A nucleic acid amplifier heating control device

Abstract

The invention relates to a heating control device for a nucleic acid amplification instrument. The heating control device includes a chip mechanism, a heating module, a heat dissipation module, a transmission mechanism, a heating cover plate mechanism and a control unit. The chip mechanism includes a chip mounting bracket and a chip. The chip is mounted on the chip mounting bracket. The chip includes a reaction bottom plate and capillary tubes. The reaction bottom plate is provided with a groove. The capillary tubes are arranged in the groove. The chip is also provided with at least two temperature sensors. The chip mechanism is also provided with the heating cover plate mechanism which is used for sealing and heating the chip. The heating cover plate mechanism is disposed on the chip mechanism. The transmission mechanism includes a mobile base. The heating module and the heat dissipation module are arranged on the mobile base from top to bottom. The above device can make the PCR response quickly and accurately to achieve temperature rise and fall.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid amplification, and in particular relates to a heating control device for a nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification technology, especially digital polymerase chain reaction technology (Polymerase Chain Reaction referred to as PCR), is a molecular biological technology used to amplify specific DNA molecular fragments. It uses DNA molecules as templates and is artificially synthesized by a pair of The specific oligonucleotide primers can quickly amplify specific DNA molecular fragments through the enzymatic reaction of DNA polymerase, which plays an extremely important role in biology. The basic process of PCR reaction is divided into three steps. The first step is DNA denaturation (94°C), and the hydrogen bond of the double-stranded DNA template is broken under the action of heat to form single-stranded DNA; the second step is annealing (...

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Problems solved by technology

[0005] Although the above patent documents can achieve temperature control to a certain extent, the three stages of the PCR reaction are all performed on the unified heating and cooling module, which will affect the temperature control effect, especially the rapid temperature rise.

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