Preparation and application of exosome with efficacy of hair growth promoting

A technology of exosomes and efficacy, applied in the field of regenerative medicine and biology, can solve the problems of not being able to store and transport for a long time, affecting the effect of use, causing tumors, etc., and achieve the effect of convenient use, simple extraction process and good curative effect

Inactive Publication Date: 2020-01-17
陕西中鸿科瑞再生医学研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of stem cell suspension needs to be used and prepared immediately, and cannot be stored and transported for a long time, and there are strict requirements on the quantity and state of the injected cells, otherwise it will affect the final use effect, so there are certain restrictions on use.
In order to solve the above problems and overcome the hidden dangers of tumorigenicity in the

Method used

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  • Preparation and application of exosome with efficacy of hair growth promoting
  • Preparation and application of exosome with efficacy of hair growth promoting
  • Preparation and application of exosome with efficacy of hair growth promoting

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Isolation and culture of umbilical cord mesenchymal stem cells

[0034] 1. Organization reception: After consulting and agreeing, sign the "Informed Letter and Consent Letter" with the baby's family members, aseptically collect the umbilical cord of the healthy full-term baby, after the two ends of the umbilical cord are ligated, quickly place it in a sterile storage and transportation solution, and seal it Save and deliver to the laboratory as soon as possible within 1 hour;

[0035] 2. Tissue processing: In the ultra-clean workbench, take out the received umbilical cord tissue, put it into a petri dish, and measure the length and width of the tissue. After the measurement, wash with normal saline twice, then cut the umbilical cord into small pieces of about 3 cm with sterile scissors, cut the umbilical cord along the growth direction of the blood vessels in the umbilical cord, remove the umbilical vein and umbilical artery, and obtain Wharton's jelly;

[0...

Embodiment 2

[0043] Example 2: Treatment of hair loss with exosomes derived from umbilical cord mesenchymal stem cells

[0044] Alopecia patients were recruited for volunteer verification, twice a month with an interval of 15 days. Before injection, the exosome injection solution stored in low temperature was taken out and rewarmed. Volunteers took pictures of the affected area, and then wiped the affected area with iodophor twice, then dipped a cotton swab in saline and wiped it twice. Inject multiple subcutaneous injections around the affected area. Note: Do not wash your hair or take a bath within 24 hours after the injection, and do not touch the injection site with irritating substances.

[0045] Results: Volunteer verifiers had different effects after injection, new hair grew in the affected area, androgenetic alopecia patients had more hair in the affected area after injection, and alopecia areata patients reduced the area of ​​the affected area ( image 3 ).

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Abstract

The invention belongs to the technical field of regenerative medicine and biology and particularly relates to preparation and application of exosome with an efficacy of hair growth promoting. The exosome with the efficacy of hair growth promoting is prepared by steps of selecting human umbilical cord mesenchymal stem cells as an exosome source, culturing, collecting cell culture fluid and performing gradient centrifugal extraction and is rich in various physiological activators such as RNA and various proteins and capable of effectively improving hair loss of patients by subcutaneous injection.

Description

technical field [0001] The invention belongs to the technical field of regenerative medicine and biology, and in particular relates to the preparation and application of exosomes with hair growth effect. Background technique [0002] In recent years, the number of people with hair loss has shown a linear upward trend, regardless of gender, age and industry. The latest data shows that there are 250 million people with hair loss in my country, and there is one out of every six people on average. Androgenetic alopecia (AR) is a common type of hair loss and a hormone-dependent alopecia. The hair shaft becomes thinner and yellower, and the hair thins and falls out. Minoxidil and finasteride are commonly used clinically for the treatment of AR. Alopecia areata, commonly known as "ghost shaved head", is a sudden localized hair loss. The specific cause is unknown, but researchers generally believe that it is an autoimmune disease with genetic predisposition and environmental trigg...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/08A61P17/14C12N5/0775
CPCA61K9/0019A61K9/08A61K35/28A61P17/14C12N5/0665C12N2509/00
Inventor 董玲娟明磊国王花
Owner 陕西中鸿科瑞再生医学研究院有限公司
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