Enzymatic hydrolysis method of lignocellulose and application of enzymatic hydrolysis method in improving enzymatic hydrolysis efficiency of lignocellulose
A technology of lignocellulose and cellulase, applied in biofuels, fermentation, etc., can solve the problems of increased pretreatment costs, high metal ion concentration, and increased costs, and achieve low inhibitory effect, small residues, and reduced costs Effect
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Embodiment 1
[0048] A method for enzymatic hydrolysis of lignocellulose, comprising the following steps:
[0049] 1) The pretreatment method of lignocellulose: mechanically pulverize lignocellulose, place it at room temperature 20-25°C for 48 hours to dry, and sieve to obtain lignocellulose particles with a particle size of 0.18-0.85mm. In a bottle, put lignocellulose particles into an inorganic salt (water) solution with pH=1 and incubate at 110°C for 1 hour to obtain a solid-liquid mixture, which is separated from the solid-liquid by filtration to obtain a solid, which is then dissolved in water Wash until neutral, dry at 60°C for 24 hours after washing to obtain pretreated lignocellulose, wherein the concentration of the inorganic salt in the inorganic salt solution is 0.05mol / L, and the inorganic salt in the inorganic salt solution is FeCl 3 , the pH of the inorganic salt solution is adjusted by adding HCl solution (the original pH of the inorganic salt solution=2.3).
[0050] The rat...
Embodiment 2
[0057] A method for enzymatic hydrolysis of lignocellulose, comprising the following steps:
[0058] 1) Mechanically pulverize the lignocellulose, place it at a room temperature of 20-25°C for 48 hours to dry, and sieve to obtain lignocellulose particles with a particle size of 0.18-0.85mm. In a screw bottle, put the lignocellulose particles Put it into an inorganic salt solution with pH=1 and keep it warm at 110°C for 1 hour to obtain a solid-liquid mixture. The solid-liquid mixture is separated by filtration to obtain a solid. Wash the solid with water until it is neutral, and dry it at 60°C after washing. 24h, obtain the lignocellulose after pretreatment, wherein, the concentration of inorganic salt in the inorganic salt solution is 0.01mol / L, and the inorganic salt in the inorganic salt solution is FeCl 3 , the pH of the inorganic salt solution is adjusted by adding HCl solution (the original pH of the inorganic salt solution=2.8).
[0059] The ratio of the mass fraction ...
Embodiment 3
[0066] A method for enzymatic hydrolysis of lignocellulose, comprising the following steps:
[0067] 1) Mechanically pulverize the lignocellulose, place it at a room temperature of 20-25°C for 48 hours to dry, and sieve to obtain lignocellulose particles with a particle size of 0.18-0.85mm. In a screw bottle, put the lignocellulose particles Put it into an inorganic salt solution with pH=2 and keep it warm at 110°C for 1 hour to obtain a solid-liquid mixture. The solid-liquid mixture is separated by filtration to obtain a solid. Wash the solid with water until it is neutral, and dry it at 60°C after washing. 24h, obtain the lignocellulose after pretreatment, wherein, the concentration of inorganic salt in the inorganic salt solution is 0.01mol / L, and the inorganic salt in the inorganic salt solution is FeCl 3 , the pH of the inorganic salt solution is adjusted by adding HCl solution (the original pH of the inorganic salt solution=2.8).
[0068] The ratio of the mass fraction ...
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