used

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  • Method for extracting endocrine disrupters
  • Method for extracting endocrine disrupters
  • Method for extracting endocrine disrupters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment one, with reference to Figure 1-5 , a method for extracting endocrine disruptors, comprising the steps of:

[0044] S1. Obtain the substitute; pulverize the sample containing the environmental endocrine disruptor, and fully dissolve it with water;

[0045] S2. Preliminary salting-out extraction: Ultrasonic extraction of endocrine disruptors using water-miscible organic solvents and sodium salts to obtain initial salting-out extracts;

[0046] S3, subcritical water extraction for the second extraction; using subcritical water extraction to obtain the subcritical water extraction for the second extraction;

[0047] S4, the extraction mixture is eluted; after the salting-out primary extract and the subcritical water extraction secondary extract are mixed, the eluent is used for elution treatment;

[0048] S5, post-elution treatment, concentration to obtain the extract of endocrine disruptors.

[0049] Wherein, the preliminary extraction of salting out in the...

Embodiment 2

[0064] Embodiment two; the difference between this embodiment and embodiment one is;

[0065] The sodium salt is sodium sulfate, the modifier is a mixture of acetone and ethanol, the subcritical water extraction temperature in S303 is controlled at 110°C, the pressure is controlled at 12MPa, and the pH is controlled at 12; the S402 acetonitrile aqueous solution is eluted Extract the mixture, the concentration of acetonitrile eluting solution is 1:60;

Embodiment 3

[0066] Embodiment three; the difference between this embodiment and embodiment one is;

[0067] The sodium salt is sodium sulfate, the modifier is a mixture of acetone and methanol, the subcritical water extraction temperature in S303 is controlled at 120°C, the pressure is controlled at 12MPa, and the pH is controlled at 12; the S402 acetonitrile aqueous solution is eluted The mixture was extracted, and the concentration of acetonitrile elution solution was 1:80.

[0068] Working principle: the present invention provides an extraction method of endocrine disruptors, covering sampling, sample processing, sample extraction, sample elution extraction, convenient, safe and effective extraction of endocrine disruptors, since the present invention directly processes the samples, the extracted endocrine Interfering substances are clean and thorough; in the preliminary extraction of medium salting out described in the present invention, organic solvent and sodium salt are added, and ...

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Abstract

The invention discloses a method for extracting endocrine disrupters. The method includes the following steps: S1, acquiring a to-be-extracted sample; S2, performing salting-out preliminary extraction; S3, performing subcritical water extraction for secondary extraction; S4, performing elution on the extracted mixture; and S5, performing treatment after elution. The method for extracting the endocrine disruptors includes sampling, sample treatment, sample extraction and sample elution extraction so that the endocrine disruptors are extracted conveniently, safely and effectively, and since thesample is treated directly, the extracted endocrine disruptors are clean and thorough; during salting-out preliminary extraction, an organic solvent and a sodium salt are added, extraction residues are obtained through ultrasonic extraction and centrifugation, and preliminary extraction is completed effectively; furthermore, during subcritical water extraction for secondary extraction, the extraction residues and diatomaceous earth are mixed and treated, a subcritical water extractant is prepared at the same time, and subcritical water extraction is performed; and the extraction rate is increased through control on the temperature and pressure, the temperature is controlled between 100 and 120 DEG C, and the pressure is controlled between 10 MPa and 15 MPa.

Description

technical field [0001] The invention belongs to the field of extraction of endocrine disruptors, in particular to an extraction method of endocrine disruptors. Background technique [0002] Endocrine disruptors, also known as environmental hormones, are exogenous chemical substances that interfere with the endocrine system. Various pathways do not directly bring abnormal effects on organisms as toxic substances, but act on organisms similarly to estrogen. Even if the amount is very small, they can also cause endocrine imbalances in organisms and various abnormal phenomena. Such substances can cause genital disturbances, abnormal behavior, reduced reproductive capacity, larval death, and even extinction in animals and humans. [0003] Hormones have a role in the maintenance of internal environment stability and the regulation of developmental processes. Endocrine disruptors are exogenous factors that interfere with the manufacture, release, transport, metabolism, binding, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02
CPCB01D11/0265B01D11/0284
Inventor 周雪贞宁洁聂芙蓉黎清现谢小红蔡伟奕
Owner 深圳市龙华区中心医院