Conformation-specific recombinant A beta 1-42-like oligomer antigen as well as preparation method and application thereof

An oligomer and antigen technology, applied in the fields of biopharmaceuticals and genetic engineering, can solve the problems of inability to improve cognitive ability, inability to effectively neutralize oligomers, and failure to achieve the therapeutic effect of improving cognitive ability.
CN110759987AActive Publication Date: 2020-02-07ACADEMY OF MILITARY MEDICAL SCI
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ACADEMY OF MILITARY MEDICAL SCI
Publication Date
2020-02-07

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a conformation-specific recombinant A beta 1-42-like oligomer antigen as well as a preparation method and an application thereof. The recombinant A beta 1-42-like oligomer antigen is 6 or 12 beta amyloid peptide B cell epitope A beta 1-15 and two helper T cell epitope Th chimeric molecules which are connected in series. The recombinant 6 / 12*(A beta1-15-Th) chimeric antigenimmune serum antibody disclosed by the invention can be specifically combined with an A beta oligomer, the toxicity is neutralized, a high-level Th2 type anti-A beta antibody can be generated by immunizing common and model mice with low dosage and few times, no harmful T cell immune reaction aiming at A beta 1-42 exists, the content of A beta and the oligomer in the brain of an AD mouse is reduced, the related synaptic protein level is up-regulated, and the learning and memory ability is improved. Therefore, the recombinant A beta 1-42-like oligomer antigen targeting the structural characteristics of the A beta oligomer prepared by the invention has great potential and application prospect in AD prevention.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical fields of biopharmaceuticals and genetic engineering, in particular to a conformation-specific recombinant Aβ1-42-like oligomer antigen, a preparation method and application thereof. Background technique

[0002] Alzheimer's disease (Alzheimer's disease, AD), also known as senile dementia, was first discovered by German doctor Alzheimer Alois in 1906. It is a chronic long-term neurodegenerative disease of the elderly. Cognitive and memory impairment due to deletion. According to statistics, the incidence rate of dementia in the elderly aged 60 to 80 is about 4%, while the incidence rate of elderly aged over 80 is as high as 20% to 40%. It is the most common disease among all dementias. According to the latest report of the World Health Organization, there are more than 50 million dementia patients worldwide, and it is expected that this number will triple by 2050. More than 150 million, nearly 10 million new cas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More