A method to improve the fertilization rate of zebrafish

A zebrafish, fertilization rate technology, applied in fish farming, pharmaceutical formulations, medical preparations containing active ingredients, etc., to reduce irritation, promote sperm quality improvement, and promote gonadal development.

Active Publication Date: 2021-08-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many factors that affect the quality of zebrafish eggs and the fertilization rate, and there is still a lot of room for improvement in the method of using melatonin alone to improve the fertilization rate

Method used

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  • A method to improve the fertilization rate of zebrafish
  • A method to improve the fertilization rate of zebrafish
  • A method to improve the fertilization rate of zebrafish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Such as figure 1 Shown, the breeding box that the application can make fish roe separation used is upper casing 1, middle channel 2 and lower casing 3 successively from top to bottom, for the convenience of processing and placing, upper casing, lower casing and and The middle channels are each provided with a circular cross section. The upper box is provided with a water inlet 11, and the junction of the middle channel and the upper box is provided with partitions 4 that are arranged side by side to separate the upper box and the middle channel, and intervals are provided between adjacent partitions, which can The zebrafish broodstock that blocked the upper box passed into the middle channel, but allowed fish eggs to enter the middle channel from the upper box. Such as figure 2 As shown, the dividing plate is positioned on the surface of the upper casing and is provided with a circular arch diversion slope 41, and its cross section is circular arched, which can preve...

Embodiment 2

[0033] The breeding box of the present embodiment has been further modified on the breeding box of embodiment 1, as image 3 As shown, the dividing plate is located on the surface of the upper box to set a triangular diversion slope 41 ', of course, at the dividing plate next to the side of the upper box, an inclined slope is still used. At the same time, an air inlet pipe and an air pump are also configured, the air inlet pipe is connected with the air pump, the air outlet port of the air inlet pipe is located above the upper box body, and a shower head 12 is arranged at the air outlet port of the air inlet pipe, and the shower head extends into the water surface of the upper box body Ventilate downwards.

Embodiment 3

[0035] A method for improving the fertilization rate of zebrafish, comprising the steps of:

[0036] (1) The 10-week-old female zebrafish and the male zebrafish are separately cultivated for routine nutrition enhancement, and the light time is extended to 12 hours / day, and the light intensity is 100lx, and the remaining time is kept in a dark state;

[0037] (2) At the age of 20 weeks, female zebrafish and male zebrafish are added to the breeding box that can separate fish eggs according to the ratio of 1:2, and melatonin is added to the water body of the breeding box to maintain melatonin in the water body. The element concentration is 5μg / L;

[0038] (3) After the dark state was switched to light for 2 hours, the water temperature was rapidly raised by 1°C within 30 minutes and kept for 2 hours. The male zebrafish chased and stimulated the female zebrafish to lay eggs, and then returned to the water temperature;

[0039] (4) Fish eggs are separated from broodstock, and the ...

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Abstract

The present invention relates to the technical field of aquatic animal reproduction and breeding. In order to further improve the fertilization rate of zebrafish, the team of technical personnel of the application conducts in-depth research on the basis of previous research, and provides a method for improving the fertilization rate of zebrafish. Melanin prevents egg aging after ovulation and reduces stimulation to female broodstock. At the same time, sunflower pollen extract is added to the water body where male broodstock is located to promote gonad development and sperm quality in male zebrafish. A specific breeding box is set up to increase the chance of fertilization , compared with the previous patent research, it can significantly improve the fertilization rate of zebrafish eggs, and the operation is convenient.

Description

technical field [0001] The invention relates to the technical field of reproduction and breeding of aquatic animals, in particular to a method for improving the fertilization rate of zebrafish. Background technique [0002] Zebrafish is a subtropical freshwater fish with a small body and elongated and slightly spindle-shaped body. It has 87% high homology with human genes. It has outstanding advantages as a model organism and has high scientific research value. It has been used in retinal restoration research, auditory restoration research, and environmental monitoring. [0003] Zebrafish is a cycle of spawning, in vitro fertilization, and in vitro development. Zebrafish eggs are sticky and will sink to the bottom of the water to avoid being eaten by zebrafish broodstock. In the previous research on the fertilization and reproduction of zebrafish, the applicant developed a breeding box that separates fish eggs from broodstock in order to prevent the problem of low reproduc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01K63/00A01K61/17A01K63/04A23K50/80A23K10/30A61P15/08A61K36/28A61K31/4045
CPCA01K63/003A01K63/04A01K63/042A61K31/4045A61K36/28A23K10/30A23K50/80A01K61/10A01K61/17A61P15/08Y02A40/81Y02A40/818
Inventor 代小新赵波牟毅高阳储张杰刘志强
Owner ZHEJIANG OCEAN UNIV
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