Sample bacteria colony positioning device of unicellular sorter and positioning method

Abstract

The invention provides a sample bacteria colony positioning device of a unicellular sorter and a positioning method. The sample bacteria colony positioning device comprises a sample chip, a motion control module, a sample chip fixing mechanism, an imaging module, a computer and a display module, wherein the sample chip fixing mechanism is connected to the motion control module, and can be driven by the motion control module to move along with the motion control module to perform movement in the horizontal direction and in the vertical direction, so that the situation that imaging of a biological sample on the sample chip is clear is guaranteed; and imaging is transmitted to the computer and the display module. According to the sample bacteria colony positioning device disclosed by the invention, automatic positioning of a sample bacteria colony is performed, so that the operation time of a user is greatly saved; an intelligent positioning process is adopted, so that the operation flowis optimized, and time consumption of manual position aligning is reduced; and the positioning accuracy is high, so that the situations that the observation is inaccurate and the data is wrong generated by manual operation errors are greatly reduced.

Description

technical field [0001] The invention relates to a biological sample positioning device and positioning method, in particular to a sample colony positioning device and positioning method of a single cell sorter. Background technique [0002] The single cell sorter is an instrument used for single cell separation. Before the instrument performs cell sorting operations, the user needs to drop biological samples on the sample chip. Biological samples are composed of cell groups. The single cell sorter can be used to separate Single cells in the cell group are accurately separated into the cell receiving device; [0003] Since the same sample chip is often dripped with different types of samples for sorting, it is necessary to add auxiliary marks on the sample chip to facilitate the user to select the position, distinguish different types of samples, and save operating time; The cells are detected and analyzed on the equipment, and the cells that meet the detection requirements ...

AI Technical Summary

Problems solved by technology

[0004] In the existing technology, in order to achieve rapid positioning of the cells to be detected, the current bio-detection chips mostly use micro-array technology to detect and locate cells and genes, but this method is mainly aimed at the detection method of extraction needle detection, which is not applicable to The rapid positioning operation on the sorting instrument, for example: the patent number is CN201620713125.3, and the title of the invention is: a patent for positioning and detecting biochips, including a chip body, and through holes are evenly opened on the chip body. The opening at the upper end of the through hole is the drop opening, and the opening at the lower end of the through hole is the extraction opening, the drip opening is larger than the extraction opening, the drop opening gradually decreases from the extraction opening, and the inner wall of the through hole is provided with a passivation layer , the surface of the passivation layer is provided with a detection layer, the utility model can accurately locate each object to be detected in the set container area, and the outside of the container area is covered with a passivation layer and a detection layer, so that each detection object is detected Accurately positioned in a single container area for subsequent detection; this patented technology cannot be applied to the sorter for rapid sorting and positioning operations

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