Application of neuronal guidance factor Sema to preparation of injection for treating osteoarthritis

A technology for guiding factors and osteoarthritis, applied in the directions of anti-inflammatory agents, bone diseases, non-central analgesics, etc., can solve problems such as the application of Sema macromolecular protein injection in bone joints that have not been disclosed, and achieve slowing down the progression of osteoarthritis, Small injection volume, less swelling and less discomfort

Active Publication Date: 2020-01-17
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the pharmaceutical preparations described in the present invention are different from the disease types used in the existing preparations, and the prior art has never disclosed the application of injections prepared from Sema macromolecular proteins as active ingredients in the treatment of bone and joints.

Method used

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  • Application of neuronal guidance factor Sema to preparation of injection for treating osteoarthritis
  • Application of neuronal guidance factor Sema to preparation of injection for treating osteoarthritis
  • Application of neuronal guidance factor Sema to preparation of injection for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1 injection

[0057] S1: Prepare solution, take 100μg of Sema 3a protein (1mg / ml) and dissolve it in 19.9mL of phosphate buffer solution to prepare a drug solution with a drug concentration of 5μg / mL;

[0058] S2: Activated carbon treatment, adding 0.2% of the total amount of the solution to the medicinal solution and treating it with activated carbon for 0.5 hours;

[0059] S3: initial filtration, filter the liquid medicine with filter paper;

[0060] S4: Sterilize by filtration, use a microporous filter membrane with a pore size of 0.22 μm to sterilize the medicinal solution and remove the heat source;

[0061] S5: Subpackage the medicinal solution in units of 2 mL, fill and seal to obtain an injection solution.

Embodiment 2

[0062] The preparation of embodiment 2 injection

[0063] The preparation method and raw materials are the same as those in Example 1, except for S1. 140 μg of Sema 3a protein (1 mg / ml) was dissolved in 19.86 mL of phosphate buffer solution to prepare a drug solution with a drug concentration of 7 μg / mL.

Embodiment 3

[0064] The preparation of embodiment 3 injections

[0065] The preparation method and raw materials are the same as those in Example 1, except for S1. 200 μg of Sema 3a protein (1 mg / ml) was dissolved in 19.8 mL of phosphate buffer solution to prepare a drug solution with a drug concentration of 10 μg / mL.

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Abstract

The invention discloses application of a neuronal guidance factor Sema to preparation of an injection for treating osteoarthritis. The neuronal guidance factor Sema is selected from a Sema 3a proteinwhich can be selected from a natural protein or a recombinant protein. On the other hand, the invention discloses the injection based on the neuronal guidance factor Sema and a preparation method of the injection. The injection is prepared from the neuronal guidance factor Sema and a phosphate buffer solution, and the concentration of the neuronal guidance factor Sema in the phosphate buffer solution is 5-10 [mu]g / mL. It is demonstrated through clinical trials that the prepared injection has a good treating effect on patients with early degenerative osteoarthritis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of a nerve guiding factor Sema macromolecular protein in preparing an injection for treating osteoarthritis. Background technique [0002] Osteoarthritis (OA) refers to a synovial joint disease characterized by cartilage loss and periarticular bone reaction, also known as osteoarthritis, degenerative osteoarthritis, hypertrophic or hypertrophic arthritis, etc. Most common rheumatic disease worldwide. Osteoarthritis is the degenerative injury of articular cartilage, reactive hyperplasia of joint margins and subchondral bone caused by aging, obesity, strain, trauma, congenital abnormalities of joints, joint deformities and many other factors. Osteoarthritis is the most common joint disease among middle-aged and elderly people. According to survey research, the prevalence rate of people over 60 years old can reach 50%, and that of people over 75 years old can reach 80...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/08A61P19/02A61P19/08A61P19/04A61P29/00
CPCA61K38/1709A61K9/08A61K9/0019A61P19/02A61P19/08A61P19/04A61P29/00Y02A50/30
Inventor 黄石书杨帆龙丹高大双吴迪伟尤炫合
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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