Composition comprising mannose oligosaccharide and process for making same and use thereof
A technology of mannan oligosaccharide and composition, applied in the field of animal feed, can solve the problems of limitation, impractical application, no consistent composition and the like
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Embodiment 1
[0097] Example 1: Production of MOS composition
[0098] With a weight ratio of 100:3 on a dry basis, a mannose solution (C*TruSweet 016Ko from Cargill) containing 90 wt% + / - 2% mannose on a dry basis was blended with solid citric acid. The resulting solution was evaporated via a thin film evaporator to a dry matter of 85% + / - 1%. The solution was then heated to 165°C by pumping through a micro heat exchanger (Kreuzxtrom-reaktormodul 1694-X-19.0, KIT, IMVT) at a constant flow rate of 15 kg / hr, with a total residence time shorter than 2 seconds. The material was then pumped through a second micro heat exchanger (Kreuzxtrom-reaktormodul 2155-A-4.0, KIT, IWVT) for 11 seconds at a temperature of 195°C, where the main polycondensation reaction occurred.
[0099] The obtained polycondensation product (mannose oligosaccharide composition) was diluted with alkaline water to obtain a solution with a solid content of 50% by weight and a pH of 5+ / -0.2. The solution was then cooled to 50°C....
Embodiment 2
[0100] Example 2: Production of MOS composition
[0101] With a weight ratio of 100:3 on a dry basis, a mannose solution (C*TruSweet 016Ko from Cargill) containing 90 wt% + / - 2% mannose on a dry basis was blended with solid citric acid. The resulting solution was evaporated via a thin film evaporator to a dry matter of 85% + / - 1%. The solution was then heated to 170°C by pumping through a micro heat exchanger (Kreuzxtrom-reaktormodul 1694-X-19.0, KIT, IMVT) at a constant flow rate of 15 kg / hr, with a total residence time shorter than 2 seconds. The material was then pumped through a second micro heat exchanger (Kreuzxtrom-reaktormodul 2155-A-4.0, KIT, IWVT) for 11 seconds at a temperature of 200°C, where the main polycondensation reaction occurred.
[0102] The obtained polycondensation product (mannose oligosaccharide composition) was diluted with alkaline water to obtain a solution with a solid content of 50% by weight and a pH of 5+ / -0.2. The solution was then cooled to 50°C....
Embodiment 3
[0103] Example 3: Comparative example
[0104] The MOS according to the present invention (composition A and composition B prepared according to Examples 1 and 2, respectively) were compared with commercially available yeast-derived MOS compositions (samples C to I).
[0105] The solubility was determined according to Test A as described above.
[0106] The results show that the solubility of all commercial yeast-derived samples is lower than 4wt%, which is much lower than the solubility of the product of the present invention.
[0107] Table 1. Sugar profile and solubility of mannooligosaccharide (MOS) samples determined by HPLC.
[0108]
[0109] Stability to acid and feed enzymes
[0110] Commercially available MOS samples extracted from yeast And according to reality
[0111] The sample of the MOS composition according to the present invention prepared in Example 1 is under conditions of stimulating gastric acid (pH 2 for the first 30 minutes and then pH 7) at 41° C. (the internal ...
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