Preparation method of novel cell additive and novel cell additive product

An additive and cell technology, applied in the field of preparation of new cell additives, can solve the problems of easy precipitation, toxicity, and difficulty in dissolving, and achieve the effects of no solid precipitation, accurate concentration, and easy storage.

Pending Publication Date: 2020-01-14
郭永珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, the purpose of this patent is to provide a ready-to-use stearic acid, sodium stearate cell additive and a preparation method thereof for the current technical blank in this field. There are technical problems such as solvent t...

Method used

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  • Preparation method of novel cell additive and novel cell additive product
  • Preparation method of novel cell additive and novel cell additive product
  • Preparation method of novel cell additive and novel cell additive product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of a novel cell supplement, the specific steps are as follows:

[0030] step one)

[0031] The stearic acid component solution dissolved at high temperature and the BSA component solution dissolved at room temperature are respectively prepared, and the order of preparation is not specific. The stearic acid component solution contains stearic acid, and the BSA component solution contains degreasing BSA.

[0032] Among them, the configuration of the stearic acid component solution dissolved at high temperature includes the following specific process: take the buffer solution in a glass centrifuge tube, use an analytical balance to weigh the stearic acid, pour it into the glass centrifuge tube, and place it in a 70-100°C water bath. After the stearic acid is completely dissolved in the buffer, the high temperature of 70-100°C is maintained. It should be noted that a heating system such as a water bath or other metal bath can be used.

[0033] The pr...

Embodiment 2

[0042] A preparation method of a novel cell supplement, the specific steps are as follows:

[0043] step one)

[0044] The stearic acid component solution dissolved at high temperature and the BSA component solution dissolved at room temperature are respectively prepared, and the order of preparation is not specific. Wherein, the stearic acid component solution contains sodium stearate, and the BSA component solution dissolves and contains non-fat BSA.

[0045] Among them, the configuration of the stearic acid component solution dissolved at high temperature includes the following specific process: take the buffer solution in a glass centrifuge tube, use an analytical balance to weigh the sodium stearate, pour it into the glass centrifuge tube, and place it in a 70-100°C water bath Wait until it is completely dissolved in the pot, and after the sodium stearate is completely dissolved in the buffer, maintain a high temperature of 70-100 °C. It should be noted that a heating s...

Embodiment 3

[0054] A preparation method of a novel cell supplement, the specific steps are as follows:

[0055] step one)

[0056] The stearic acid component solution dissolved at high temperature and the BSA component solution dissolved at room temperature are respectively prepared, and the order of preparation is not specific. The stearic acid component solution contains sodium stearate, and the BSA component solution contains low-fat BSA.

[0057] Among them, the configuration of the stearic acid component solution dissolved at high temperature includes the following specific process: take the buffer solution in a glass centrifuge tube, use an analytical balance to weigh the sodium stearate, pour it into the glass centrifuge tube, and place it in an 80°C water bath. After it is completely dissolved, after the sodium stearate is completely dissolved in the buffer, the high temperature of 80°C is maintained. It should be noted that a heating system such as a water bath or other metal b...

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Abstract

The invention provides a preparation method of a novel cell additive and a novel cell additive product, and relates to the field of in vitro cell research for dyslipidemia relevant diseases. The method comprises the steps of adding a stearic acid component solution obtained by high-temperature dissolving to a BSA component solution obtained by room-temperature dissolving, performing shaking for uniform mixing, and after sufficient shaking combination, performing filtering to remove bacteria to obtain the novel cell additive product. A preparation method provided by the invention can solve thetechnical problems that in the prior art, in the preparation process of stearic acid or sodium stearate, solvent toxicity, difficult dissolving, easy separation and the like exist, and has the advantages that the concentration of the stearic acid or the sodium stearate is precise and controllable, products are good in stability, easy to store and the like, and the produced novel cell additive belongs to a ready-to-use product.

Description

technical field [0001] The invention relates to the field of in vitro cell research of dyslipidemia-related diseases, in particular to a preparation method of a novel cell additive and a product thereof. Background technique [0002] In recent years, the development of my country's economy has prompted the dietary structure of residents to change from low-fat to high-fat patterns. According to the "Survey on Nutrition and Chronic Disease Status of Chinese Residents (2015)", the prevalence of dyslipidemia among Chinese adults is as high as 39.9-40.8%. Dyslipidemia is mainly manifested as hypertriglyceridemia and free fatty acidemia, causing chronic damage to tissues and organs, leading to the occurrence of dyslipidemia-related diseases such as type 2 diabetes, obesity, and fatty liver. This phenomenon is called lipotoxicity. [0003] In in vitro studies, high concentrations of saturated free fatty acids are often added to the cell culture medium in order to simulate cell dama...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0018C12N2500/30C12N2501/998
Inventor 闫文俊郭永珍夏云龙陶凌
Owner 郭永珍
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