Flavobacterium columnare transgenic engineering oral vaccine and using method and application thereof

A columnar Flavobacterium and transgenic engineering technology, applied in the fields of molecular biology and aquaculture, can solve problems such as weak stability, potential safety hazards, and reverse mutation, and achieve the effects of reducing fishery economic losses, saving labor, and low production costs

Inactive Publication Date: 2020-02-07
天津市水产研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Flavobacterium columnar inactivated vaccine has complex components, low stability, and the inactivation process will lead to the loss of immunogenicity of some proteins and affect its immune effect
The live attenuated Flavobacterium columnar vaccine has the risk of back mutation, and there are potential safety hazards
The preparation of the recombinant subunit vaccine of Flavobacterium columnar is cumbersome and requires injection immunization, which is difficult in the actual operation process

Method used

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  • Flavobacterium columnare transgenic engineering oral vaccine and using method and application thereof
  • Flavobacterium columnare transgenic engineering oral vaccine and using method and application thereof
  • Flavobacterium columnare transgenic engineering oral vaccine and using method and application thereof

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Embodiment Construction

[0053] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0054] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0055] A kind of Flavobacterium columnar transgenic engineering oral vaccine, the preparation steps are as follows:

[0056] (1) Analyze the mature peptide sequence of the protective antigen gene lip of Flavobacterium columnar, artificially synthesize the mature peptide sequence of the lip gene according to the codon preference of Bacillus subtilis, and add Pst I and SphI restriction sites at both ends of the sequence, And by adding 2 bases at the N-terminal to ensure that the sequence does not shift frame, the stop c...

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Abstract

The invention relates to a flavobacterium columnare transgenic engineering oral vaccine. Preparation steps are as follows: (1) analysis of a mature peptide fragment sequence of a protective antigenicgene, namely lip, of flavobacterium columnare; (2) construction of shuttle plasmids; (3) screening of recombinant strains; (4) culture of the recombinant strains; and (5) preparation of the oral vaccine. The vaccine is an oral vaccine and is inoculated in an oral immunization mode, the oral vaccine is convenient to use compared with injection immunization and immersion immunization vaccines, morelabor force is saved, and the flavobacterium columnare transgenic engineering oral vaccine has the advantage of being not limited by the size of fish bodies.

Description

technical field [0001] The invention belongs to the field of molecular biology and aquatic technology, and relates to aquatic animal immunology, in particular to a Flavobacterium columnar genetically engineered oral vaccine, its use method and application. Background technique [0002] Flavobacterium columnar is a worldwide pathogenic bacterium of aquatic animals, which is a Gram-negative bacillus. Its host range is extremely wide, and it can infect fish of many families including Cyprinidae, Perch, Catfish, Salmonidae, Sunidae, and Mulletidae. Almost all freshwater fish are sensitive to it. In my country, Flavobacterium columnar mainly harms grass carp, mandarin fish, channel catfish, loach, goldfish and other fish, and the columnar disease caused by it is the bacterial disease with the highest incidence rate in freshwater fish, which has caused serious damage to my country's aquaculture industry. harm. [0003] At present, chemical drugs such as antibiotics and disinfecta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P31/04C12N15/75C12N15/66
CPCA61K39/0208A61P31/04C12N15/75C12N15/66A61K2039/552A61K2039/542A61K2039/523C12N2800/22
Inventor 罗璋张振国郝爽白晓慧冯守明
Owner 天津市水产研究所
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