A kind of preparation method of trehalose
A trehalose and inoculum technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low trehalose accumulation and affect the trehalose production, so as to increase the trehalose accumulation Effect
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Embodiment 1
[0046] The preparation of embodiment 1 trehalose
[0047] (1) Inoculate honey zygosaccharomyces (Zygosaecharomyces mellis) LGL-2 inoculum solution (the inoculum size is 10% of the weight of the culture solution) in a culture solution with a sugar concentration of 400g / L, and ferment for 96h at 24°C to obtain a fermentation solution ;
[0048] The inoculum was prepared by the following method: take 2 rings of activated Zygosaecharomyces mellis LGL-2 and add it to 10 mL of 400 g / L culture solution with a sugar concentration of 400 g / L, and cultivate it at a constant temperature at 28 ° C for 48 hours, and then add it to Continue culturing at 28°C for 48 hours in 90 mL of culture solution with a sugar concentration of 400 g / L to obtain the inoculum;
[0049] (2) Centrifuge the fermentation broth to obtain yeast sludge, then put the yeast sludge in a 90°C oven for 60 minutes, add deionized water at a solid-to-liquid mass volume ratio of 1g: 10mL, and centrifuge in a water bath at...
Embodiment 2
[0051] The preparation of embodiment 2 trehalose
[0052] (1) Inoculate honey zygosaccharomyces (Zygosaecharomyces mellis) LGL-2 inoculum solution (the inoculum size is 12% of the weight of the culture solution) in a culture solution with a sugar concentration of 300g / L, and ferment at 28°C for 84h to obtain a fermentation solution ;
[0053] The inoculum was prepared by the following method: take 2 rings of activated Zygosaecharomyces mellis LGL-2 and add it to 10 mL of 400 g / L culture solution with a sugar concentration of 400 g / L, and cultivate it at 28 ° C for 48 hours, then pour it into Continue culturing at 28°C for 48 hours in 90 mL of culture solution with a sugar concentration of 400 g / L to obtain the inoculum;
[0054] (2) Centrifuge the fermentation broth to obtain yeast sludge, then put the yeast sludge in a 90°C oven for 60 minutes, add deionized water at a solid-to-liquid mass volume ratio of 1g: 10mL, and centrifuge in a water bath at 42°C for 20 minutes , Tak...
Embodiment 3
[0056] The preparation of embodiment 3 trehalose
[0057] (1) Inoculate honey zygosaccharomyces (Zygosaecharomyces mellis) LGL-2 inoculum solution (the inoculum size is 8% of the weight of the culture solution) in the culture solution with a sugar concentration of 500g / L, and ferment at 20°C for 108h to obtain a fermentation solution ;
[0058] The inoculum was prepared by the following method: take 2 rings of activated Zygosaecharomyces mellis LGL-2 and add it to 10 mL of 400 g / L culture solution with a sugar concentration of 400 g / L, and cultivate it at 28 ° C for 48 hours, then pour it into Continue culturing at 28°C for 48 hours in 90 mL of culture solution with a sugar concentration of 400 g / L to obtain the inoculum;
[0059] (2) Centrifuge the fermentation broth to obtain yeast sludge, then put the yeast sludge in a 90°C oven for 60 minutes, add deionized water at a solid-to-liquid mass volume ratio of 1g: 10mL, and centrifuge in a water bath at 42°C for 20 minutes , T...
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