A kind of stock solution of plant wall breaking agent and its preparation method and application

A plant-based raw material and plant technology, applied in the field of plant wall breaking, can solve the problems of difficult operation, low wall breaking rate, and high risk of breaking the wall technology, so as to facilitate digestion and absorption of animals, high breaking efficiency, and increase yield Effect

Active Publication Date: 2021-07-27
内蒙古新宏生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem to be solved by the present invention is that the plant cell wall-breaking technology in the prior art may cause damage to the nutrients or pharmaceutical ingredients in the plant cells, the wall-breaking rate is low, and the technical problems of the wall-breaking technology are difficult and risky to operate.

Method used

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  • A kind of stock solution of plant wall breaking agent and its preparation method and application
  • A kind of stock solution of plant wall breaking agent and its preparation method and application
  • A kind of stock solution of plant wall breaking agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Cultivate seed liquid: with 30g soybean peptone, 30g casein peptone, 450g glucose, 100g fructooligosaccharide, 150g isomaltooligosaccharide, 50g xylooligosaccharide, 5g sodium carbonate, 5g sodium chloride, 2g potassium chloride as The main components of the culture medium were dissolved by adding 1000ml of deionized water, then dissolving the auxiliary materials 6g of sodium aluminosilicate, 2g of zinc oxide, 0.01g of silver carbonate, and 20g of chitosan in 500ml of deionized water to make a culture medium. ℃, autoclave for 50 minutes, cool to 37 ℃, inoculate Lactobacillus delbrueckii and Bacillus subtilis strains on the medium for fermentation, and the product is a peptidoglycan complex ion oligomer with a relative molecular weight less than 10KD. Enter sterile compressed air for aerobic fermentation;

[0062] (2) Prepare a fermentation liquid: when the seed liquid OD 450nm = 1.0-2.0, inoculate in the initial fermenter; stop the fermentation when the bacterium g...

Embodiment 2

[0068] (1) Cultivate seed liquid: with 80g soybean peptone, 50g casein peptone, 800g glucose, 400g fructooligosaccharide, 350g isomaltooligosaccharide, 100g xylooligosaccharide, 10g sodium carbonate, 10g sodium chloride, 5g potassium chloride as Add 1000ml of deionized water and stir to dissolve the main components of the culture medium, then dissolve the auxiliary materials 18g of sodium aluminosilicate, 5g of zinc oxide, 0.05g of silver carbonate, and 45g of chitosan in 500ml of deionized water to make a culture medium. ℃, 50 minutes of autoclaving, cooling to 37 ℃, inoculating caseibacterium and bacillus licheniformis in the culture medium for fermentation, the product is peptidoglycan complex ionic oligomer with a relative molecular weight less than 10KD, intermittently fed without Bacteria compressed air for aerobic fermentation;

[0069] (2) Prepare a fermentation liquid: when the seed liquid OD 450nm When = 1.0-2.0, inoculate in the initial fermenter; stop the fermenta...

Embodiment 3

[0075](1) Cultivate seed liquid: with 101g soybean peptone, 90g casein peptone, 1100g glucose, 700g fructooligosaccharide, 850g isomaltooligosaccharide, 200g xylooligosaccharide, 20g sodium carbonate, 20g sodium chloride, 10g potassium chloride as The main components of the culture medium were dissolved by adding 1000ml of deionized water, and then 25g of sodium aluminosilicate, 10g of zinc oxide, 0.5g of silver carbonate, and 99g of chitosan were dissolved in 500ml of deionized water to make a culture medium. ℃, 50 minutes of autoclaving, cooling to 37 ℃, inoculating Lactobacillus plantarum and Bacillus amyloliquefaciens on the culture medium for fermentation, the product is a peptidoglycan complex ion oligomer with a relative molecular weight less than 10KD, intermittently passed Enter sterile compressed air for aerobic fermentation;

[0076] (2) Prepare a fermentation liquid: when the seed liquid OD 450nm When = 1.0-2.0, inoculate in the initial fermenter; stop the ferment...

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Abstract

The invention discloses a stock solution of a plant wall-breaking agent, a preparation method and application thereof, and belongs to the technical field of plant wall-breaking. Through the fermentation process, metal compounds are added to the culture medium, so that the fermentation products of Lactobacillus and Bacillus contain peptidoglycan complex ion oligomers, and Trichoderma reesei is fermented to obtain cellulase and hemicellulase. The stock solution of plant wall-breaking agent includes peptidoglycan complex ion oligomer with relative molecular weight less than 10KD, polypeptide with relative molecular weight less than 3KD, cellulase, hemicellulase, alumino-silica sol inorganic complex-biomacromolecule and water . The preparation process can be continuously fermented, the production process is pipelined, and the production process is pollution-free. When the plant cell wall is broken by the plant wall-breaking agent stock solution of the invention, the wall-breaking rate is higher and the loss of nutrients is small, which can be used for the preparation of premixed feed and the extraction of traditional Chinese medicine, and the product performance is better.

Description

technical field [0001] The invention belongs to the technical field of plant wall breaking, and in particular relates to a plant wall breaking agent stock solution and a preparation method and application thereof. Background technique [0002] Cell wall breaking is an ultrafine pulverization processing technology. If food and traditional Chinese medicine are crushed in a conventional way, a single particle is often composed of several or dozens of particles, and the cell wall breaking rate is extremely low. Cell wall breaking and ultra-grinding processing technology can fully release the active ingredients of grains and Chinese medicinal materials. After the cell wall is broken, the water and oil in the cell move out, so that the surface of the microparticles is in a semi-wet state, and stable particle clusters will be formed between the particles, and each particle cluster contains the same proportion of nutrients or traditional Chinese medicine ingredients. The physical s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/04C12P21/06C12N9/42A23K10/30A23K10/12A61K36/481A61K31/715C08B37/00C12R1/07C12R1/10C12R1/125C12R1/225C12R1/245C12R1/25C12R1/885
CPCA61K31/715A61K36/481A61K2236/19A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55A23K10/12A23K10/30C08B37/0003C12N9/2437C12P19/04C12P21/06C12P39/00
Inventor 高建军张宏
Owner 内蒙古新宏生物科技有限公司
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