Application of CCFM16 in preparation of bacterial agents, foods or medicines for relieving infantile autism and adsorbing polychlorinated biphenyl

A CCFM16, polychlorinated biphenyl technology, used in food or drug applications, in the field of bacterial agents, can solve problems such as neuronal function damage, achieve social barriers, relieve muscle rigidity and postural gait disorders, and improve stereotyped behaviors Effect

Active Publication Date: 2020-01-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous activity and proliferation of glial cells (astrocytes and microglia) with morphological changes, the release of various pro-inflammatory mediators (cytokines and chemokines) to coordinate immune responses and regulate neuronal function and connectivity, while this response can be neuroprotective (i.e., help repair damaged tissue), chronic dysregulation of CNS cytokines can lead to disruption of neuronal function through their roles in cognition and synaptic regulation, These cytokines include IL-6 and TNF-α, etc.

Method used

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  • Application of CCFM16 in preparation of bacterial agents, foods or medicines for relieving infantile autism and adsorbing polychlorinated biphenyl
  • Application of CCFM16 in preparation of bacterial agents, foods or medicines for relieving infantile autism and adsorbing polychlorinated biphenyl
  • Application of CCFM16 in preparation of bacterial agents, foods or medicines for relieving infantile autism and adsorbing polychlorinated biphenyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Bifidobacterium bifidum CCFM16 has good tolerance to simulated gastrointestinal fluid

[0045] The cryopreserved Bifidobacterium bifidum CCFM16 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and subcultured in mMRS medium for 2~ After 3 times, take 1 mL of the culture solution of Bifidobacterium bifidum CCFM16, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0046] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. Take...

Embodiment 2

[0051] Embodiment 2: Bifidobacterium bifidum CCFM16 has no toxic and side effects to wistar rats

[0052] Bifidobacterium bifidum CCFM16 cells were resuspended in 10% skim milk to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Six male wistar autistic rats with a body weight of about 200 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0053] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 CFU / mL of Bifidobacterium bifidum CCFM16 had no significant impact on rats, no significant changes in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

[0054] Table 3 Changes in body weight and death of mice

[0055]

[0056] Note: -: Rats did not die

Embodiment 3

[0057] Example 3: Bifidobacterium bifidum CCFM16 can significantly improve the autonomous exploration ability of autistic rats

[0058] (1) Breeding of young mice with autism

[0059] Healthy male and female wistar rats were bred and adapted to the environment for 1 week. They were caged together at 5:00 p.m. at a ratio of 2:1, and the vaginal plug was observed at 8:00 the next morning. If no vaginal plug was observed, a vaginal smear was performed. On the first day of pregnancy, the female mice were housed in separate cages after conception, and randomly divided into model group and control group. On the 12.5th day after pregnancy, intraperitoneal injection of sodium valproate (VPA) 600mg / kg (normal saline made into 250mg / mL solution) was given as the model group; Weaning.

[0060]

[0061] (2) Experimental grouping of autistic rats

[0062] Divided into control group, model group and intervention group.

[0063]

[0064]

[0065] (3) Open field test of autistic ...

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Abstract

The invention discloses application of CCFM16 in preparation of bacterial agents, foods or medicines for relieving infantile autism and adsorbing polychlorinated biphenyl. Bifidobacterium bifidum CCFM16 can promote the autonomic behavior and exploratory behavior in an unfamiliar environment caused by infantile autism, relieving impaired social interaction caused by the infantile autism, remarkablyimproving the stereotyped behavior caused by the infantile autism, promoting the cognitive competence of the infantile autism on a new object, improving impaired limb coordination caused by nerve injury, improving normal elongation percentage of a body, relieving myotonia and impaired posture and gait caused by Parkinson's disease and effectively adsorbing polychlorinated biphenyl.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to the application of CCFM16 in the preparation of bacterial agents, food or medicine for relieving autism and adsorbing polychlorinated biphenyls. Background technique [0002] Autism (Autism), also known as autism, has been recognized as a generalized neurodevelopmental disorder that frequently occurs in infants and young children, mainly manifested in social interaction impairment, communication (verbal and non-verbal) function impairment, and repetitive stereotyped behaviors. , often accompanied by a variety of clinical symptoms such as mental retardation, epilepsy or hyperactivity, usually before the age of 3. According to the latest data released by the US Centers for Disease Control and Prevention (CDC), the prevalence of ASD in 2018 was 1:59, a 15% increase from 1:68 in 2016, and the prevalence rate of men was 4 to 5 times that of women. . [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/123A23L11/00A23L19/20A23L29/00A61K35/745A61P25/00C12N1/20C12R1/01A23L11/50
CPCA23C9/1234A23L19/20A23L29/065A61K35/745A61P25/00C12N1/20A23V2002/00A23L11/50A23V2200/322A23V2200/30
Inventor 王刚陈卫孔庆敏赵建新张灏
Owner JIANGNAN UNIV
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