Y-STR gene locus and Y-indel gene locus based compound amplification system and primer combinations used by the same

A compound amplification system and primer combination technology, applied in the field of forensic science, can solve the problems of insufficient number of loci and mutation rate of loci, inability to effectively distinguish male individuals, etc.

Active Publication Date: 2020-02-11
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing kit products are insufficient in both the number of loci and the mutation rate of the loci, so that they cannot effectively distinguish male individuals in some case applications

Method used

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  • Y-STR gene locus and Y-indel gene locus based compound amplification system and primer combinations used by the same
  • Y-STR gene locus and Y-indel gene locus based compound amplification system and primer combinations used by the same
  • Y-STR gene locus and Y-indel gene locus based compound amplification system and primer combinations used by the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1, preparation of the multiple amplification system based on 38 Y-STR loci and 3 Y-indel loci

[0113] 1. Screening of Y-STR loci and Y-indel loci

[0114] The inventor of the present invention obtains a large amount of candidate Y-STR locus and Y-indel locus by consulting literature; Then apply the research result of Human Genome Project, in UCSC website (network address is: http: / / genome.ucsc.edu / ) using the reported primer information of each locus to obtain the corresponding sequence; search the sequence in GenBank through BLAST (URL: https: / / blast.ncbi.nlm.nih.gov / Blast.cgi) , and then sort out and systematically study the core and flanking region sequence information, allele length and distribution of the existing Y-STR loci and Y-indel loci, focusing on those whose core sequences are 4-5 base repeats Y-STR loci and Y-indel loci with inserts not smaller than 3bp. Considering technical indicators such as copy number, mutation rate, repeat unit charact...

Embodiment 2

[0134] Embodiment 2, the application of the compound amplification system based on 38 Y-STR loci and 3 Y-indel loci

[0135] The internal standard of Typer500 is the product of the Physical Evidence Appraisal Center of the Ministry of Public Security. Deionized formamide is a product of ABI Company, the product catalog number is 4311320. ABI3130xl Genetic Analyzer is a product of ABI Company. The blood collection card of the person (known as a male) was provided by the Daily DNA Database Construction Institute of the Physical Evidence Identification Center of the Ministry of Public Security.

[0136] The sample to be tested is sample 1 or sample 2:

[0137] Sample 1, 1ng9948 human genomic DNA standard;

[0138] Sample 2: Take a blood collection card from a person (known to be a male), and use a 0.5mm hand-held punch to punch out a disc.

[0139] 1. Take 10 μL of the composite amplification system prepared in step 2 of Example 1, add the sample to be tested for PCR amplific...

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Abstract

The invention discloses a Y-STR gene locus and Y-indel gene locus based compound amplification system and primer combinations used by the same. The provided primer combinations consist of 74 types ofDNA molecules shown as Sequence 1 to Sequence 74 in a sequence table. The STR typing of male individuals can be performed by adopting the primer combinations to build the compound amplification system; and thus, the compound amplification system is high in detected polymorphism, has important identification values on paternity identification, family investigation and other aspects and has significant application values.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a compound amplification system based on Y-STR loci and Y-indel loci and the primer combinations used therefor, in particular to a compound amplification system based on 38 Y-STR loci and 3 Y-indel loci. The multiplex amplification system of the indel locus and the primer combination used. Background technique [0002] Short tandem repeats (short tandem repeats, STRs) belong to the second generation of genetic markers. Due to their small repeat units, high mutation rate, and wide distribution in the genome, they have been widely used in the fields of population genetics and forensic science. In the human genome, there is one STR site about every 15-20kb, and they account for 10% of the total human genome. These STRs usually consist of tandem repeating units of 2-6 bases. Due to the difference in repeating units and repeating times, they show large differenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 尚蕾丁光树莫晓婷余政梁孙辉吴俞衡李万水
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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